Multipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds

K.E.M. Benders, W. Boot, S.M. Cokelaere, P.R. van Weeren, D. Gawlitta, H.J. Bergman, Daniël B.F. Saris, W.J.A. Dhert, J. Malda

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26 Citations (Scopus)

Abstract

Objective. Although extracellular matrix (ECM)–derived scaffolds have been extensively studied and applied in a number of clinical applications, the use of ECM as a biomaterial for (osteo)chondral regeneration is less extensively explored. This study aimed at evaluating the chondrogenic potential of cells seeded on cartilage-derived matrix (CDM) scaffolds in vitro. Design. Scaffolds were generated from decellularized equine articular cartilage and seeded with either chondrocytes or multipotent stromal cells (MSCs). After 2, 4, and 6 weeks of in vitro culture, CDM constructs were analyzed both histologically (hematoxylin and eosin, Safranin-O, collagen types I and II) and biochemically (glycosaminoglycan [GAG] and DNA content). Results. After 4 weeks, both cell types demonstrated chondrogenic differentiation; however, the MSCs significantly outperformed chondrocytes in producing new GAG-containing cartilaginous matrix. Conclusion. These promising in vitro results underscore the potency of CDM scaffolds in (osteo)chondral defect repair
Original languageEnglish
Pages (from-to)221-230
JournalCartilage
Volume5
Issue number4
DOIs
Publication statusPublished - 2014

Keywords

  • IR-96030
  • METIS-310652

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