Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s

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Abstract

Purpose To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells. Methods Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films. Results PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10–100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells. Conclusions Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants.
Original languageEnglish
Pages (from-to)3066-3086
JournalPharmaceutical research
Volume32
Issue number9
DOIs
Publication statusPublished - 2015

Fingerprint

Boronic Acids
Amines
Chondroitin Sulfates
COS Cells
Multilayers
Thin films
Biocompatibility
Cell culture
Polymers
Cell Culture Techniques
Alcohols
Glucose
Stents
Reducing Agents
Biocompatible Materials
Disulfides
Experiments
Cells
Pharmaceutical Preparations
benzeneboronic acid

Keywords

  • IR-99625
  • METIS-313816

Cite this

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title = "Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s",
abstract = "Purpose To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells. Methods Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films. Results PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10–100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells. Conclusions Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants.",
keywords = "IR-99625, METIS-313816",
author = "S.D. Hujaya and Engbersen, {Johannes F.J.} and Paulusse, {Jos Marie Johannes}",
note = "Open access",
year = "2015",
doi = "10.1007/s11095-015-1688-0",
language = "English",
volume = "32",
pages = "3066--3086",
journal = "Pharmaceutical research",
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Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s. / Hujaya, S.D.; Engbersen, Johannes F.J.; Paulusse, Jos Marie Johannes.

In: Pharmaceutical research, Vol. 32, No. 9, 2015, p. 3066-3086.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Multilayered Thin Films from Boronic Acid-Functional Poly(amido amine)s

AU - Hujaya, S.D.

AU - Engbersen, Johannes F.J.

AU - Paulusse, Jos Marie Johannes

N1 - Open access

PY - 2015

Y1 - 2015

N2 - Purpose To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells. Methods Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films. Results PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10–100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells. Conclusions Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants.

AB - Purpose To investigate the properties of phenylboronic acid-functional poly(amido amine) polymers (BA-PAA) in forming multilayered thin films with poly(vinyl alcohol) (PVA) and chondroitin sulfate (ChS), and to evaluate their compatibility with COS-7 cells. Methods Copolymers of phenylboronic acid-functional poly(amido amine)s, differing in the content of primary amine (DAB-BA-PAA) or alcohol (ABOL-BA-PAA) side groups, were synthesized and applied in the formation of multilayers with PVA and ChS. Biocompatibility of the resulting films was evaluated through cell culture experiments with COS-7 cells grown on the films. Results PVA-based multilayers were thin, reaching ~100 nm at 10 bilayers, whereas ChS-based multilayers were thick, reaching ~600 nm at the same number of bilayers. All of the multilayers are stable under physiological conditions in vitro and are responsive to reducing agents, owing to the presence of disulfide bonds in the polymers. PVA-based films were demonstrated to be responsive to glucose at physiological pH at the investigated glucose concentrations (10–100 mM). The multilayered films displayed biocompatibility in cell culture experiments, promoting attachment and proliferation of COS-7 cells. Conclusions Responsive thin films based on boronic acid functional poly(amido amine)s are promising biocompatible materials for biomedical applications, such as drug releasing surfaces on stents or implants.

KW - IR-99625

KW - METIS-313816

U2 - 10.1007/s11095-015-1688-0

DO - 10.1007/s11095-015-1688-0

M3 - Article

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SP - 3066

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JO - Pharmaceutical research

JF - Pharmaceutical research

SN - 0724-8741

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