Non-invasive sensing of transepithelial barrier function and tissue differentiation in organs-on-chips using impedance spectroscopy

Marinke W. van der Helm, Olivier Y.F. Henry, Amir Bein, Tiama Hamkins-Indik, Michael J. Cronce, William D. Leineweber, Mathieu Odijk, Andries D. van der Meer, Jan C.T. Eijkel, Donald E. Ingber, Albert van den Berg, Loes I. Segerink (Corresponding Author)

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Abstract

Here, we describe methods for combining impedance spectroscopy measurements with electrical simulation to reveal transepithelial barrier function and tissue structure of human intestinal epithelium cultured inside an organ-on-chip microfluidic culture device. When performing impedance spectroscopy measurements, electrical simulation enabled normalization of cell layer resistance of epithelium cultured statically in a gut-on-a-chip, which enabled determination of transepithelial electrical resistance (TEER) values that can be compared across device platforms. During culture under dynamic flow, the formation of intestinal villi was accompanied by characteristic changes in impedance spectra both measured experimentally and verified with simulation, and we demonstrate that changes in cell layer capacitance may serve as measures of villi differentiation. This method for combining impedance spectroscopy with simulation can be adapted to better monitor cell layer characteristics within any organ-on-chip in vitro and to enable direct quantitative TEER comparisons between organ-on-chip platforms which should help to advance research on organ function.

Original languageEnglish
Pages (from-to)452-463
Number of pages12
JournalLab on a chip
Volume19
Issue number3
DOIs
Publication statusPublished - 29 Jan 2019

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Dielectric Spectroscopy
Electric Impedance
Acoustic impedance
Cells
Spectroscopy
Lab-On-A-Chip Devices
Tissue
Intestinal Mucosa
Microfluidics
Capacitance
Epithelium
Equipment and Supplies
Research

Cite this

van der Helm, Marinke W. ; Henry, Olivier Y.F. ; Bein, Amir ; Hamkins-Indik, Tiama ; Cronce, Michael J. ; Leineweber, William D. ; Odijk, Mathieu ; van der Meer, Andries D. ; Eijkel, Jan C.T. ; Ingber, Donald E. ; van den Berg, Albert ; Segerink, Loes I. / Non-invasive sensing of transepithelial barrier function and tissue differentiation in organs-on-chips using impedance spectroscopy. In: Lab on a chip. 2019 ; Vol. 19, No. 3. pp. 452-463.
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Non-invasive sensing of transepithelial barrier function and tissue differentiation in organs-on-chips using impedance spectroscopy. / van der Helm, Marinke W.; Henry, Olivier Y.F.; Bein, Amir; Hamkins-Indik, Tiama; Cronce, Michael J.; Leineweber, William D.; Odijk, Mathieu; van der Meer, Andries D.; Eijkel, Jan C.T.; Ingber, Donald E.; van den Berg, Albert; Segerink, Loes I. (Corresponding Author).

In: Lab on a chip, Vol. 19, No. 3, 29.01.2019, p. 452-463.

Research output: Contribution to journalArticleAcademicpeer-review

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