Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties

Martin Piest, J. Feijen (Editor), W.E. Hennink (Editor), C. Lin, Miguel Mateos timoneda, A.P. Sam (Editor), M.C. Lok, Jan Feijen, Johannes F.J. Engbersen

Research output: Contribution to conferencePaperAcademic

70 Citations (Scopus)

Abstract

A series of novel water-soluble, bioreducible poly(amido amine)s (SS-PAAs) was synthesized by Michael addition polymerization of N,N′-dimethylcystamine (DMC) with various bisacrylamides. DMC-based SS-PAAs show high buffer capacities in the pH range 5.1–7.4, facilitating endosomal escape. The polymers form nanoscaled (< 250 nm) and positively charged (> + 20 mV) polyplexes which are relatively stable, but rapidly disintegrate in the presence of 2.5 mM DTT. These polyplexes have transfection efficiencies up to 4 times higher than bPEI in COS-7 cells, with low cytotoxicity.
Original languageUndefined
Pagese12-e13
DOIs
Publication statusPublished - 2 Apr 2008
Event10th European Symposium on Controlled Drug Delivery, ESCDD 2008 - Noordwijk aan Zee, Netherlands
Duration: 2 Apr 20084 Apr 2008
Conference number: 10

Conference

Conference10th European Symposium on Controlled Drug Delivery, ESCDD 2008
Abbreviated titleESCDD
CountryNetherlands
CityNoordwijk aan Zee
Period2/04/084/04/08

Keywords

  • METIS-254629
  • IR-69320

Cite this

Piest, M., Feijen, J. (Ed.), Hennink, W. E. (Ed.), Lin, C., Mateos timoneda, M., Sam, A. P. (Ed.), ... Engbersen, J. F. J. (2008). Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties. e12-e13. Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands. https://doi.org/10.1016/j.jconrel.2008.09.047
Piest, Martin ; Feijen, J. (Editor) ; Hennink, W.E. (Editor) ; Lin, C. ; Mateos timoneda, Miguel ; Sam, A.P. (Editor) ; Lok, M.C. ; Feijen, Jan ; Engbersen, Johannes F.J. / Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties. Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands.
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title = "Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties",
abstract = "A series of novel water-soluble, bioreducible poly(amido amine)s (SS-PAAs) was synthesized by Michael addition polymerization of N,N′-dimethylcystamine (DMC) with various bisacrylamides. DMC-based SS-PAAs show high buffer capacities in the pH range 5.1–7.4, facilitating endosomal escape. The polymers form nanoscaled (< 250 nm) and positively charged (> + 20 mV) polyplexes which are relatively stable, but rapidly disintegrate in the presence of 2.5 mM DTT. These polyplexes have transfection efficiencies up to 4 times higher than bPEI in COS-7 cells, with low cytotoxicity.",
keywords = "METIS-254629, IR-69320",
author = "Martin Piest and J. Feijen and W.E. Hennink and C. Lin and {Mateos timoneda}, Miguel and A.P. Sam and M.C. Lok and Jan Feijen and Engbersen, {Johannes F.J.}",
note = "Proceedings of the Tenth European Symposium on Controlled Drug Delivery ; null ; Conference date: 02-04-2008 Through 04-04-2008",
year = "2008",
month = "4",
day = "2",
doi = "10.1016/j.jconrel.2008.09.047",
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Piest, M, Feijen, J (ed.), Hennink, WE (ed.), Lin, C, Mateos timoneda, M, Sam, AP (ed.), Lok, MC, Feijen, J & Engbersen, JFJ 2008, 'Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties' Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands, 2/04/08 - 4/04/08, pp. e12-e13. https://doi.org/10.1016/j.jconrel.2008.09.047

Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties. / Piest, Martin; Feijen, J. (Editor); Hennink, W.E. (Editor); Lin, C.; Mateos timoneda, Miguel; Sam, A.P. (Editor); Lok, M.C.; Feijen, Jan; Engbersen, Johannes F.J.

2008. e12-e13 Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands.

Research output: Contribution to conferencePaperAcademic

TY - CONF

T1 - Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties

AU - Piest, Martin

AU - Lin, C.

AU - Mateos timoneda, Miguel

AU - Lok, M.C.

AU - Feijen, Jan

AU - Engbersen, Johannes F.J.

A2 - Feijen, J.

A2 - Hennink, W.E.

A2 - Sam, A.P.

N1 - Proceedings of the Tenth European Symposium on Controlled Drug Delivery

PY - 2008/4/2

Y1 - 2008/4/2

N2 - A series of novel water-soluble, bioreducible poly(amido amine)s (SS-PAAs) was synthesized by Michael addition polymerization of N,N′-dimethylcystamine (DMC) with various bisacrylamides. DMC-based SS-PAAs show high buffer capacities in the pH range 5.1–7.4, facilitating endosomal escape. The polymers form nanoscaled (< 250 nm) and positively charged (> + 20 mV) polyplexes which are relatively stable, but rapidly disintegrate in the presence of 2.5 mM DTT. These polyplexes have transfection efficiencies up to 4 times higher than bPEI in COS-7 cells, with low cytotoxicity.

AB - A series of novel water-soluble, bioreducible poly(amido amine)s (SS-PAAs) was synthesized by Michael addition polymerization of N,N′-dimethylcystamine (DMC) with various bisacrylamides. DMC-based SS-PAAs show high buffer capacities in the pH range 5.1–7.4, facilitating endosomal escape. The polymers form nanoscaled (< 250 nm) and positively charged (> + 20 mV) polyplexes which are relatively stable, but rapidly disintegrate in the presence of 2.5 mM DTT. These polyplexes have transfection efficiencies up to 4 times higher than bPEI in COS-7 cells, with low cytotoxicity.

KW - METIS-254629

KW - IR-69320

U2 - 10.1016/j.jconrel.2008.09.047

DO - 10.1016/j.jconrel.2008.09.047

M3 - Paper

SP - e12-e13

ER -

Piest M, Feijen J, (ed.), Hennink WE, (ed.), Lin C, Mateos timoneda M, Sam AP, (ed.) et al. Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties. 2008. Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands. https://doi.org/10.1016/j.jconrel.2008.09.047