TY - JOUR
T1 - Multicore Liquid Perfluorocarbon-Loaded Multimodal Nanoparticles for Stable Ultrasound and 19 F MRI Applied to In Vivo Cell Tracking
AU - Koshkina, Olga
AU - Lajoinie, Guillaume
AU - Baldelli Bombelli, Francesca
AU - Swider, Edyta
AU - Cruz, Luis J.
AU - White, Paul B.
AU - Schweins, Ralf
AU - Dolen, Yusuf
AU - van Dinther, Eric A.W.
AU - van Riessen, N. Koen
AU - Rogers, Sarah E.
AU - Fokkink, Remco
AU - Voets, Ilja K.
AU - van Eck, Ernst R.H.
AU - Heerschap, Arend
AU - Versluis, Michel
AU - de Korte, Chris L.
AU - Figdor, Carl G.
AU - de Vries, I. Jolanda M.
AU - Srinivas, Mangala
PY - 2019/5/9
Y1 - 2019/5/9
N2 -
Ultrasound is the most commonly used clinical imaging modality. However, in applications requiring cell-labeling, the large size and short active lifetime of ultrasound contrast agents limit their longitudinal use. Here, 100 nm radius, clinically applicable, polymeric nanoparticles containing a liquid perfluorocarbon, which enhance ultrasound contrast during repeated ultrasound imaging over the course of at least 48 h, are described. The perfluorocarbon enables monitoring the nanoparticles with quantitative
19
F magnetic resonance imaging, making these particles effective multimodal imaging agents. Unlike typical core–shell perfluorocarbon-based ultrasound contrast agents, these nanoparticles have an atypical fractal internal structure. The nonvaporizing highly hydrophobic perfluorocarbon forms multiple cores within the polymeric matrix and is, surprisingly, hydrated with water, as determined from small-angle neutron scattering and nuclear magnetic resonance spectroscopy. Finally, the nanoparticles are used to image therapeutic dendritic cells with ultrasound in vivo, as well as with
19
F MRI and fluorescence imaging, demonstrating their potential for long-term in vivo multimodal imaging.
AB -
Ultrasound is the most commonly used clinical imaging modality. However, in applications requiring cell-labeling, the large size and short active lifetime of ultrasound contrast agents limit their longitudinal use. Here, 100 nm radius, clinically applicable, polymeric nanoparticles containing a liquid perfluorocarbon, which enhance ultrasound contrast during repeated ultrasound imaging over the course of at least 48 h, are described. The perfluorocarbon enables monitoring the nanoparticles with quantitative
19
F magnetic resonance imaging, making these particles effective multimodal imaging agents. Unlike typical core–shell perfluorocarbon-based ultrasound contrast agents, these nanoparticles have an atypical fractal internal structure. The nonvaporizing highly hydrophobic perfluorocarbon forms multiple cores within the polymeric matrix and is, surprisingly, hydrated with water, as determined from small-angle neutron scattering and nuclear magnetic resonance spectroscopy. Finally, the nanoparticles are used to image therapeutic dendritic cells with ultrasound in vivo, as well as with
19
F MRI and fluorescence imaging, demonstrating their potential for long-term in vivo multimodal imaging.
KW - F MRI
KW - Cell therapy
KW - Cell tracking
KW - Multimodal imaging
KW - Perfluorocarbons
KW - Ultrasound
UR - http://www.scopus.com/inward/record.url?scp=85062980441&partnerID=8YFLogxK
U2 - 10.1002/adfm.201806485
DO - 10.1002/adfm.201806485
M3 - Article
AN - SCOPUS:85062980441
SN - 1616-301X
VL - 29
JO - Advanced functional materials
JF - Advanced functional materials
IS - 19
M1 - 1806485
ER -