Monitoring the respiratory activity of multicellular tumor spheroids in an integrated microfluidic device

Yawar  Abbas; Fleur van Rossem; Johan Bomer; Severine  le Gac

Monitoring the respiratory activity of multicellular tumor spheroids in an integrated microfluidic device

Yawar Abbas, Fleur van Rossem, Johan Bomer, Severine le Gac

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic

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Abstract

We report an integrated sensing device consisting of an ultra-microelectrode array (UMEA) sensor placed in a nanoliter-volume chamber for monitoring the respiratory activity of multicellular tumor spheroids (MCTS). The UMEA sensor is made of platinum, and consists of 16 UMEs of 2 μm diameter spaced by 20 μm. This sensor is integrated in a microfluidic device equipped with trapping structures for capture of one MCTS. The dissolved oxygen concentration is measured using chronoamperometry at a reduction potential of ˗0.6 V by applying voltage pulses for 5 ms. The measurement near MCTS shows a decrease in DO concentration from 7.8 mg/L to 6.7 mg/L within 2 hours, which is caused by the respiration of MCTS.

Original language	English
Title of host publication	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences
Editors	Abraham Lee, Don DeVoe
Publisher	The Chemical and Biological Microsystems Society
Pages	455-456
Number of pages	2
ISBN (Print)	978-0-692-94183-6
Publication status	Published - 22 Oct 2017
Event	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017 - Savannah International Trade & Convention Center, Savannah, United States Duration: 22 Oct 2017 → 26 Oct 2017 Conference number: 21 https://www.microtas2017.org/

Conference

Conference	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017
Abbreviated title	MicroTAS
Country/Territory	United States
City	Savannah
Period	22/10/17 → 26/10/17
Internet address	https://www.microtas2017.org/

Keywords

Oxygen sensing
Multicellular tumor spheroid
Ultra-microelectrode
Chronoamperometry

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Monitoring the respiratory activity of multicellular tumor spheroids in an integrated microfluidic device
Abbas, Y., van Rossem, F., Bomer, J. & le Gac, S., 22 Oct 2017. 2 p.
Research output: Contribution to conference › Poster › Other research output

Cite this

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title = "Monitoring the respiratory activity of multicellular tumor spheroids in an integrated microfluidic device",

abstract = "We report an integrated sensing device consisting of an ultra-microelectrode array (UMEA) sensor placed in a nanoliter-volume chamber for monitoring the respiratory activity of multicellular tumor spheroids (MCTS). The UMEA sensor is made of platinum, and consists of 16 UMEs of 2 μm diameter spaced by 20 μm. This sensor is integrated in a microfluidic device equipped with trapping structures for capture of one MCTS. The dissolved oxygen concentration is measured using chronoamperometry at a reduction potential of ˗0.6 V by applying voltage pulses for 5 ms. The measurement near MCTS shows a decrease in DO concentration from 7.8 mg/L to 6.7 mg/L within 2 hours, which is caused by the respiration of MCTS.",

keywords = "Oxygen sensing, Multicellular tumor spheroid, Ultra-microelectrode, Chronoamperometry",

author = "Yawar Abbas and {van Rossem}, Fleur and Johan Bomer and {le Gac}, Severine",

year = "2017",

month = oct,

day = "22",

language = "English",

isbn = "978-0-692-94183-6",

pages = "455--456",

editor = "Abraham Lee and Don DeVoe",

booktitle = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "The Chemical and Biological Microsystems Society",

note = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017, MicroTAS ; Conference date: 22-10-2017 Through 26-10-2017",

url = "https://www.microtas2017.org/",

}

Abbas, Y, van Rossem, F, Bomer, J & le Gac, S 2017, Monitoring the respiratory activity of multicellular tumor spheroids in an integrated microfluidic device. in A Lee & D DeVoe (eds), 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. The Chemical and Biological Microsystems Society, pp. 455-456, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017, Savannah, Georgia, United States, 22/10/17.

Monitoring the respiratory activity of multicellular tumor spheroids in an integrated microfluidic device. / Abbas, Yawar ; van Rossem, Fleur; Bomer, Johan et al.
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences. ed. / Abraham Lee; Don DeVoe. The Chemical and Biological Microsystems Society, 2017. p. 455-456.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic

TY - GEN

T1 - Monitoring the respiratory activity of multicellular tumor spheroids in an integrated microfluidic device

AU - Abbas, Yawar

AU - van Rossem, Fleur

AU - Bomer, Johan

AU - le Gac, Severine

N1 - Conference code: 21

PY - 2017/10/22

Y1 - 2017/10/22

N2 - We report an integrated sensing device consisting of an ultra-microelectrode array (UMEA) sensor placed in a nanoliter-volume chamber for monitoring the respiratory activity of multicellular tumor spheroids (MCTS). The UMEA sensor is made of platinum, and consists of 16 UMEs of 2 μm diameter spaced by 20 μm. This sensor is integrated in a microfluidic device equipped with trapping structures for capture of one MCTS. The dissolved oxygen concentration is measured using chronoamperometry at a reduction potential of ˗0.6 V by applying voltage pulses for 5 ms. The measurement near MCTS shows a decrease in DO concentration from 7.8 mg/L to 6.7 mg/L within 2 hours, which is caused by the respiration of MCTS.

AB - We report an integrated sensing device consisting of an ultra-microelectrode array (UMEA) sensor placed in a nanoliter-volume chamber for monitoring the respiratory activity of multicellular tumor spheroids (MCTS). The UMEA sensor is made of platinum, and consists of 16 UMEs of 2 μm diameter spaced by 20 μm. This sensor is integrated in a microfluidic device equipped with trapping structures for capture of one MCTS. The dissolved oxygen concentration is measured using chronoamperometry at a reduction potential of ˗0.6 V by applying voltage pulses for 5 ms. The measurement near MCTS shows a decrease in DO concentration from 7.8 mg/L to 6.7 mg/L within 2 hours, which is caused by the respiration of MCTS.

KW - Oxygen sensing

KW - Multicellular tumor spheroid

KW - Ultra-microelectrode

KW - Chronoamperometry

M3 - Conference contribution

SN - 978-0-692-94183-6

SP - 455

EP - 456

BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences

A2 - Lee, Abraham

A2 - DeVoe, Don

PB - The Chemical and Biological Microsystems Society

T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017

Y2 - 22 October 2017 through 26 October 2017

ER -

Monitoring the respiratory activity of multicellular tumor spheroids in an integrated microfluidic device

Abstract

Conference

Keywords

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Research output

Monitoring the respiratory activity of multicellular tumor spheroids in an integrated microfluidic device

Cite this