Negatively charged phospholipids restore prePhoE translocation across phosphatidylglyceroldepleted Escherichia coli inner membranes

Ron Kusters*, William Dowhan, Ben De Kruijff

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

137 Citations (Scopus)
72 Downloads (Pure)

Abstract

Translocation of outer membrane precursor proteins across the Escherichia coli inner membrane is severely hampered in lipid biosynthetic mutants with strongly reduced phosphatidylglycerol (PG) levels (De Vrije, T., De Swart, R. L., Dowhan, W., Tommassen, J., and De Kruijff, B. (1988) Nature 334, 173-175; Lill, R., Dowhan, W., and Wickner, W. (1990) Cell 60, 271-280). Two independent methods were used to demonstrate that anionic lipids by virtue of their negative head-group charge are involved in membrane translocation of the precursor of the pore protein PhoE. Using a lipid transfer protein-based method we show that introduction from lipid vesicles of PG and other acidic phospholipids but not of phosphatidylcholine restores efficient translocation across the membrane of PG-depleted inner membrane vesicles. Moreover, translocation was found to be proportional to the PG content in vesicles isolated from strain HDL11 in which the PG content was altered by varying the synthesis of the PG-phosphate synthase.

Original languageEnglish
Pages (from-to)8659-8662
Number of pages4
JournalJournal of biological chemistry
Volume266
Issue number14
Publication statusPublished - 1 Dec 1991
Externally publishedYes

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