NMR of alpha-synuclein-polyamine complexes elucidates the mechanism and kinetics of induced aggregation

Claudio O. Fernández, Wolfgang Hoyer, Markus Zweckstetter, Elizabeth A. Jares-Erijman, Vinod Subramaniam, Christian Griesinger, Thomas M. Jovin

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Abstract

The aggregation of α-synuclein is characteristic of Parkinson's disease (PD) and other neurodegenerative synucleinopathies. The 140-aa protein is natively unstructured; thus, ligands binding to the monomeric form are of therapeutic interest. Biogenic polyamines promote the aggregation of α-synuclein and may constitute endogenous agents modulating the pathogenesis of PD. We characterized the complexes of natural and synthetic polyamines with α-synuclein by NMR and assigned the binding site to C-terminal residues 109–140. Dissociation constants were derived from chemical shift perturbations. Greater polyamine charge (+2 → +5) correlated with increased affinity and enhancement of fibrillation, for which we propose a simple kinetic mechanism involving a dimeric nucleation center. According to the analysis, polyamines increase the extent of nucleation by ~$10^4$ and the rate of monomer addition ~40-fold. Significant secondary structure is not induced in monomeric α-synuclein by polyamines at 15°C. Instead, NMR reveals changes in a region (aa 22–93) far removed from the polyamine binding site and presumed to adopt the β-sheet conformation characteristic of fibrillar α-synuclein. We conclude that the C-terminal domain acts as a regulator of α-synuclein aggregation.
Original languageUndefined
Pages (from-to)1-8
Number of pages8
JournalEMBO journal
Volume23
Issue number10
DOIs
Publication statusPublished - 2004

Keywords

  • METIS-218000
  • IR-80748

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