Nanoparticle Aggregate-Based Fluorescence Enhancement for Highly Sensitive and Reproducible Detection of DNA

C. Annink; Ron Gill

doi:10.1002/ppsc.201400061

Nanoparticle Aggregate-Based Fluorescence Enhancement for Highly Sensitive and Reproducible Detection of DNA

C. Annink, Ron Gill

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Sensitive detection of DNA at the sub picomolar range is demonstrated using a magnetic bead sandwich hybridization assay coupled with surface-enhanced fluorescence (SEF)-based amplification. Unlike enzymatic amplification, the SEF amplification step does not add any additional background to the measurement and does not cause a significant increase in the coefficient of variance (CV).

Original language	Undefined
Pages (from-to)	943-947
Number of pages	5
Journal	Particle & particle systems characterization
Volume	31
Issue number	9
DOIs	https://doi.org/10.1002/ppsc.201400061
Publication status	Published - 2014

Keywords

METIS-306143
IR-94933

Access to Document

10.1002/ppsc.201400061

Cite this

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title = "Nanoparticle Aggregate-Based Fluorescence Enhancement for Highly Sensitive and Reproducible Detection of DNA",

abstract = "Sensitive detection of DNA at the sub picomolar range is demonstrated using a magnetic bead sandwich hybridization assay coupled with surface-enhanced fluorescence (SEF)-based amplification. Unlike enzymatic amplification, the SEF amplification step does not add any additional background to the measurement and does not cause a significant increase in the coefficient of variance (CV).",

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author = "C. Annink and Ron Gill",

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AU - Gill, Ron

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AB - Sensitive detection of DNA at the sub picomolar range is demonstrated using a magnetic bead sandwich hybridization assay coupled with surface-enhanced fluorescence (SEF)-based amplification. Unlike enzymatic amplification, the SEF amplification step does not add any additional background to the measurement and does not cause a significant increase in the coefficient of variance (CV).

KW - METIS-306143

KW - IR-94933

U2 - 10.1002/ppsc.201400061

DO - 10.1002/ppsc.201400061

M3 - Article

SN - 0934-0866

VL - 31

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JO - Particle & particle systems characterization

JF - Particle & particle systems characterization

IS - 9

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