Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties

Martin Piest, J. Feijen (Editor), W.E. Hennink (Editor), C. Lin, Miguel Mateos timoneda, A.P. Sam (Editor), M.C. Lok, Jan Feijen, Johannes F.J. Engbersen

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A series of novel water-soluble, bioreducible poly(amido amine)s (SS-PAAs) was synthesized by Michael addition polymerization of N,N′-dimethylcystamine (DMC) with various bisacrylamides. DMC-based SS-PAAs show high buffer capacities in the pH range 5.1–7.4, facilitating endosomal escape. The polymers form nanoscaled (< 250 nm) and positively charged (> + 20 mV) polyplexes which are relatively stable, but rapidly disintegrate in the presence of 2.5 mM DTT. These polyplexes have transfection efficiencies up to 4 times higher than bPEI in COS-7 cells, with low cytotoxicity.
Original languageUndefined
Publication statusPublished - 2 Apr 2008
Event10th European Symposium on Controlled Drug Delivery, ESCDD 2008 - Noordwijk aan Zee, Netherlands
Duration: 2 Apr 20084 Apr 2008
Conference number: 10


Conference10th European Symposium on Controlled Drug Delivery, ESCDD 2008
Abbreviated titleESCDD
CityNoordwijk aan Zee


  • METIS-254629
  • IR-69320

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