Monitoring the respiratory activity of multicellular tumor spheroids in an integrated microfluidic device

Yawar Abbas, Fleur van Rossem, Johan Bomer, Severine le Gac

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademic

    Abstract

    We report an integrated sensing device consisting of an ultra-microelectrode array (UMEA) sensor placed in a nanoliter-volume chamber for monitoring the respiratory activity of multicellular tumor spheroids (MCTS). The UMEA sensor is made of platinum, and consists of 16 UMEs of 2 μm diameter spaced by 20 μm. This sensor is integrated in a microfluidic device equipped with trapping structures for capture of one MCTS. The dissolved oxygen concentration is measured using chronoamperometry at a reduction potential of ˗0.6 V by applying voltage pulses for 5 ms. The measurement near MCTS shows a decrease in DO concentration from 7.8 mg/L to 6.7 mg/L within 2 hours, which is caused by the respiration of MCTS.
    Original languageEnglish
    Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences
    EditorsAbraham Lee, Don DeVoe
    PublisherThe Chemical and Biological Microsystems Society
    Pages455-456
    Number of pages2
    ISBN (Print)978-0-692-94183-6
    Publication statusPublished - 22 Oct 2017
    Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017 - Savannah International Trade & Convention Center, Savannah, United States
    Duration: 22 Oct 201726 Oct 2017
    Conference number: 21
    https://www.microtas2017.org/

    Conference

    Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017
    Abbreviated titleMicroTAS
    CountryUnited States
    CitySavannah
    Period22/10/1726/10/17
    Internet address

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    Keywords

    • Oxygen sensing
    • Multicellular tumor spheroid
    • Ultra-microelectrode
    • Chronoamperometry

    Cite this

    Abbas, Y., van Rossem, F., Bomer, J., & le Gac, S. (2017). Monitoring the respiratory activity of multicellular tumor spheroids in an integrated microfluidic device. In A. Lee, & D. DeVoe (Eds.), 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 455-456). The Chemical and Biological Microsystems Society.