Non-invasive monitoring of osteogenic differentiation on microtissue arrays under physiological conditions using scanning electrochemical microscopy

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

In this paper, we present a non-invasive assay using scanning electrochemical microscopy (SECM) for detecting osteogenic differentiation at physiological conditions (pH 7.5) on arrays of C2C12 microtissues. Upon exposure to bone morphogenic protein 2 (BMP-2), C2C12 microtissues differentiate and express alkaline phosphatase (ALP), which is indirectly detected through an enzymatic assay producing an electroactive species. The latter is detected using SECM by scanning at constant height over live microtissues at physiological pH (7.5) as well as more alkaline pH (8.5). As a control, expression of ALP is confirmed using a standard colorimetric assay. Detecting differentiation on live samples at physiological conditions represents a significant improvement for continuous monitoring of tissue differentiation or further use of the microtissues for, e.g., regenerative medicine.
Original languageUndefined
Pages (from-to)1881-1885
Number of pages5
JournalElectroanalysis
Volume26
Issue number9
DOIs
Publication statusPublished - Sep 2014

Keywords

  • EWI-25472
  • IR-93335
  • METIS-309757

Cite this

@article{bd4097e6349b427e983070f7f5cc3eaf,
title = "Non-invasive monitoring of osteogenic differentiation on microtissue arrays under physiological conditions using scanning electrochemical microscopy",
abstract = "In this paper, we present a non-invasive assay using scanning electrochemical microscopy (SECM) for detecting osteogenic differentiation at physiological conditions (pH 7.5) on arrays of C2C12 microtissues. Upon exposure to bone morphogenic protein 2 (BMP-2), C2C12 microtissues differentiate and express alkaline phosphatase (ALP), which is indirectly detected through an enzymatic assay producing an electroactive species. The latter is detected using SECM by scanning at constant height over live microtissues at physiological pH (7.5) as well as more alkaline pH (8.5). As a control, expression of ALP is confirmed using a standard colorimetric assay. Detecting differentiation on live samples at physiological conditions represents a significant improvement for continuous monitoring of tissue differentiation or further use of the microtissues for, e.g., regenerative medicine.",
keywords = "EWI-25472, IR-93335, METIS-309757",
author = "Adithya Sridhar and {van den Berg}, Albert and {le Gac}, Severine",
note = "eemcs-eprint-25472",
year = "2014",
month = "9",
doi = "10.1002/elan.201400229",
language = "Undefined",
volume = "26",
pages = "1881--1885",
journal = "Electroanalysis",
issn = "1040-0397",
publisher = "Wiley-VCH Verlag",
number = "9",

}

Non-invasive monitoring of osteogenic differentiation on microtissue arrays under physiological conditions using scanning electrochemical microscopy. / Sridhar, Adithya; van den Berg, Albert; le Gac, Severine.

In: Electroanalysis, Vol. 26, No. 9, 09.2014, p. 1881-1885.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Non-invasive monitoring of osteogenic differentiation on microtissue arrays under physiological conditions using scanning electrochemical microscopy

AU - Sridhar, Adithya

AU - van den Berg, Albert

AU - le Gac, Severine

N1 - eemcs-eprint-25472

PY - 2014/9

Y1 - 2014/9

N2 - In this paper, we present a non-invasive assay using scanning electrochemical microscopy (SECM) for detecting osteogenic differentiation at physiological conditions (pH 7.5) on arrays of C2C12 microtissues. Upon exposure to bone morphogenic protein 2 (BMP-2), C2C12 microtissues differentiate and express alkaline phosphatase (ALP), which is indirectly detected through an enzymatic assay producing an electroactive species. The latter is detected using SECM by scanning at constant height over live microtissues at physiological pH (7.5) as well as more alkaline pH (8.5). As a control, expression of ALP is confirmed using a standard colorimetric assay. Detecting differentiation on live samples at physiological conditions represents a significant improvement for continuous monitoring of tissue differentiation or further use of the microtissues for, e.g., regenerative medicine.

AB - In this paper, we present a non-invasive assay using scanning electrochemical microscopy (SECM) for detecting osteogenic differentiation at physiological conditions (pH 7.5) on arrays of C2C12 microtissues. Upon exposure to bone morphogenic protein 2 (BMP-2), C2C12 microtissues differentiate and express alkaline phosphatase (ALP), which is indirectly detected through an enzymatic assay producing an electroactive species. The latter is detected using SECM by scanning at constant height over live microtissues at physiological pH (7.5) as well as more alkaline pH (8.5). As a control, expression of ALP is confirmed using a standard colorimetric assay. Detecting differentiation on live samples at physiological conditions represents a significant improvement for continuous monitoring of tissue differentiation or further use of the microtissues for, e.g., regenerative medicine.

KW - EWI-25472

KW - IR-93335

KW - METIS-309757

U2 - 10.1002/elan.201400229

DO - 10.1002/elan.201400229

M3 - Article

VL - 26

SP - 1881

EP - 1885

JO - Electroanalysis

JF - Electroanalysis

SN - 1040-0397

IS - 9

ER -