MPLA-coated hepatitis B virus surface antigen (HBsAg) nanocapsules induce vigorous T cell responses in cord blood derived human T cells

Anette Pietrzak-Nguyen, Keti Piradashvili, Michael Fichter, Leah Pretsch, Fred Zepp, Frederik R. Wurm, Katharina Landfester, Stephan Gehring*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

Chronic hepatitis B virus (HBV) infection is the most prevalent serious liver infection in the world. A frequent route of infection represents mother-to-child transmission. Efficient control of HBV replication depends on antigen-specific cellular immune response mediated by dendritic cells (DCs). Aim of the present study was to evaluate optimized adjuvant combinations, efficiently maturing monocyte-derived neonatal and adult dendritic cells (moDCs). In addition, the potential of polymeric HBsAg-nanocapsules (HBsAg-NCs) was investigated regarding up-take by moDCs and the subsequent induction of specific T cell responses in a human co-culture model. Simultaneous stimulation of moDCs with MPLA and IFNγ induced up-regulation of CD80 and HLA-DR along with vigorous secretion of IL-12p70. MPLA-coating of HBsAg-NCs promoted NCs-uptake by moDCs. Finally, MPLA-HBsAg-NCs-pulsed moDCs with IFNγ increased T cell proliferation and induced antigen-specific IFNγ release by T cells. The herein presented vaccine approach provides a rational for neonatal and therapeutic immunization strategies against HBV.

Original languageEnglish
Pages (from-to)2383-2394
Number of pages12
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume12
Issue number8
DOIs
Publication statusPublished - 1 Nov 2016
Externally publishedYes

Keywords

  • Co-culture model
  • HBsAg nanocapsules
  • MPLA and IFNγ stimulation
  • Neonatal moDCs
  • T1 immune response

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