New Technique for High Resolution DNA Sizing in Epi-Illumination

J.M. Schins, A. Agronskaia, B.G. de Grooth, J. Greve

    Research output: Contribution to journalArticleAcademicpeer-review

    8 Citations (Scopus)

    Abstract

    We present a high-resolution DNA-sizing technique based on the principles of flow cytometry, using a high numerical aperture objective and epi-illumination. The new technique, designed for small fluorescing samples/particles (sub-micron diameter) suspended in a weakly fluorescent medium, makes use of an additional focus for high-precision particle localisation. This way, only those particles are considered that flow exactly through a well-defined volume. Results are presented for fluorescent beads, as well as for YOYO-stained plasmids containing 5,500 basepairs. The latter were measured with 6.2% resolution, setting a new limit to flow-based sizing of DNA.
    Original languageEnglish
    Pages (from-to)132-136
    Number of pages5
    JournalCytometry
    Volume32
    Issue number2
    DOIs
    Publication statusPublished - 1998

    Keywords

    • Particle trajectory
    • High numerical aperture
    • DNA-sizing
    • Additional focus

    Fingerprint

    Dive into the research topics of 'New Technique for High Resolution DNA Sizing in Epi-Illumination'. Together they form a unique fingerprint.

    Cite this