New approach to test growth behaviour of cells for tissue engineered collagenous scaffolds

P. Buijtenhuijs*, L. Buttafoco, A. A. Poot, J. Feijen, I. Vermes

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    Abstract

    A technique developed to characterize and compare the cell growth of human vascular smooth muscle cells (SMC) cultured on tissue-engineered (TE) scaffolds composed of elastin and collagen was discussed. Proliferation and apoptosis of SMC were determined by measuring cyclin E and tissue trans glutaminase (tTG) mRNA expression levels respectively. It was found that with the method SMC cultured on porous films of elastin and collagen grow better than on gelatin-coated tissue culture polystyrene (g-TCPS). The results indicated the possibility to culture cells in a standardized way not only for obtaining an artificial media of TE blood vessel using SMC but also for all kinds of tissue-engineering purposes using other cell types.

    Original languageEnglish
    Title of host publicationTransactions - 7th World Biomaterials Congress
    Pages691
    Number of pages1
    Publication statusPublished - 1 Dec 2004
    Event7th World Biomaterials Congress, WBC 2004 - Sydney, Australia
    Duration: 17 May 200421 May 2004
    Conference number: 7

    Conference

    Conference7th World Biomaterials Congress, WBC 2004
    Abbreviated titleWBC
    CountryAustralia
    CitySydney
    Period17/05/0421/05/04

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