Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties

Martin Piest; Chao Lin; Miguel A. Mateos Timoneda; Martin C. Lok; Jan Feijen; Johan F.J. Engbersen

doi:10.1016/j.jconrel.2008.09.047

Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties

Martin Piest
, Chao Lin
, Miguel A. Mateos Timoneda
, Martin C. Lok
, Jan Feijen
, Johan F.J. Engbersen

Research output: Contribution to conference › Abstract › Academic

79 Citations (Scopus)

2 Downloads (Pure)

Abstract

A series of novel water-soluble, bioreducible poly(amido amine)s (SS-PAAs) was synthesized by Michael addition polymerization of N,N′-dimethylcystamine (DMC) with various bisacrylamides. DMC-based SS-PAAs show high buffer capacities in the pH range 5.1–7.4, facilitating endosomal escape. The polymers form nanoscaled (< 250 nm) and positively charged (> + 20 mV) polyplexes which are relatively stable, but rapidly disintegrate in the presence of 2.5 mM DTT. These polyplexes have transfection efficiencies up to 4 times higher than bPEI in COS-7 cells, with low cytotoxicity.

Original language	English
Pages	e12-e13
DOIs	https://doi.org/10.1016/j.jconrel.2008.09.047
Publication status	Published - 2 Apr 2008
Event	10th European Symposium on Controlled Drug Delivery, ESCDD 2008 - Noordwijk aan Zee, Netherlands Duration: 2 Apr 2008 → 4 Apr 2008 Conference number: 10

Conference

Conference	10th European Symposium on Controlled Drug Delivery, ESCDD 2008
Abbreviated title	ESCDD
Country/Territory	Netherlands
City	Noordwijk aan Zee
Period	2/04/08 → 4/04/08

Access to Document

10.1016/j.jconrel.2008.09.047

79 Citations
1 Paper
1 Article

Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties
Piest, M., Lin, C., Mateos-Timoneda, M. A., Lok, M. C., Hennink, W. E., Feijen, J. & Engbersen, J. F. J., 2008, In: Journal of controlled release. 130, 1, p. 38-45
Research output: Contribution to journal › Article › Academic › peer-review
15 Downloads (Pure)
Novel poly (amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties
Piest, M., Lin, C., Mateos Timoneda, M. A., Lok, M. C., Hennink, W., Feijen, J. & Engbersen, J. F. J., 2 Apr 2008, p. 101-103.
Research output: Contribution to conference › Paper

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Piest, M., Lin, C., Mateos Timoneda, M. A., Lok, M. C., Feijen, J., & Engbersen, J. F. J. (2008). Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties. e12-e13. Abstract from 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands. https://doi.org/10.1016/j.jconrel.2008.09.047

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title = "Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties",

abstract = "A series of novel water-soluble, bioreducible poly(amido amine)s (SS-PAAs) was synthesized by Michael addition polymerization of N,N′-dimethylcystamine (DMC) with various bisacrylamides. DMC-based SS-PAAs show high buffer capacities in the pH range 5.1–7.4, facilitating endosomal escape. The polymers form nanoscaled (< 250 nm) and positively charged (> + 20 mV) polyplexes which are relatively stable, but rapidly disintegrate in the presence of 2.5 mM DTT. These polyplexes have transfection efficiencies up to 4 times higher than bPEI in COS-7 cells, with low cytotoxicity.",

author = "Martin Piest and Chao Lin and \{Mateos Timoneda\}, \{Miguel A.\} and Lok, \{Martin C.\} and Jan Feijen and Engbersen, \{Johan F.J.\}",

note = "Proceedings of the Tenth European Symposium on Controlled Drug Delivery ; 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, ESCDD ; Conference date: 02-04-2008 Through 04-04-2008",

year = "2008",

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doi = "10.1016/j.jconrel.2008.09.047",

language = "English",

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Piest, M, Lin, C, Mateos Timoneda, MA, Lok, MC, Feijen, J & Engbersen, JFJ 2008, 'Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties', 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands, 2/04/08 - 4/04/08 pp. e12-e13. https://doi.org/10.1016/j.jconrel.2008.09.047

Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties. / Piest, Martin; Lin, Chao; Mateos Timoneda, Miguel A. et al.
2008. e12-e13 Abstract from 10th European Symposium on Controlled Drug Delivery, ESCDD 2008, Noordwijk aan Zee, Netherlands.

Research output: Contribution to conference › Abstract › Academic

TY - CONF

T1 - Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties

AU - Piest, Martin

AU - Lin, Chao

AU - Mateos Timoneda, Miguel A.

AU - Lok, Martin C.

AU - Feijen, Jan

AU - Engbersen, Johan F.J.

N1 - Conference code: 10

PY - 2008/4/2

Y1 - 2008/4/2

N2 - A series of novel water-soluble, bioreducible poly(amido amine)s (SS-PAAs) was synthesized by Michael addition polymerization of N,N′-dimethylcystamine (DMC) with various bisacrylamides. DMC-based SS-PAAs show high buffer capacities in the pH range 5.1–7.4, facilitating endosomal escape. The polymers form nanoscaled (< 250 nm) and positively charged (> + 20 mV) polyplexes which are relatively stable, but rapidly disintegrate in the presence of 2.5 mM DTT. These polyplexes have transfection efficiencies up to 4 times higher than bPEI in COS-7 cells, with low cytotoxicity.

AB - A series of novel water-soluble, bioreducible poly(amido amine)s (SS-PAAs) was synthesized by Michael addition polymerization of N,N′-dimethylcystamine (DMC) with various bisacrylamides. DMC-based SS-PAAs show high buffer capacities in the pH range 5.1–7.4, facilitating endosomal escape. The polymers form nanoscaled (< 250 nm) and positively charged (> + 20 mV) polyplexes which are relatively stable, but rapidly disintegrate in the presence of 2.5 mM DTT. These polyplexes have transfection efficiencies up to 4 times higher than bPEI in COS-7 cells, with low cytotoxicity.

U2 - 10.1016/j.jconrel.2008.09.047

DO - 10.1016/j.jconrel.2008.09.047

M3 - Abstract

SP - e12-e13

T2 - 10th European Symposium on Controlled Drug Delivery, ESCDD 2008

Y2 - 2 April 2008 through 4 April 2008

ER -

Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties

Abstract

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Research output

Novel poly(amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties

Novel poly (amido amine)s with bioreducible disulfide linkages in their diamino-units: structure effects and in vitro gene transfer properties

Cite this