Multivalent Binding of Small Guest Molecules and Proteins to Molecular Printboards inside Microchannels

M.J.W. Ludden, X.Y. Ling, T. Gang, W.P. Bula, Johannes G.E. Gardeniers, David Reinhoudt, Jurriaan Huskens

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β-Cyclodextrin (β-CD) monolayers have been immobilized in microchannels. The host-guest interactions on the -CD monolayers inside the channels were comparable to the interactions on β-CD monolayers on planar surfaces, and a divalent fluorescent guest attached with a comparable binding strength. Proteins were attached to these monolayers inside microchannels in a selective manner by employing a strategy that uses streptavidin and orthogonal linker molecules. The design of the chip, which involved a large channel that splits into four smaller channels, allowed the channels to be addressed separately and led to the selective immobilization of antibodies. Experiments with labeled antibodies showed the selective immobilization of these antibodies in the separate channels.
Original languageUndefined
Pages (from-to)136-142
Number of pages7
JournalChemistry : a European journal
Issue number1
Publication statusPublished - 2008


  • IR-72205
  • METIS-245014
  • lab-on-a-chip devices
  • Host-guest systems
  • Monolayers
  • Cyclodextrins

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