Multimodal fluorescence imaging spectroscopy

Martijn H W Stopel, Christian Blum, Vinod Subramaniam

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

1 Citation (Scopus)

Abstract

Multimodal fluorescence imaging is a versatile method that has a wide application range from biological studies to materials science. Typical observables in multimodal fluorescence imaging are intensity, lifetime, excitation, and emission spectra which are recorded at chosen locations at the sample. This chapter describes how to build instrumentation that allows for multimodal fluorescence imaging and explains data analysis procedures for the observables. © 2014 Springer Science+Business Media, LLC.

Original languageEnglish
Title of host publicationFluorescence Spectroscopy and Microscopy
Subtitle of host publicationMethods and Protocols
EditorsYves Engelborghs, Anthonie J.W.G. Visser
PublisherHumana Press
Pages521-536
Number of pages16
ISBN (Electronic)978-1-62703-649-8
ISBN (Print)978-1-62703-648-1
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1076
ISSN (Print)1064-3745

Fingerprint

Multimodal Imaging
Optical Imaging
Fluorescence Spectrometry

Keywords

  • METIS-306438
  • IR-94973

Cite this

Stopel, M. H. W., Blum, C., & Subramaniam, V. (2014). Multimodal fluorescence imaging spectroscopy. In Y. Engelborghs, & A. J. W. G. Visser (Eds.), Fluorescence Spectroscopy and Microscopy: Methods and Protocols (pp. 521-536). (Methods in Molecular Biology ; Vol. 1076). Humana Press. https://doi.org/10.1007/978-1-62703-649-8_23
Stopel, Martijn H W ; Blum, Christian ; Subramaniam, Vinod. / Multimodal fluorescence imaging spectroscopy. Fluorescence Spectroscopy and Microscopy: Methods and Protocols. editor / Yves Engelborghs ; Anthonie J.W.G. Visser. Humana Press, 2014. pp. 521-536 (Methods in Molecular Biology ).
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Stopel, MHW, Blum, C & Subramaniam, V 2014, Multimodal fluorescence imaging spectroscopy. in Y Engelborghs & AJWG Visser (eds), Fluorescence Spectroscopy and Microscopy: Methods and Protocols. Methods in Molecular Biology , vol. 1076, Humana Press, pp. 521-536. https://doi.org/10.1007/978-1-62703-649-8_23

Multimodal fluorescence imaging spectroscopy. / Stopel, Martijn H W; Blum, Christian; Subramaniam, Vinod.

Fluorescence Spectroscopy and Microscopy: Methods and Protocols. ed. / Yves Engelborghs; Anthonie J.W.G. Visser. Humana Press, 2014. p. 521-536 (Methods in Molecular Biology ; Vol. 1076).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

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Stopel MHW, Blum C, Subramaniam V. Multimodal fluorescence imaging spectroscopy. In Engelborghs Y, Visser AJWG, editors, Fluorescence Spectroscopy and Microscopy: Methods and Protocols. Humana Press. 2014. p. 521-536. (Methods in Molecular Biology ). https://doi.org/10.1007/978-1-62703-649-8_23