New approach to test growth behaviour of cells for tissue engineered collagenous scaffolds

P. Buijtenhuijs; L. Buttafoco; A. A. Poot; J. Feijen; I. Vermes

New approach to test growth behaviour of cells for tissue engineered collagenous scaffolds

P. Buijtenhuijs^*, L. Buttafoco, A. A. Poot, J. Feijen, I. Vermes

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

A technique developed to characterize and compare the cell growth of human vascular smooth muscle cells (SMC) cultured on tissue-engineered (TE) scaffolds composed of elastin and collagen was discussed. Proliferation and apoptosis of SMC were determined by measuring cyclin E and tissue trans glutaminase (tTG) mRNA expression levels respectively. It was found that with the method SMC cultured on porous films of elastin and collagen grow better than on gelatin-coated tissue culture polystyrene (g-TCPS). The results indicated the possibility to culture cells in a standardized way not only for obtaining an artificial media of TE blood vessel using SMC but also for all kinds of tissue-engineering purposes using other cell types.

Original language	English
Title of host publication	Transactions - 7th World Biomaterials Congress
Pages	691
Number of pages	1
Publication status	Published - 1 Dec 2004
Event	7th World Biomaterials Congress, WBC 2004 - Sydney, Australia Duration: 17 May 2004 → 21 May 2004 Conference number: 7

Conference

Conference	7th World Biomaterials Congress, WBC 2004
Abbreviated title	WBC
Country/Territory	Australia
City	Sydney
Period	17/05/04 → 21/05/04

Cite this

@inproceedings{ce8d37e306c049a5899d2404b1ad14e2,

title = "New approach to test growth behaviour of cells for tissue engineered collagenous scaffolds",

abstract = "A technique developed to characterize and compare the cell growth of human vascular smooth muscle cells (SMC) cultured on tissue-engineered (TE) scaffolds composed of elastin and collagen was discussed. Proliferation and apoptosis of SMC were determined by measuring cyclin E and tissue trans glutaminase (tTG) mRNA expression levels respectively. It was found that with the method SMC cultured on porous films of elastin and collagen grow better than on gelatin-coated tissue culture polystyrene (g-TCPS). The results indicated the possibility to culture cells in a standardized way not only for obtaining an artificial media of TE blood vessel using SMC but also for all kinds of tissue-engineering purposes using other cell types.",

author = "P. Buijtenhuijs and L. Buttafoco and Poot, {A. A.} and J. Feijen and I. Vermes",

year = "2004",

month = dec,

day = "1",

language = "English",

isbn = "1877040193",

pages = "691",

booktitle = "Transactions - 7th World Biomaterials Congress",

note = "7th World Biomaterials Congress, WBC 2004, WBC ; Conference date: 17-05-2004 Through 21-05-2004",

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TY - GEN

T1 - New approach to test growth behaviour of cells for tissue engineered collagenous scaffolds

AU - Buijtenhuijs, P.

AU - Buttafoco, L.

AU - Poot, A. A.

AU - Feijen, J.

AU - Vermes, I.

N1 - Conference code: 7

PY - 2004/12/1

Y1 - 2004/12/1

N2 - A technique developed to characterize and compare the cell growth of human vascular smooth muscle cells (SMC) cultured on tissue-engineered (TE) scaffolds composed of elastin and collagen was discussed. Proliferation and apoptosis of SMC were determined by measuring cyclin E and tissue trans glutaminase (tTG) mRNA expression levels respectively. It was found that with the method SMC cultured on porous films of elastin and collagen grow better than on gelatin-coated tissue culture polystyrene (g-TCPS). The results indicated the possibility to culture cells in a standardized way not only for obtaining an artificial media of TE blood vessel using SMC but also for all kinds of tissue-engineering purposes using other cell types.

AB - A technique developed to characterize and compare the cell growth of human vascular smooth muscle cells (SMC) cultured on tissue-engineered (TE) scaffolds composed of elastin and collagen was discussed. Proliferation and apoptosis of SMC were determined by measuring cyclin E and tissue trans glutaminase (tTG) mRNA expression levels respectively. It was found that with the method SMC cultured on porous films of elastin and collagen grow better than on gelatin-coated tissue culture polystyrene (g-TCPS). The results indicated the possibility to culture cells in a standardized way not only for obtaining an artificial media of TE blood vessel using SMC but also for all kinds of tissue-engineering purposes using other cell types.

UR - http://www.scopus.com/inward/record.url?scp=13844254357&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:13844254357

SN - 1877040193

SN - 9781877040191

SP - 691

BT - Transactions - 7th World Biomaterials Congress

T2 - 7th World Biomaterials Congress, WBC 2004

Y2 - 17 May 2004 through 21 May 2004

ER -

New approach to test growth behaviour of cells for tissue engineered collagenous scaffolds

Abstract

Conference

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