Monitoring of yeast cell concentration using a micromachnined impedance sensor

E.E. Krommenhoek; Johannes G.E. Gardeniers; Johan G. Bomer; Albert van den Berg; X. Li; M. Ottens; L.A.M. van der Wielen; G.W.K. van Dedem; M. van Leeuwen; W.M. van Gulik; J.J. Heijnen

doi:10.1016/j.snb.2005.09.028

Monitoring of yeast cell concentration using a micromachnined impedance sensor

E.E. Krommenhoek, Johannes G.E. Gardeniers, Johan G. Bomer, Albert van den Berg, X. Li, M. Ottens, L.A.M. van der Wielen, G.W.K. van Dedem, M. van Leeuwen, W.M. van Gulik, J.J. Heijnen

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Abstract

This paper describes the design, modeling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in Saccharomyces cerevisiae cell culture show that the characteristic frequency describing the beta-dispersion of S. cerevisiae cells is around 2.8 MHz. The permittivity change of the cell suspension was measured for the concentration range 0-9 g/l and depends linearly on the biomass concentration. In order to compensate the measurements for the electric properties of the background electrolyte, which increases the sensitivity and allows measurements in different media, the use of a three-electrode configuration in combination with a semi-permeable poly(2-hydroxyethyl methacrylate) (pHEMA) membrane was explored. Measurements show that the impedance of hydrated pHEMA varies with the background electrolyte conductivity only, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite-element modelling and was found to be 1 mu m for the electrode configuration under study. (c) 2005 Elsevier B.V. All rights reserved

Original language	English
Pages (from-to)	384-389
Number of pages	6
Journal	Sensors and Actuators B: Chemical
Volume	115
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2005.09.028
Publication status	Published - 23 May 2006

Keywords

EWI-7601
IR-66528
METIS-231958

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10.1016/j.snb.2005.09.028

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Krommenhoek, E. E., Gardeniers, J. G. E., Bomer, J. G., van den Berg, A., Li, X., Ottens, M., van der Wielen, L. A. M., van Dedem, G. W. K., van Leeuwen, M., van Gulik, W. M., & Heijnen, J. J. (2006). Monitoring of yeast cell concentration using a micromachnined impedance sensor. Sensors and Actuators B: Chemical, 115(1), 384-389. https://doi.org/10.1016/j.snb.2005.09.028

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title = "Monitoring of yeast cell concentration using a micromachnined impedance sensor",

abstract = "This paper describes the design, modeling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in Saccharomyces cerevisiae cell culture show that the characteristic frequency describing the beta-dispersion of S. cerevisiae cells is around 2.8 MHz. The permittivity change of the cell suspension was measured for the concentration range 0-9 g/l and depends linearly on the biomass concentration. In order to compensate the measurements for the electric properties of the background electrolyte, which increases the sensitivity and allows measurements in different media, the use of a three-electrode configuration in combination with a semi-permeable poly(2-hydroxyethyl methacrylate) (pHEMA) membrane was explored. Measurements show that the impedance of hydrated pHEMA varies with the background electrolyte conductivity only, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite-element modelling and was found to be 1 mu m for the electrode configuration under study. (c) 2005 Elsevier B.V. All rights reserved",

keywords = "EWI-7601, IR-66528, METIS-231958",

author = "E.E. Krommenhoek and Gardeniers, {Johannes G.E.} and Bomer, {Johan G.} and {van den Berg}, Albert and X. Li and M. Ottens and {van der Wielen}, L.A.M. and {van Dedem}, G.W.K. and {van Leeuwen}, M. and {van Gulik}, W.M. and J.J. Heijnen",

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doi = "10.1016/j.snb.2005.09.028",

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T1 - Monitoring of yeast cell concentration using a micromachnined impedance sensor

AU - Krommenhoek, E.E.

AU - Gardeniers, Johannes G.E.

AU - Bomer, Johan G.

AU - van den Berg, Albert

AU - Li, X.

AU - Ottens, M.

AU - van der Wielen, L.A.M.

AU - van Dedem, G.W.K.

AU - van Leeuwen, M.

AU - van Gulik, W.M.

AU - Heijnen, J.J.

PY - 2006/5/23

Y1 - 2006/5/23

N2 - This paper describes the design, modeling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in Saccharomyces cerevisiae cell culture show that the characteristic frequency describing the beta-dispersion of S. cerevisiae cells is around 2.8 MHz. The permittivity change of the cell suspension was measured for the concentration range 0-9 g/l and depends linearly on the biomass concentration. In order to compensate the measurements for the electric properties of the background electrolyte, which increases the sensitivity and allows measurements in different media, the use of a three-electrode configuration in combination with a semi-permeable poly(2-hydroxyethyl methacrylate) (pHEMA) membrane was explored. Measurements show that the impedance of hydrated pHEMA varies with the background electrolyte conductivity only, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite-element modelling and was found to be 1 mu m for the electrode configuration under study. (c) 2005 Elsevier B.V. All rights reserved

AB - This paper describes the design, modeling and experimental characterization of a micromachined impedance sensor for on-line monitoring of the viable yeast cell concentration (biomass) in a miniaturized cell assay. Measurements in Saccharomyces cerevisiae cell culture show that the characteristic frequency describing the beta-dispersion of S. cerevisiae cells is around 2.8 MHz. The permittivity change of the cell suspension was measured for the concentration range 0-9 g/l and depends linearly on the biomass concentration. In order to compensate the measurements for the electric properties of the background electrolyte, which increases the sensitivity and allows measurements in different media, the use of a three-electrode configuration in combination with a semi-permeable poly(2-hydroxyethyl methacrylate) (pHEMA) membrane was explored. Measurements show that the impedance of hydrated pHEMA varies with the background electrolyte conductivity only, and not with the concentration of cells, indicating that pHEMA is suitable for this purpose. The optimal pHEMA membrane thickness was determined using finite-element modelling and was found to be 1 mu m for the electrode configuration under study. (c) 2005 Elsevier B.V. All rights reserved

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KW - IR-66528

KW - METIS-231958

U2 - 10.1016/j.snb.2005.09.028

DO - 10.1016/j.snb.2005.09.028

M3 - Article

SN - 0925-4005

VL - 115

SP - 384

EP - 389

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

IS - 1

ER -

Monitoring of yeast cell concentration using a micromachnined impedance sensor

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Keywords

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