TY - JOUR
T1 - Multimodal Enzyme-Carrying Suprastructures for Rapid and Sensitive Biocatalytic Cascade Reactions
AU - Jo, Seong Min
AU - Kim, Jihye
AU - Lee, Ji Eun
AU - Wurm, Frederik R.
AU - Landfester, Katharina
AU - Wooh, Sanghyuk
N1 - Funding Information:
S.‐M.J., J.K., and J.E.L. contributed equally to this work. This work was financially supported by the National Research Foundation of Korea (NRF) grants (Grant No. NRF‐2019K1A4A7A02113186 and NRF‐2019R1C1C1007560). This work is part of the MaxSynBio consortium, which was jointly funded by the Federal Ministry of Education and Research of Germany and the Max Planck Society. The authors appreciate Prof. Dr. med Volker Mailänder (Universitätsmedizin Mainz, Max Planck Institute for Polymer research) for providing human serum. The authors acknowledge the partner group at the Max Planck Institute for Polymer Research with the Chung‐Ang University. All experiments using human serum were performed in compliance with the relevant laws and institutional guidelines. The institutional ethics committee had approved the study (Landesärztekammer Rheinland‐Pfalz, 837.439.12 (8540‐F)). Written informed consent was obtained for any experimentation with human subjects.
Publisher Copyright:
© 2021 The Authors. Advanced Science published by Wiley-VCH GmbH
PY - 2022/4/5
Y1 - 2022/4/5
N2 - Colloidal assemblies of mesoporous suprastructures provide effective catalysis in an advantageous volume-confined environment. However, typical fabrication methods of colloidal suprastructures are carried out under toxic or harmful conditions for unstable biomolecules, such as, biocatalytic enzymes. For this reason, biocatalytic enzymes have rarely been used with suprastructures, even though biocatalytic cascade reactions in confined environments are more efficient than in open conditions. Here, multimodal enzyme- and photocatalyst-carrying superstructures with efficient cascade reactions for colorimetric glucose detection are demonstrated. The suprastructures consisting of various functional nanoparticles, including enzyme-carrying nanoparticles, are fabricated by surface-templated evaporation driven suprastructure synthesis on polydimethylsiloxane-grafted surfaces at ambient conditions. For the fabrication of suprastructures, no additional chemicals and reactions are required, which allows maintaining the enzyme activities. The multimodal enzymes (glucose oxidase and peroxidase)-carrying suprastructures exhibit rapid and highly sensitive glucose detection via two enzyme cascade reactions in confined geometry. Moreover, the combination of enzymatic and photocatalytic cascade reactions of glucose oxidase to titanium dioxide nanoparticles is successfully realized for the same assay. These results show promising abilities of multiple colloidal mixtures carrying suprastructures for effective enzymatic reactions and open a new door for advanced biological reactions and enzyme-related works.
AB - Colloidal assemblies of mesoporous suprastructures provide effective catalysis in an advantageous volume-confined environment. However, typical fabrication methods of colloidal suprastructures are carried out under toxic or harmful conditions for unstable biomolecules, such as, biocatalytic enzymes. For this reason, biocatalytic enzymes have rarely been used with suprastructures, even though biocatalytic cascade reactions in confined environments are more efficient than in open conditions. Here, multimodal enzyme- and photocatalyst-carrying superstructures with efficient cascade reactions for colorimetric glucose detection are demonstrated. The suprastructures consisting of various functional nanoparticles, including enzyme-carrying nanoparticles, are fabricated by surface-templated evaporation driven suprastructure synthesis on polydimethylsiloxane-grafted surfaces at ambient conditions. For the fabrication of suprastructures, no additional chemicals and reactions are required, which allows maintaining the enzyme activities. The multimodal enzymes (glucose oxidase and peroxidase)-carrying suprastructures exhibit rapid and highly sensitive glucose detection via two enzyme cascade reactions in confined geometry. Moreover, the combination of enzymatic and photocatalytic cascade reactions of glucose oxidase to titanium dioxide nanoparticles is successfully realized for the same assay. These results show promising abilities of multiple colloidal mixtures carrying suprastructures for effective enzymatic reactions and open a new door for advanced biological reactions and enzyme-related works.
KW - biocatalytic cascade reactions
KW - colloidal suprastructures
KW - glucose assays
KW - liquid repellent surfaces
KW - surface-templated suprastructure synthesis
KW - UT-Gold-D
UR - http://www.scopus.com/inward/record.url?scp=85121502395&partnerID=8YFLogxK
U2 - 10.1002/advs.202104884
DO - 10.1002/advs.202104884
M3 - Article
AN - SCOPUS:85121502395
SN - 2198-3844
VL - 9
JO - Advanced science
JF - Advanced science
IS - 10
M1 - 2104884
ER -