We report an integrated sensing device consisting of an ultra-microelectrode array (UMEA) sensor placed in a nanoliter-volume chamber for monitoring the respiratory activity of multicellular tumor spheroids (MCTS). The UMEA sensor is made of platinum, and consists of 16 UMEs of 2 μm diameter spaced by 20 μm. This sensor is integrated in a microfluidic device equipped with trapping structures for capture of one MCTS. The dissolved oxygen concentration is measured using chronoamperometry at a reduction potential of ˗0.6 V by applying voltage pulses for 5 ms. The measurement near MCTS shows a decrease in DO concentration from 7.8 mg/L to 6.7 mg/L within 2 hours, which is caused by the respiration of MCTS.
|Title of host publication||21st International Conference on Miniaturized Systems for Chemistry and Life Sciences|
|Editors||Abraham Lee, Don DeVoe|
|Publisher||The Chemical and Biological Microsystems Society|
|Number of pages||2|
|Publication status||Published - 22 Oct 2017|
|Event||21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017 - Savannah International Trade & Convention Center, Savannah, United States|
Duration: 22 Oct 2017 → 26 Oct 2017
Conference number: 21
|Conference||21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, μTAS 2017|
|Period||22/10/17 → 26/10/17|
- Oxygen sensing
- Multicellular tumor spheroid
Abbas, Y., van Rossem, F., Bomer, J., & le Gac, S. (2017). Monitoring the respiratory activity of multicellular tumor spheroids in an integrated microfluidic device. In A. Lee, & D. DeVoe (Eds.), 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 455-456). The Chemical and Biological Microsystems Society.