Nanoparticles for intravascular applications: physicochemical characterization and cytotoxicity testing

J. Matuszak; J. Baumgartner; J. Zaloga; M. Juenet; A.E. Da Silva; D. Franke; G. Almer; I. Texier; D. Faivre; Josbert Maarten Metselaar; F.P. Navarro; C. Chauvierre; R. Prassl; L. Dézsi; R. Urbanics; C. Alexiou; H. Mangge; J. Szebeni; D. Letourneur; I. Cicha

doi:10.2217/nnm.15.216

Nanoparticles for intravascular applications: physicochemical characterization and cytotoxicity testing

J. Matuszak, J. Baumgartner, J. Zaloga, M. Juenet, A.E. Da Silva, D. Franke, G. Almer, I. Texier, D. Faivre, Josbert Maarten Metselaar, F.P. Navarro, C. Chauvierre, R. Prassl, L. Dézsi, R. Urbanics, C. Alexiou, H. Mangge, J. Szebeni, D. Letourneur, I. Cicha

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Abstract

Aim: We report the physicochemical analysis of nanosystems intended for cardiovascular applications and their toxicological characterization in static and dynamic cell culture conditions. Methods: Size, polydispersity and ζ-potential were determined in 10 nanoparticle systems including liposomes, lipid nanoparticles, polymeric and iron oxide nanoparticles. Nanoparticle effects on primary human endothelial cell viability were monitored using real-time cell analysis and live-cell microscopy in static conditions, and in a flow model of arterial bifurcations. Results & conclusions: The majority of tested nanosystems were well tolerated by endothelial cells up to the concentration of 100 μg/ml in static, and up to 400 μg/ml in dynamic conditions. Pilot experiments in a pig model showed that intravenous administration of liposomal nanoparticles did not evoke the hypersensitivity reaction. These findings are of importance for future clinical use of nanosystems intended for intravascular applications.

Original language	English
Pages (from-to)	597-616
Journal	Nanomedicine
Volume	11
Issue number	6
DOIs	https://doi.org/10.2217/nnm.15.216
Publication status	Published - 2016

Keywords

METIS-321012
IR-103925

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Matuszak, J., Baumgartner, J., Zaloga, J., Juenet, M., Da Silva, A. E., Franke, D., Almer, G., Texier, I., Faivre, D., Metselaar, J. M., Navarro, F. P., Chauvierre, C., Prassl, R., Dézsi, L., Urbanics, R., Alexiou, C., Mangge, H., Szebeni, J., Letourneur, D., & Cicha, I. (2016). Nanoparticles for intravascular applications: physicochemical characterization and cytotoxicity testing. Nanomedicine, 11(6), 597-616. https://doi.org/10.2217/nnm.15.216

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title = "Nanoparticles for intravascular applications: physicochemical characterization and cytotoxicity testing",

abstract = "Aim: We report the physicochemical analysis of nanosystems intended for cardiovascular applications and their toxicological characterization in static and dynamic cell culture conditions. Methods: Size, polydispersity and ζ-potential were determined in 10 nanoparticle systems including liposomes, lipid nanoparticles, polymeric and iron oxide nanoparticles. Nanoparticle effects on primary human endothelial cell viability were monitored using real-time cell analysis and live-cell microscopy in static conditions, and in a flow model of arterial bifurcations. Results & conclusions: The majority of tested nanosystems were well tolerated by endothelial cells up to the concentration of 100 μg/ml in static, and up to 400 μg/ml in dynamic conditions. Pilot experiments in a pig model showed that intravenous administration of liposomal nanoparticles did not evoke the hypersensitivity reaction. These findings are of importance for future clinical use of nanosystems intended for intravascular applications.",

keywords = "METIS-321012, IR-103925",

author = "J. Matuszak and J. Baumgartner and J. Zaloga and M. Juenet and {Da Silva}, A.E. and D. Franke and G. Almer and I. Texier and D. Faivre and Metselaar, {Josbert Maarten} and F.P. Navarro and C. Chauvierre and R. Prassl and L. D{\'e}zsi and R. Urbanics and C. Alexiou and H. Mangge and J. Szebeni and D. Letourneur and I. Cicha",

year = "2016",

doi = "10.2217/nnm.15.216",

language = "English",

volume = "11",

pages = "597--616",

journal = "Nanomedicine",

issn = "1743-5889",

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number = "6",

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Matuszak, J, Baumgartner, J, Zaloga, J, Juenet, M, Da Silva, AE, Franke, D, Almer, G, Texier, I, Faivre, D, Metselaar, JM, Navarro, FP, Chauvierre, C, Prassl, R, Dézsi, L, Urbanics, R, Alexiou, C, Mangge, H, Szebeni, J, Letourneur, D & Cicha, I 2016, 'Nanoparticles for intravascular applications: physicochemical characterization and cytotoxicity testing', Nanomedicine, vol. 11, no. 6, pp. 597-616. https://doi.org/10.2217/nnm.15.216

TY - JOUR

T1 - Nanoparticles for intravascular applications: physicochemical characterization and cytotoxicity testing

AU - Matuszak, J.

AU - Baumgartner, J.

AU - Zaloga, J.

AU - Juenet, M.

AU - Da Silva, A.E.

AU - Franke, D.

AU - Almer, G.

AU - Texier, I.

AU - Faivre, D.

AU - Metselaar, Josbert Maarten

AU - Navarro, F.P.

AU - Chauvierre, C.

AU - Prassl, R.

AU - Dézsi, L.

AU - Urbanics, R.

AU - Alexiou, C.

AU - Mangge, H.

AU - Szebeni, J.

AU - Letourneur, D.

AU - Cicha, I.

PY - 2016

Y1 - 2016

N2 - Aim: We report the physicochemical analysis of nanosystems intended for cardiovascular applications and their toxicological characterization in static and dynamic cell culture conditions. Methods: Size, polydispersity and ζ-potential were determined in 10 nanoparticle systems including liposomes, lipid nanoparticles, polymeric and iron oxide nanoparticles. Nanoparticle effects on primary human endothelial cell viability were monitored using real-time cell analysis and live-cell microscopy in static conditions, and in a flow model of arterial bifurcations. Results & conclusions: The majority of tested nanosystems were well tolerated by endothelial cells up to the concentration of 100 μg/ml in static, and up to 400 μg/ml in dynamic conditions. Pilot experiments in a pig model showed that intravenous administration of liposomal nanoparticles did not evoke the hypersensitivity reaction. These findings are of importance for future clinical use of nanosystems intended for intravascular applications.

AB - Aim: We report the physicochemical analysis of nanosystems intended for cardiovascular applications and their toxicological characterization in static and dynamic cell culture conditions. Methods: Size, polydispersity and ζ-potential were determined in 10 nanoparticle systems including liposomes, lipid nanoparticles, polymeric and iron oxide nanoparticles. Nanoparticle effects on primary human endothelial cell viability were monitored using real-time cell analysis and live-cell microscopy in static conditions, and in a flow model of arterial bifurcations. Results & conclusions: The majority of tested nanosystems were well tolerated by endothelial cells up to the concentration of 100 μg/ml in static, and up to 400 μg/ml in dynamic conditions. Pilot experiments in a pig model showed that intravenous administration of liposomal nanoparticles did not evoke the hypersensitivity reaction. These findings are of importance for future clinical use of nanosystems intended for intravascular applications.

KW - METIS-321012

KW - IR-103925

U2 - 10.2217/nnm.15.216

DO - 10.2217/nnm.15.216

M3 - Article

VL - 11

SP - 597

EP - 616

JO - Nanomedicine

JF - Nanomedicine

SN - 1743-5889

IS - 6

ER -

Nanoparticles for intravascular applications: physicochemical characterization and cytotoxicity testing

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