Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties

Martin Piest; J. Feijen; W.E. Hennink; C. Lin; Miguel Mateos timoneda; A.P. Sam; M.C. Lok; Jan Feijen; Johannes F.J. Engbersen

doi:10.1016/j.jconrel.2008.09.047

Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties

Martin Piest, J. Feijen (Editor), W.E. Hennink (Editor), C. Lin, Miguel Mateos timoneda, A.P. Sam (Editor), M.C. Lok, Jan Feijen, Johannes F.J. Engbersen

Research output: Contribution to conference › Paper

79 Citations (Scopus)

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Abstract

A series of novel water-soluble, bioreducible poly(amido amine)s (SS-PAAs) was synthesized by Michael addition polymerization of N,N′-dimethylcystamine (DMC) with various bisacrylamides. DMC-based SS-PAAs show high buffer capacities in the pH range 5.1–7.4, facilitating endosomal escape. The polymers form nanoscaled (< 250 nm) and positively charged (> + 20 mV) polyplexes which are relatively stable, but rapidly disintegrate in the presence of 2.5 mM DTT. These polyplexes have transfection efficiencies up to 4 times higher than bPEI in COS-7 cells, with low cytotoxicity.

Original language	Undefined
Pages	e12-e13
DOIs	https://doi.org/10.1016/j.jconrel.2008.09.047
Publication status	Published - 2 Apr 2008
Event	10th European Symposium on Controlled Drug Delivery, ESCDD 2008 - Noordwijk aan Zee, Netherlands Duration: 2 Apr 2008 → 4 Apr 2008 Conference number: 10

Conference

Conference	10th European Symposium on Controlled Drug Delivery, ESCDD 2008
Abbreviated title	ESCDD
Country/Territory	Netherlands
City	Noordwijk aan Zee
Period	2/04/08 → 4/04/08

Keywords

METIS-254629
IR-69320

Access to Document

10.1016/j.jconrel.2008.09.047

Cite this

Piest, M., Feijen, J. (Ed.), Hennink, W. E. (Ed.), Lin, C., Mateos timoneda, M., Sam, A. P. (Ed.), Lok, M. C., Feijen, J., & Engbersen, J. F. J. (2008). Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties. e12-e13. Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands. https://doi.org/10.1016/j.jconrel.2008.09.047

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title = "Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties",

abstract = "A series of novel water-soluble, bioreducible poly(amido amine)s (SS-PAAs) was synthesized by Michael addition polymerization of N,N′-dimethylcystamine (DMC) with various bisacrylamides. DMC-based SS-PAAs show high buffer capacities in the pH range 5.1–7.4, facilitating endosomal escape. The polymers form nanoscaled (< 250 nm) and positively charged (> + 20 mV) polyplexes which are relatively stable, but rapidly disintegrate in the presence of 2.5 mM DTT. These polyplexes have transfection efficiencies up to 4 times higher than bPEI in COS-7 cells, with low cytotoxicity.",

keywords = "METIS-254629, IR-69320",

author = "Martin Piest and J. Feijen and W.E. Hennink and C. Lin and {Mateos timoneda}, Miguel and A.P. Sam and M.C. Lok and Jan Feijen and Engbersen, {Johannes F.J.}",

note = "Proceedings of the Tenth European Symposium on Controlled Drug Delivery ; 10th European Symposium on Controlled Drug Delivery, ESCDD 2008 ; Conference date: 02-04-2008 Through 04-04-2008",

year = "2008",

month = apr,

day = "2",

doi = "10.1016/j.jconrel.2008.09.047",

language = "Undefined",

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}

Piest, M, Feijen, J (ed.), Hennink, WE (ed.), Lin, C, Mateos timoneda, M, Sam, AP (ed.), Lok, MC, Feijen, J & Engbersen, JFJ 2008, 'Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties', Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands, 2/04/08 - 4/04/08 pp. e12-e13. https://doi.org/10.1016/j.jconrel.2008.09.047

Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties. / Piest, Martin; Feijen, J. (Editor); Hennink, W.E. (Editor) et al.
2008. e12-e13 Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands.

Research output: Contribution to conference › Paper

TY - CONF

T1 - Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties

AU - Piest, Martin

AU - Lin, C.

AU - Mateos timoneda, Miguel

AU - Lok, M.C.

AU - Feijen, Jan

AU - Engbersen, Johannes F.J.

A2 - Feijen, J.

A2 - Hennink, W.E.

A2 - Sam, A.P.

N1 - Conference code: 10

PY - 2008/4/2

Y1 - 2008/4/2

N2 - A series of novel water-soluble, bioreducible poly(amido amine)s (SS-PAAs) was synthesized by Michael addition polymerization of N,N′-dimethylcystamine (DMC) with various bisacrylamides. DMC-based SS-PAAs show high buffer capacities in the pH range 5.1–7.4, facilitating endosomal escape. The polymers form nanoscaled (< 250 nm) and positively charged (> + 20 mV) polyplexes which are relatively stable, but rapidly disintegrate in the presence of 2.5 mM DTT. These polyplexes have transfection efficiencies up to 4 times higher than bPEI in COS-7 cells, with low cytotoxicity.

AB - A series of novel water-soluble, bioreducible poly(amido amine)s (SS-PAAs) was synthesized by Michael addition polymerization of N,N′-dimethylcystamine (DMC) with various bisacrylamides. DMC-based SS-PAAs show high buffer capacities in the pH range 5.1–7.4, facilitating endosomal escape. The polymers form nanoscaled (< 250 nm) and positively charged (> + 20 mV) polyplexes which are relatively stable, but rapidly disintegrate in the presence of 2.5 mM DTT. These polyplexes have transfection efficiencies up to 4 times higher than bPEI in COS-7 cells, with low cytotoxicity.

KW - METIS-254629

KW - IR-69320

U2 - 10.1016/j.jconrel.2008.09.047

DO - 10.1016/j.jconrel.2008.09.047

M3 - Paper

SP - e12-e13

T2 - 10th European Symposium on Controlled Drug Delivery, ESCDD 2008

Y2 - 2 April 2008 through 4 April 2008

ER -

Piest M, Feijen J, (ed.), Hennink WE, (ed.), Lin C, Mateos timoneda M, Sam AP, (ed.) et al.. Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties. 2008. Paper presented at 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands. doi: 10.1016/j.jconrel.2008.09.047