A series of novel water-soluble, bioreducible poly(amido amine)s (SS-PAAs) was synthesized by Michael addition polymerization of N,N′-dimethylcystamine (DMC) with various bisacrylamides. DMC-based SS-PAAs show high buffer capacities in the pH range 5.1–7.4, facilitating endosomal escape. The polymers form nanoscaled (< 250 nm) and positively charged (> + 20 mV) polyplexes which are relatively stable, but rapidly disintegrate in the presence of 2.5 mM DTT. These polyplexes have transfection efficiencies up to 4 times higher than bPEI in COS-7 cells, with low cytotoxicity.
|Publication status||Published - 2 Apr 2008|
|Event||10th European Symposium on Controlled Drug Delivery, ESCDD 2008 - Noordwijk aan Zee, Netherlands|
Duration: 2 Apr 2008 → 4 Apr 2008
Conference number: 10
|Conference||10th European Symposium on Controlled Drug Delivery, ESCDD 2008|
|City||Noordwijk aan Zee|
|Period||2/04/08 → 4/04/08|