Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s as Drug-Releasing Surfaces

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Abstract

Purpose To evaluate the potential of poly(amido amine)-based multilayered thin films in surface mediated drug release. Methods Multilayered thin films were prepared from copolymers of phenylboronic acid-functional poly(amido amine)s and chondroitin sulfate (ChS) in the presence of Alizarin Red S (ARS) as a reporter molecule. Multilayer buildup and ARS incorporation were evaluated with UV–vis spectroscopy. Glucose responsiveness of the multilayers was investigated. Finally, cellular uptake of ARS by COS-7 cells grown on the films was assessed. Results Multilayers based on alcohol containing polymers (ABOL-BA-PAA#ChS + ARS) displayed higher ARS incorporation than multilayers based on amine-containing polymers (DAB-BA-PAA#ChS + ARS). At physiological pH, a swift initial release of up to ~40% of the ARS content was observed during the first 12 h of incubation, followed by a much slower, gradual release of ARS. The multilayers were further evaluated by culturing COS-7 cells on top of multilayer-coated well plates. Cellular uptake of the fluorescent ARS-boronate ester was quantified through flow cytometry, and a maximum uptake of up to 30% was observed. Confocal microscopy confirmed the presence of ARS-boronate ester-containing particles in the nuclei of cells. Conclusions The investigated multilayered thin films are effective in surface-mediated delivery of the model compound ARS. These multilayered surfaces are promising as drug-releasing delivery surface for coating stents, prostheses, and other implants.
Original languageEnglish
Pages (from-to)3732-3745
JournalPharmaceutical research
Volume32
Issue number11
DOIs
Publication statusPublished - 2015

Fingerprint

Boronic Acids
Amines
Thin films
Pharmaceutical Preparations
Multilayers
Chondroitin Sulfates
COS Cells
Polymers
Esters
Alizarin Red S
Stents
Flow cytometry
Confocal microscopy
Cell Nucleus
Confocal Microscopy
Prostheses and Implants
Spectrum Analysis
Flow Cytometry
Copolymers

Keywords

  • METIS-313814
  • IR-99624

Cite this

@article{1b1f1afe2cef476a9447e98ecad01d28,
title = "Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s as Drug-Releasing Surfaces",
abstract = "Purpose To evaluate the potential of poly(amido amine)-based multilayered thin films in surface mediated drug release. Methods Multilayered thin films were prepared from copolymers of phenylboronic acid-functional poly(amido amine)s and chondroitin sulfate (ChS) in the presence of Alizarin Red S (ARS) as a reporter molecule. Multilayer buildup and ARS incorporation were evaluated with UV–vis spectroscopy. Glucose responsiveness of the multilayers was investigated. Finally, cellular uptake of ARS by COS-7 cells grown on the films was assessed. Results Multilayers based on alcohol containing polymers (ABOL-BA-PAA#ChS + ARS) displayed higher ARS incorporation than multilayers based on amine-containing polymers (DAB-BA-PAA#ChS + ARS). At physiological pH, a swift initial release of up to ~40{\%} of the ARS content was observed during the first 12 h of incubation, followed by a much slower, gradual release of ARS. The multilayers were further evaluated by culturing COS-7 cells on top of multilayer-coated well plates. Cellular uptake of the fluorescent ARS-boronate ester was quantified through flow cytometry, and a maximum uptake of up to 30{\%} was observed. Confocal microscopy confirmed the presence of ARS-boronate ester-containing particles in the nuclei of cells. Conclusions The investigated multilayered thin films are effective in surface-mediated delivery of the model compound ARS. These multilayered surfaces are promising as drug-releasing delivery surface for coating stents, prostheses, and other implants.",
keywords = "METIS-313814, IR-99624",
author = "S.D. Hujaya and Engbersen, {Johannes F.J.} and Paulusse, {Jos Marie Johannes}",
note = "Open access",
year = "2015",
doi = "10.1007/s11095-015-1734-y",
language = "English",
volume = "32",
pages = "3732--3745",
journal = "Pharmaceutical research",
issn = "0724-8741",
publisher = "Springer",
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Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s as Drug-Releasing Surfaces. / Hujaya, S.D.; Engbersen, Johannes F.J.; Paulusse, Jos Marie Johannes.

In: Pharmaceutical research, Vol. 32, No. 11, 2015, p. 3732-3745.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s as Drug-Releasing Surfaces

AU - Hujaya, S.D.

AU - Engbersen, Johannes F.J.

AU - Paulusse, Jos Marie Johannes

N1 - Open access

PY - 2015

Y1 - 2015

N2 - Purpose To evaluate the potential of poly(amido amine)-based multilayered thin films in surface mediated drug release. Methods Multilayered thin films were prepared from copolymers of phenylboronic acid-functional poly(amido amine)s and chondroitin sulfate (ChS) in the presence of Alizarin Red S (ARS) as a reporter molecule. Multilayer buildup and ARS incorporation were evaluated with UV–vis spectroscopy. Glucose responsiveness of the multilayers was investigated. Finally, cellular uptake of ARS by COS-7 cells grown on the films was assessed. Results Multilayers based on alcohol containing polymers (ABOL-BA-PAA#ChS + ARS) displayed higher ARS incorporation than multilayers based on amine-containing polymers (DAB-BA-PAA#ChS + ARS). At physiological pH, a swift initial release of up to ~40% of the ARS content was observed during the first 12 h of incubation, followed by a much slower, gradual release of ARS. The multilayers were further evaluated by culturing COS-7 cells on top of multilayer-coated well plates. Cellular uptake of the fluorescent ARS-boronate ester was quantified through flow cytometry, and a maximum uptake of up to 30% was observed. Confocal microscopy confirmed the presence of ARS-boronate ester-containing particles in the nuclei of cells. Conclusions The investigated multilayered thin films are effective in surface-mediated delivery of the model compound ARS. These multilayered surfaces are promising as drug-releasing delivery surface for coating stents, prostheses, and other implants.

AB - Purpose To evaluate the potential of poly(amido amine)-based multilayered thin films in surface mediated drug release. Methods Multilayered thin films were prepared from copolymers of phenylboronic acid-functional poly(amido amine)s and chondroitin sulfate (ChS) in the presence of Alizarin Red S (ARS) as a reporter molecule. Multilayer buildup and ARS incorporation were evaluated with UV–vis spectroscopy. Glucose responsiveness of the multilayers was investigated. Finally, cellular uptake of ARS by COS-7 cells grown on the films was assessed. Results Multilayers based on alcohol containing polymers (ABOL-BA-PAA#ChS + ARS) displayed higher ARS incorporation than multilayers based on amine-containing polymers (DAB-BA-PAA#ChS + ARS). At physiological pH, a swift initial release of up to ~40% of the ARS content was observed during the first 12 h of incubation, followed by a much slower, gradual release of ARS. The multilayers were further evaluated by culturing COS-7 cells on top of multilayer-coated well plates. Cellular uptake of the fluorescent ARS-boronate ester was quantified through flow cytometry, and a maximum uptake of up to 30% was observed. Confocal microscopy confirmed the presence of ARS-boronate ester-containing particles in the nuclei of cells. Conclusions The investigated multilayered thin films are effective in surface-mediated delivery of the model compound ARS. These multilayered surfaces are promising as drug-releasing delivery surface for coating stents, prostheses, and other implants.

KW - METIS-313814

KW - IR-99624

U2 - 10.1007/s11095-015-1734-y

DO - 10.1007/s11095-015-1734-y

M3 - Article

VL - 32

SP - 3732

EP - 3745

JO - Pharmaceutical research

JF - Pharmaceutical research

SN - 0724-8741

IS - 11

ER -