We show an approach based on a combination of site-directed mutagenesis, NIL and multivalent host−guest interactions for the realization of engineered ordered functional arrays of purified components of the photosynthetic system, the membrane-bound LH2 complex. In addition to micrometer-scale patterned structures, we demonstrated the use of nanometer-scale hard NIL stamps to generate functional protein arrays approaching molecular dimensions.
Escalante Marun, M., Zhao, Y., Ludden, M. J. W., Vermeij, R. J., Olsen, J. D., Berenschot, J. W., ... Otto, C. (2008). Nanometer arrays of functional light harvesting antenna complexes by nanoimprint lithography and host--guest interactions. Journal of the American Chemical Society, 130(28), 8892-8893. https://doi.org/10.1021/ja802843m