Nanoscale Electrodes for Bionanosensing

Research output: ThesisPhD Thesis - Research UT, graduation UTAcademic

Abstract

Cancer is globally the second most common cause of death. Cancer burden rises to about 10 million deaths and more than 18 million new cases in 2018. Cancers are often diagnosed at a later stage preventing curative treatment. This underscores the need for an early stage diagnosis of cancer. Consequently, screening methods that can test patients’ samples taken by less invasive methods capable of early stage diagnosis are highly sought for. Based on this motivation, here we developed lab-on-a-chip diagnostic systems that can be used for early detection of cancer. Three different types of nanoscale electrodes were fabricated: (i) nanogap electrodes (ii) nano interdigitated electrodes and (iii) nanodisc electrodes and the possibility of using them for sensing and signal transduction were investigated. Chapter 2 describes the fabrication of nanogap device using conventional optical lithography and DNA detection across it using the electrical method. Chapter 3 details the fabrication of nano interdigitated electrodes (nIDEs) and their electrochemical validation. Chapter 4 describes the biosensing application of nIDEs using nanoparticle sandwich assay for the detection of DNA molecules. Chapter 5 describes the capturing of tdEVs on nIDEs, and its quantification using a sandwich immunosorbent assay on nIDEs. Chapter 6 proposes a new type of nanoscale electrodes which are termed as nanodisc electrodes. Chapter 7 explores the possibility of developing the nanodisc technology to a business idea. In short, the whole thesis tries to explore the different possibilities in developing a sensor that can be useful for cancer diagnosis.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • University of Twente
Supervisors/Advisors
  • van der Wiel, Wilfred Gerard, Supervisor
Award date18 Apr 2019
Place of PublicationEnschede
Publisher
Print ISBNs978-90-365-4764-2
DOIs
Publication statusPublished - 11 Apr 2019

Fingerprint

Electrodes
Assays
Fabrication
Lab-on-a-chip
Signal transduction
Immunosorbents
DNA
Photolithography
Screening
Nanoparticles
Molecules
Sensors
Industry

Keywords

  • nanosensors
  • nanodevices
  • tumor sensor
  • Electrochemical detection

Cite this

Dilu George Mathew, Dilu . / Nanoscale Electrodes for Bionanosensing. Enschede : University of Twente, 2019. 188 p.
@phdthesis{b2e3ff4e29d6450aaf965715a66d22df,
title = "Nanoscale Electrodes for Bionanosensing",
abstract = "Cancer is globally the second most common cause of death. Cancer burden rises to about 10 million deaths and more than 18 million new cases in 2018. Cancers are often diagnosed at a later stage preventing curative treatment. This underscores the need for an early stage diagnosis of cancer. Consequently, screening methods that can test patients’ samples taken by less invasive methods capable of early stage diagnosis are highly sought for. Based on this motivation, here we developed lab-on-a-chip diagnostic systems that can be used for early detection of cancer. Three different types of nanoscale electrodes were fabricated: (i) nanogap electrodes (ii) nano interdigitated electrodes and (iii) nanodisc electrodes and the possibility of using them for sensing and signal transduction were investigated. Chapter 2 describes the fabrication of nanogap device using conventional optical lithography and DNA detection across it using the electrical method. Chapter 3 details the fabrication of nano interdigitated electrodes (nIDEs) and their electrochemical validation. Chapter 4 describes the biosensing application of nIDEs using nanoparticle sandwich assay for the detection of DNA molecules. Chapter 5 describes the capturing of tdEVs on nIDEs, and its quantification using a sandwich immunosorbent assay on nIDEs. Chapter 6 proposes a new type of nanoscale electrodes which are termed as nanodisc electrodes. Chapter 7 explores the possibility of developing the nanodisc technology to a business idea. In short, the whole thesis tries to explore the different possibilities in developing a sensor that can be useful for cancer diagnosis.",
keywords = "nanosensors, nanodevices, tumor sensor, Electrochemical detection",
author = "{Dilu George Mathew}, Dilu",
year = "2019",
month = "4",
day = "11",
doi = "10.3990/1.9789036547642",
language = "English",
isbn = "978-90-365-4764-2",
publisher = "University of Twente",
address = "Netherlands",
school = "University of Twente",

}

Nanoscale Electrodes for Bionanosensing. / Dilu George Mathew, Dilu .

Enschede : University of Twente, 2019. 188 p.

Research output: ThesisPhD Thesis - Research UT, graduation UTAcademic

TY - THES

T1 - Nanoscale Electrodes for Bionanosensing

AU - Dilu George Mathew, Dilu

PY - 2019/4/11

Y1 - 2019/4/11

N2 - Cancer is globally the second most common cause of death. Cancer burden rises to about 10 million deaths and more than 18 million new cases in 2018. Cancers are often diagnosed at a later stage preventing curative treatment. This underscores the need for an early stage diagnosis of cancer. Consequently, screening methods that can test patients’ samples taken by less invasive methods capable of early stage diagnosis are highly sought for. Based on this motivation, here we developed lab-on-a-chip diagnostic systems that can be used for early detection of cancer. Three different types of nanoscale electrodes were fabricated: (i) nanogap electrodes (ii) nano interdigitated electrodes and (iii) nanodisc electrodes and the possibility of using them for sensing and signal transduction were investigated. Chapter 2 describes the fabrication of nanogap device using conventional optical lithography and DNA detection across it using the electrical method. Chapter 3 details the fabrication of nano interdigitated electrodes (nIDEs) and their electrochemical validation. Chapter 4 describes the biosensing application of nIDEs using nanoparticle sandwich assay for the detection of DNA molecules. Chapter 5 describes the capturing of tdEVs on nIDEs, and its quantification using a sandwich immunosorbent assay on nIDEs. Chapter 6 proposes a new type of nanoscale electrodes which are termed as nanodisc electrodes. Chapter 7 explores the possibility of developing the nanodisc technology to a business idea. In short, the whole thesis tries to explore the different possibilities in developing a sensor that can be useful for cancer diagnosis.

AB - Cancer is globally the second most common cause of death. Cancer burden rises to about 10 million deaths and more than 18 million new cases in 2018. Cancers are often diagnosed at a later stage preventing curative treatment. This underscores the need for an early stage diagnosis of cancer. Consequently, screening methods that can test patients’ samples taken by less invasive methods capable of early stage diagnosis are highly sought for. Based on this motivation, here we developed lab-on-a-chip diagnostic systems that can be used for early detection of cancer. Three different types of nanoscale electrodes were fabricated: (i) nanogap electrodes (ii) nano interdigitated electrodes and (iii) nanodisc electrodes and the possibility of using them for sensing and signal transduction were investigated. Chapter 2 describes the fabrication of nanogap device using conventional optical lithography and DNA detection across it using the electrical method. Chapter 3 details the fabrication of nano interdigitated electrodes (nIDEs) and their electrochemical validation. Chapter 4 describes the biosensing application of nIDEs using nanoparticle sandwich assay for the detection of DNA molecules. Chapter 5 describes the capturing of tdEVs on nIDEs, and its quantification using a sandwich immunosorbent assay on nIDEs. Chapter 6 proposes a new type of nanoscale electrodes which are termed as nanodisc electrodes. Chapter 7 explores the possibility of developing the nanodisc technology to a business idea. In short, the whole thesis tries to explore the different possibilities in developing a sensor that can be useful for cancer diagnosis.

KW - nanosensors

KW - nanodevices

KW - tumor sensor

KW - Electrochemical detection

U2 - 10.3990/1.9789036547642

DO - 10.3990/1.9789036547642

M3 - PhD Thesis - Research UT, graduation UT

SN - 978-90-365-4764-2

PB - University of Twente

CY - Enschede

ER -