Negatively charged phospholipids restore prePhoE translocation across phosphatidylglyceroldepleted Escherichia coli inner membranes

Ron Kusters*, William Dowhan, Ben De Kruijff

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    127 Citations (Scopus)
    24 Downloads (Pure)

    Abstract

    Translocation of outer membrane precursor proteins across the Escherichia coli inner membrane is severely hampered in lipid biosynthetic mutants with strongly reduced phosphatidylglycerol (PG) levels (De Vrije, T., De Swart, R. L., Dowhan, W., Tommassen, J., and De Kruijff, B. (1988) Nature 334, 173-175; Lill, R., Dowhan, W., and Wickner, W. (1990) Cell 60, 271-280). Two independent methods were used to demonstrate that anionic lipids by virtue of their negative head-group charge are involved in membrane translocation of the precursor of the pore protein PhoE. Using a lipid transfer protein-based method we show that introduction from lipid vesicles of PG and other acidic phospholipids but not of phosphatidylcholine restores efficient translocation across the membrane of PG-depleted inner membrane vesicles. Moreover, translocation was found to be proportional to the PG content in vesicles isolated from strain HDL11 in which the PG content was altered by varying the synthesis of the PG-phosphate synthase.

    Original languageEnglish
    Pages (from-to)8659-8662
    Number of pages4
    JournalJournal of biological chemistry
    Volume266
    Issue number14
    Publication statusPublished - 1 Dec 1991

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