Multiplexed organ-on-chip device for increased throughput analysis of the tissue barrier function

Mariia Zakharova*, Marinke van der Helm, Marciano Palma do Carmo, Hai Le-The, Martijn Tibbe, Andries van der Meer, Kerensa Broersen, Jan Eijkel, Loes Segerink

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Current organ-on-chip devices suffer from lack of throughput. A multiplexed two-layer device with transparent, through-hole polydimethylsiloxane (PDMS) membrane was developed. This device allows individually addressable cell cultures in a single chip without cross-talk between them. By co-culturing human cerebral microvascular endothelial hCMEC/D3 cells and human astrocytes, the Blood-Brain Barrier was emulated. The visual inspection of cells inside the device was significantly improved with fabricated 2 µm thick membrane.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherThe Chemical and Biological Microsystems Society
Pages432-433
Number of pages2
ISBN (Electronic)9781733419000
Publication statusPublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019 - Congress Center Basel, Basel, Switzerland
Duration: 27 Oct 201931 Oct 2019
Conference number: 23
https://cbmsociety.org/microtas/microtas2019/

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019
Abbreviated titleMicroTAS 2019
CountrySwitzerland
CityBasel
Period27/10/1931/10/19
Internet address

Keywords

  • Blood-Brain Barrier
  • Multiplexed organ-on-chip
  • Thin membrane
  • Throughput

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