TY - JOUR
T1 - Nanoparticles for “two color” 19F magnetic resonance imaging
T2 - Towards combined imaging of biodistribution and degradation
AU - Koshkina, Olga
AU - White, Paul B.
AU - Staal, Alexander H.J.
AU - Schweins, Ralf
AU - Swider, Edyta
AU - Tirotta, Ilaria
AU - Tinnemans, Paul
AU - Fokkink, Remco
AU - Veltien, Andor
AU - van Riessen, N. Koen
AU - van Eck, Ernst R.H.
AU - Heerschap, Arend
AU - Metrangolo, Pierangelo
AU - Baldelli Bombelli, Francesca
AU - Srinivas, Mangala
N1 - Funding Information:
This work was supported by ERC-2014-StG-336454-CoNQUeST . FBB and PM are thankful for funding to Regione Lombardia (Fondo per lo Sviluppo e la Coesione – FAS 2007–2013) and the project 2015-0023-TRANS-ALS is also acknowledged. SANS was supported by the Institut Laue-Langevin (ILL) (doi: https://doi.org//10.5291/ILL-DATA.9-10-1478). Experiments at the ISIS Pulsed Neutron and Muon Source were supported by a beamtime allocation from the Science and Technology Facilities Council. OK acknowledges Erasmus + staff travel grant for support of travel to ISIS neutron source. TTW-NWO open technology grant STW-14716.
Funding Information:
This work was supported by ERC-2014-StG-336454-CoNQUeST. FBB and PM are thankful for funding to Regione Lombardia (Fondo per lo Sviluppo e la Coesione – FAS 2007–2013) and the project 2015-0023-TRANS-ALS is also acknowledged. SANS was supported by the Institut Laue-Langevin (ILL) (doi: https://doi.org//10.5291/ILL-DATA.9-10-1478). Experiments at the ISIS Pulsed Neutron and Muon Source were supported by a beamtime allocation from the Science and Technology Facilities Council. OK acknowledges Erasmus + staff travel grant for support of travel to ISIS neutron source. TTW-NWO open technology grant STW-14716.
Publisher Copyright:
© 2019
PY - 2020/4/1
Y1 - 2020/4/1
N2 - The use of polymeric nanoparticles (NPs) as therapeutics has been steadily increasing over past decades. In vivo imaging of NPs is necessary to advance the therapeutic performance. 19F Magnetic Resonance Imaging (19F MRI) offers multiple advantages for in vivo imaging. However, design of a probe for both biodistribution and degradation has not been realized yet. We developed polymeric NPs loaded with two fluorocarbons as promising imaging tools to monitor NP biodistribution and degradation by 19F MRI. These 200 nm NPs consist of poly(lactic-co-glycolic acid) (PLGA) loaded with perfluoro-15-crown-5 ether (PFCE) and PERFECTA. PERFECTA/PFCE-PLGA NPs have a fractal sphere structure, in which both fluorocarbons are distributed in the polymeric matrix of the fractal building blocks, which differs from PFCE-PLGA NPs and is unique for fluorocarbon-loaded colloids. This structure leads to changes of magnetic resonance properties of both fluorocarbons after hydrolysis of NPs. PERFECTA/PFCE-PLGA NPs are colloidally stable in serum and biocompatible. Both fluorocarbons show a single resonance in 19F MRI that can be imaged separately using different excitation pulses. In the future, these findings may be used for biodistribution and degradation studies of NPs by 19F MRI in vivo using “two color” labeling leading to improvement of drug delivery agents.
AB - The use of polymeric nanoparticles (NPs) as therapeutics has been steadily increasing over past decades. In vivo imaging of NPs is necessary to advance the therapeutic performance. 19F Magnetic Resonance Imaging (19F MRI) offers multiple advantages for in vivo imaging. However, design of a probe for both biodistribution and degradation has not been realized yet. We developed polymeric NPs loaded with two fluorocarbons as promising imaging tools to monitor NP biodistribution and degradation by 19F MRI. These 200 nm NPs consist of poly(lactic-co-glycolic acid) (PLGA) loaded with perfluoro-15-crown-5 ether (PFCE) and PERFECTA. PERFECTA/PFCE-PLGA NPs have a fractal sphere structure, in which both fluorocarbons are distributed in the polymeric matrix of the fractal building blocks, which differs from PFCE-PLGA NPs and is unique for fluorocarbon-loaded colloids. This structure leads to changes of magnetic resonance properties of both fluorocarbons after hydrolysis of NPs. PERFECTA/PFCE-PLGA NPs are colloidally stable in serum and biocompatible. Both fluorocarbons show a single resonance in 19F MRI that can be imaged separately using different excitation pulses. In the future, these findings may be used for biodistribution and degradation studies of NPs by 19F MRI in vivo using “two color” labeling leading to improvement of drug delivery agents.
KW - F MRI
KW - Degradation
KW - Fluorocarbons
KW - Fractal nanoparticles
KW - PLGA
KW - SANS
UR - http://www.scopus.com/inward/record.url?scp=85078551036&partnerID=8YFLogxK
U2 - 10.1016/j.jcis.2019.12.083
DO - 10.1016/j.jcis.2019.12.083
M3 - Article
C2 - 31978790
VL - 565
SP - 278
EP - 287
JO - Journal of colloid and interface science
JF - Journal of colloid and interface science
SN - 0021-9797
ER -