Multiplexed organ-on-chip device for increased throughput analysis of the tissue barrier function

Mariia Zakharova; Marinke van der Helm; Marciano Palma do Carmo; Hai Le-The; Martijn Tibbe; Andries van der Meer; Kerensa Broersen; Jan Eijkel; Loes Segerink

Multiplexed organ-on-chip device for increased throughput analysis of the tissue barrier function

Mariia Zakharova^*
, Marinke van der Helm
, Marciano Palma do Carmo
, Hai Le-The
, Martijn Tibbe
, Andries van der Meer
, Kerensa Broersen
, Jan Eijkel
, Loes Segerink

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

Current organ-on-chip devices suffer from lack of throughput. A multiplexed two-layer device with transparent, through-hole polydimethylsiloxane (PDMS) membrane was developed. This device allows individually addressable cell cultures in a single chip without cross-talk between them. By co-culturing human cerebral microvascular endothelial hCMEC/D3 cells and human astrocytes, the Blood-Brain Barrier was emulated. The visual inspection of cells inside the device was significantly improved with fabricated 2 µm thick membrane.

Original language	English
Title of host publication	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Publisher	The Chemical and Biological Microsystems Society
Pages	432-433
Number of pages	2
ISBN (Electronic)	9781733419000
Publication status	Published - 2019
Event	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019 - Congress Center Basel, Basel, Switzerland Duration: 27 Oct 2019 → 31 Oct 2019 Conference number: 23 https://cbmsociety.org/microtas/microtas2019/

Conference

Conference	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019
Abbreviated title	MicroTAS 2019
Country/Territory	Switzerland
City	Basel
Period	27/10/19 → 31/10/19
Internet address	https://cbmsociety.org/microtas/microtas2019/

Keywords

Blood-Brain Barrier
Multiplexed organ-on-chip
Thin membrane
Throughput

Cite this

Zakharova, M., van der Helm, M., do Carmo, M. P., Le-The, H., Tibbe, M., van der Meer, A., Broersen, K., Eijkel, J., & Segerink, L. (2019). Multiplexed organ-on-chip device for increased throughput analysis of the tissue barrier function. In 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 (pp. 432-433). The Chemical and Biological Microsystems Society.

@inproceedings{a7da84a46d10407ab37177cdf476a5fe,

title = "Multiplexed organ-on-chip device for increased throughput analysis of the tissue barrier function",

abstract = "Current organ-on-chip devices suffer from lack of throughput. A multiplexed two-layer device with transparent, through-hole polydimethylsiloxane (PDMS) membrane was developed. This device allows individually addressable cell cultures in a single chip without cross-talk between them. By co-culturing human cerebral microvascular endothelial hCMEC/D3 cells and human astrocytes, the Blood-Brain Barrier was emulated. The visual inspection of cells inside the device was significantly improved with fabricated 2 µm thick membrane.",

keywords = "Blood-Brain Barrier, Multiplexed organ-on-chip, Thin membrane, Throughput",

author = "Mariia Zakharova and \{van der Helm\}, Marinke and \{do Carmo\}, \{Marciano Palma\} and Hai Le-The and Martijn Tibbe and \{van der Meer\}, Andries and Kerensa Broersen and Jan Eijkel and Loes Segerink",

year = "2019",

language = "English",

pages = "432--433",

booktitle = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019",

publisher = "The Chemical and Biological Microsystems Society",

note = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019, MicroTAS 2019 ; Conference date: 27-10-2019 Through 31-10-2019",

url = "https://cbmsociety.org/microtas/microtas2019/",

}

Zakharova, M, van der Helm, M, do Carmo, MP, Le-The, H, Tibbe, M, van der Meer, A , Broersen, K , Eijkel, J & Segerink, L 2019, Multiplexed organ-on-chip device for increased throughput analysis of the tissue barrier function. in 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. The Chemical and Biological Microsystems Society, pp. 432-433, 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019, Basel, Switzerland, 27/10/19.

Multiplexed organ-on-chip device for increased throughput analysis of the tissue barrier function. / Zakharova, Mariia; van der Helm, Marinke; do Carmo, Marciano Palma et al.
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. The Chemical and Biological Microsystems Society, 2019. p. 432-433.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Multiplexed organ-on-chip device for increased throughput analysis of the tissue barrier function

AU - Zakharova, Mariia

AU - van der Helm, Marinke

AU - do Carmo, Marciano Palma

AU - Le-The, Hai

AU - Tibbe, Martijn

AU - van der Meer, Andries

AU - Broersen, Kerensa

AU - Eijkel, Jan

AU - Segerink, Loes

N1 - Conference code: 23

PY - 2019

Y1 - 2019

N2 - Current organ-on-chip devices suffer from lack of throughput. A multiplexed two-layer device with transparent, through-hole polydimethylsiloxane (PDMS) membrane was developed. This device allows individually addressable cell cultures in a single chip without cross-talk between them. By co-culturing human cerebral microvascular endothelial hCMEC/D3 cells and human astrocytes, the Blood-Brain Barrier was emulated. The visual inspection of cells inside the device was significantly improved with fabricated 2 µm thick membrane.

AB - Current organ-on-chip devices suffer from lack of throughput. A multiplexed two-layer device with transparent, through-hole polydimethylsiloxane (PDMS) membrane was developed. This device allows individually addressable cell cultures in a single chip without cross-talk between them. By co-culturing human cerebral microvascular endothelial hCMEC/D3 cells and human astrocytes, the Blood-Brain Barrier was emulated. The visual inspection of cells inside the device was significantly improved with fabricated 2 µm thick membrane.

KW - Blood-Brain Barrier

KW - Multiplexed organ-on-chip

KW - Thin membrane

KW - Throughput

UR - https://www.scopus.com/pages/publications/85094945055

M3 - Conference contribution

AN - SCOPUS:85094945055

SP - 432

EP - 433

BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

PB - The Chemical and Biological Microsystems Society

T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2019

Y2 - 27 October 2019 through 31 October 2019

ER -

Multiplexed organ-on-chip device for increased throughput analysis of the tissue barrier function

Abstract

Conference

Keywords

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