Monitoring of yeast cell concentration using a micromachnined impedance sensor

E.E. Krommenhoek, Johannes G.E. Gardeniers, Johan G. Bomer, Albert van den Berg, X. Li, M. Ottens, L.A.M. van der Wielen, G.W.K. van Dedem, M. van Leeuwen, W.M. van Gulik, J.J. Heijnen

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Abstract

This paper describes the design, modeling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in Saccharomyces cerevisiae cell culture show that the characteristic frequency describing the beta-dispersion of S. cerevisiae cells is around 2.8 MHz. The permittivity change of the cell suspension was measured for the concentration range 0-9 g/l and depends linearly on the biomass concentration. In order to compensate the measurements for the electric properties of the background electrolyte, which increases the sensitivity and allows measurements in different media, the use of a three-electrode configuration in combination with a semi-permeable poly(2-hydroxyethyl methacrylate) (pHEMA) membrane was explored. Measurements show that the impedance of hydrated pHEMA varies with the background electrolyte conductivity only, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite-element modelling and was found to be 1 mu m for the electrode configuration under study. (c) 2005 Elsevier B.V. All rights reserved
Original languageEnglish
Pages (from-to)384-389
Number of pages6
JournalSensors and actuators. B: Chemical
Volume115
Issue number1
DOIs
Publication statusPublished - 23 May 2006

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yeast
Yeast
Cells
impedance
Monitoring
sensors
Sensors
biomass
Electrolytes
Biomass
cells
electrolytes
membranes
Membranes
saccharomyces
Electrodes
electrodes
configurations
Cell culture
Assays

Keywords

  • EWI-7601
  • IR-66528
  • METIS-231958

Cite this

Krommenhoek, E.E. ; Gardeniers, Johannes G.E. ; Bomer, Johan G. ; van den Berg, Albert ; Li, X. ; Ottens, M. ; van der Wielen, L.A.M. ; van Dedem, G.W.K. ; van Leeuwen, M. ; van Gulik, W.M. ; Heijnen, J.J. / Monitoring of yeast cell concentration using a micromachnined impedance sensor. In: Sensors and actuators. B: Chemical. 2006 ; Vol. 115, No. 1. pp. 384-389.
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abstract = "This paper describes the design, modeling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in Saccharomyces cerevisiae cell culture show that the characteristic frequency describing the beta-dispersion of S. cerevisiae cells is around 2.8 MHz. The permittivity change of the cell suspension was measured for the concentration range 0-9 g/l and depends linearly on the biomass concentration. In order to compensate the measurements for the electric properties of the background electrolyte, which increases the sensitivity and allows measurements in different media, the use of a three-electrode configuration in combination with a semi-permeable poly(2-hydroxyethyl methacrylate) (pHEMA) membrane was explored. Measurements show that the impedance of hydrated pHEMA varies with the background electrolyte conductivity only, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite-element modelling and was found to be 1 mu m for the electrode configuration under study. (c) 2005 Elsevier B.V. All rights reserved",
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Krommenhoek, EE, Gardeniers, JGE, Bomer, JG, van den Berg, A, Li, X, Ottens, M, van der Wielen, LAM, van Dedem, GWK, van Leeuwen, M, van Gulik, WM & Heijnen, JJ 2006, 'Monitoring of yeast cell concentration using a micromachnined impedance sensor' Sensors and actuators. B: Chemical, vol. 115, no. 1, pp. 384-389. https://doi.org/10.1016/j.snb.2005.09.028

Monitoring of yeast cell concentration using a micromachnined impedance sensor. / Krommenhoek, E.E.; Gardeniers, Johannes G.E.; Bomer, Johan G.; van den Berg, Albert; Li, X.; Ottens, M.; van der Wielen, L.A.M.; van Dedem, G.W.K.; van Leeuwen, M.; van Gulik, W.M.; Heijnen, J.J.

In: Sensors and actuators. B: Chemical, Vol. 115, No. 1, 23.05.2006, p. 384-389.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Monitoring of yeast cell concentration using a micromachnined impedance sensor

AU - Krommenhoek, E.E.

AU - Gardeniers, Johannes G.E.

AU - Bomer, Johan G.

AU - van den Berg, Albert

AU - Li, X.

AU - Ottens, M.

AU - van der Wielen, L.A.M.

AU - van Dedem, G.W.K.

AU - van Leeuwen, M.

AU - van Gulik, W.M.

AU - Heijnen, J.J.

PY - 2006/5/23

Y1 - 2006/5/23

N2 - This paper describes the design, modeling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in Saccharomyces cerevisiae cell culture show that the characteristic frequency describing the beta-dispersion of S. cerevisiae cells is around 2.8 MHz. The permittivity change of the cell suspension was measured for the concentration range 0-9 g/l and depends linearly on the biomass concentration. In order to compensate the measurements for the electric properties of the background electrolyte, which increases the sensitivity and allows measurements in different media, the use of a three-electrode configuration in combination with a semi-permeable poly(2-hydroxyethyl methacrylate) (pHEMA) membrane was explored. Measurements show that the impedance of hydrated pHEMA varies with the background electrolyte conductivity only, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite-element modelling and was found to be 1 mu m for the electrode configuration under study. (c) 2005 Elsevier B.V. All rights reserved

AB - This paper describes the design, modeling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in Saccharomyces cerevisiae cell culture show that the characteristic frequency describing the beta-dispersion of S. cerevisiae cells is around 2.8 MHz. The permittivity change of the cell suspension was measured for the concentration range 0-9 g/l and depends linearly on the biomass concentration. In order to compensate the measurements for the electric properties of the background electrolyte, which increases the sensitivity and allows measurements in different media, the use of a three-electrode configuration in combination with a semi-permeable poly(2-hydroxyethyl methacrylate) (pHEMA) membrane was explored. Measurements show that the impedance of hydrated pHEMA varies with the background electrolyte conductivity only, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite-element modelling and was found to be 1 mu m for the electrode configuration under study. (c) 2005 Elsevier B.V. All rights reserved

KW - EWI-7601

KW - IR-66528

KW - METIS-231958

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DO - 10.1016/j.snb.2005.09.028

M3 - Article

VL - 115

SP - 384

EP - 389

JO - Sensors and actuators. B: Chemical

JF - Sensors and actuators. B: Chemical

SN - 0925-4005

IS - 1

ER -