Multistep Energy Transfer in Single Molecular Photonic Wires

Mike Heilemann, Philip Tinnefeld, G. ir. Sanchez Mosteiro, Gabriel Sanchez Mosteiro, M.F. Garcia Parajo, N.F. van Hulst, Markus Sauer

Research output: Contribution to journalArticleAcademicpeer-review

169 Citations (Scopus)

Abstract

We demonstrate the synthesis and spectroscopic characterization of an unidirectional photonic wire based on four highly efficient fluorescence energy-transfer steps (FRET) between five spectrally different chromophores covalently attached to double-stranded DNA. The DNA-based modular conception enables the introduction of various chromophores at well-defined positions and arbitrary interchromophore distances. While ensemble fluorescence measurements show overall FRET efficiencies between 15 and 30%, single-molecule spectroscopy performed on four spectrally separated detectors easily uncovers subpopulations that exhibit overall FRET efficiencies of up to ~90% across a distance of 13.6 nm and a spectral range of ~200 nm. Fluorescence trajectories of individual photonic wires show five different fluorescence intensity patterns which can be ascribed to successive photobleaching events.
Original languageUndefined
Pages (from-to)6514-6515
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number21
DOIs
Publication statusPublished - 2004

Keywords

  • METIS-218155
  • IR-47574

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