Nanoreactors via encapsulation of catalytic gold nanoparticles within cowpea chlorotic mottle virus protein cages

Aijie Liu, Mark V. de Ruiter, Stan J. Maassen, Jeroen J.L.M. Cornelissen

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

Viral protein cage-based nanoreactors can be generated by encapsulation of catalytic metal nanoparticles within the capsid structure. In this method, coat proteins of the cowpea chlorotic mottle virus (CCMV) are used to sequester gold nanoparticles (Au NPs) in buffered solutions at neutral pH to form CCMV-Au hybrid nanoparticles. This chapter describes detailed methods for the encapsulation of Au NPs into CCMV protein cages. Protocols for the reduction of nitroarenes by using CCMV-Au NPs as catalyst are described as an example for the catalytic activity of Au NPs in the protein cages.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages1-9
Number of pages9
Volume1798
DOIs
Publication statusPublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1798
ISSN (Print)1064-3745

Fingerprint

Bromovirus
Gold
Nanoparticles
Proteins
Metal Nanoparticles
Capsid
Capsid Proteins
Viral Proteins

Keywords

  • Catalysis
  • CCMV
  • Encapsulation
  • Gold nanoparticle
  • Nanoreactor
  • Protein cage

Cite this

Liu, A., de Ruiter, M. V., Maassen, S. J., & Cornelissen, J. J. L. M. (2018). Nanoreactors via encapsulation of catalytic gold nanoparticles within cowpea chlorotic mottle virus protein cages. In Methods in Molecular Biology (Vol. 1798, pp. 1-9). (Methods in Molecular Biology; Vol. 1798). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7893-9_1
Liu, Aijie ; de Ruiter, Mark V. ; Maassen, Stan J. ; Cornelissen, Jeroen J.L.M. / Nanoreactors via encapsulation of catalytic gold nanoparticles within cowpea chlorotic mottle virus protein cages. Methods in Molecular Biology. Vol. 1798 Humana Press Inc., 2018. pp. 1-9 (Methods in Molecular Biology).
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Liu, A, de Ruiter, MV, Maassen, SJ & Cornelissen, JJLM 2018, Nanoreactors via encapsulation of catalytic gold nanoparticles within cowpea chlorotic mottle virus protein cages. in Methods in Molecular Biology. vol. 1798, Methods in Molecular Biology, vol. 1798, Humana Press Inc., pp. 1-9. https://doi.org/10.1007/978-1-4939-7893-9_1

Nanoreactors via encapsulation of catalytic gold nanoparticles within cowpea chlorotic mottle virus protein cages. / Liu, Aijie; de Ruiter, Mark V.; Maassen, Stan J.; Cornelissen, Jeroen J.L.M.

Methods in Molecular Biology. Vol. 1798 Humana Press Inc., 2018. p. 1-9 (Methods in Molecular Biology; Vol. 1798).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

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Liu A, de Ruiter MV, Maassen SJ, Cornelissen JJLM. Nanoreactors via encapsulation of catalytic gold nanoparticles within cowpea chlorotic mottle virus protein cages. In Methods in Molecular Biology. Vol. 1798. Humana Press Inc. 2018. p. 1-9. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7893-9_1