Abstract
The increasing frequency of virus-related disease outbreaks creates a growing need for methods that permit rapid detection of infectious biological agents, such as viruses, within small sample volumes and without amplification of the agent. Surface-based biosensors emerge as a promising set of detection devices that are suitable for this purpose. Moreover, recent experimental studies have demonstrated that the detection capabilities of these sensors can be significantly enhanced when their operation is aided by AC electrokinetic effects (dielectrophoretic and AC electrohydrodynamic forces), which can cause accelerated transport and capture of biological agents on microelectrode platforms situated on the sensor's surface. The present study is concerned with the 3D modeling and simulation of the phenomena that govern viral transport to, and capture on, a microelectrode surface inside media of physiological ionic strength under the influence of a spatially non-uniform AC electric field. More specifically, the resulting force patterns (viscous and dielectrophoretic) on particles are assessed by using a finite element method-based simulation package (COMSOL Multiphysics®). The validity of the computer simulations is confirmed through experiments involving electrokinetically-directed capture of fluorescent sub-micron latex particles under the same conditions.
| Original language | English |
|---|---|
| Title of host publication | 2006 AIChE Annual Meeting |
| Number of pages | 8 |
| Publication status | Published - 1 Dec 2006 |
| Externally published | Yes |
| Event | 2006 AIChE Annual Meeting - San Francisco, United States Duration: 12 Nov 2006 → 17 Nov 2006 |
Publication series
| Name | AIChE Annual Meeting, Conference Proceedings |
|---|
Conference
| Conference | 2006 AIChE Annual Meeting |
|---|---|
| Country | United States |
| City | San Francisco |
| Period | 12/11/06 → 17/11/06 |
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Numerical simulations of electrokinetically-driven capture of viral particles inside media of high ionic strength. / Docoslis, Aristides; Zhang, Bingbing; Tomkins, Matthew R.; Wood, Jeffery A.
2006 AIChE Annual Meeting. 2006. (AIChE Annual Meeting, Conference Proceedings).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review
TY - GEN
T1 - Numerical simulations of electrokinetically-driven capture of viral particles inside media of high ionic strength
AU - Docoslis, Aristides
AU - Zhang, Bingbing
AU - Tomkins, Matthew R.
AU - Wood, Jeffery A.
PY - 2006/12/1
Y1 - 2006/12/1
N2 - The increasing frequency of virus-related disease outbreaks creates a growing need for methods that permit rapid detection of infectious biological agents, such as viruses, within small sample volumes and without amplification of the agent. Surface-based biosensors emerge as a promising set of detection devices that are suitable for this purpose. Moreover, recent experimental studies have demonstrated that the detection capabilities of these sensors can be significantly enhanced when their operation is aided by AC electrokinetic effects (dielectrophoretic and AC electrohydrodynamic forces), which can cause accelerated transport and capture of biological agents on microelectrode platforms situated on the sensor's surface. The present study is concerned with the 3D modeling and simulation of the phenomena that govern viral transport to, and capture on, a microelectrode surface inside media of physiological ionic strength under the influence of a spatially non-uniform AC electric field. More specifically, the resulting force patterns (viscous and dielectrophoretic) on particles are assessed by using a finite element method-based simulation package (COMSOL Multiphysics®). The validity of the computer simulations is confirmed through experiments involving electrokinetically-directed capture of fluorescent sub-micron latex particles under the same conditions.
AB - The increasing frequency of virus-related disease outbreaks creates a growing need for methods that permit rapid detection of infectious biological agents, such as viruses, within small sample volumes and without amplification of the agent. Surface-based biosensors emerge as a promising set of detection devices that are suitable for this purpose. Moreover, recent experimental studies have demonstrated that the detection capabilities of these sensors can be significantly enhanced when their operation is aided by AC electrokinetic effects (dielectrophoretic and AC electrohydrodynamic forces), which can cause accelerated transport and capture of biological agents on microelectrode platforms situated on the sensor's surface. The present study is concerned with the 3D modeling and simulation of the phenomena that govern viral transport to, and capture on, a microelectrode surface inside media of physiological ionic strength under the influence of a spatially non-uniform AC electric field. More specifically, the resulting force patterns (viscous and dielectrophoretic) on particles are assessed by using a finite element method-based simulation package (COMSOL Multiphysics®). The validity of the computer simulations is confirmed through experiments involving electrokinetically-directed capture of fluorescent sub-micron latex particles under the same conditions.
UR - http://www.scopus.com/inward/record.url?scp=57049175837&partnerID=8YFLogxK
M3 - Conference contribution
SN - 081691012X
SN - 9780816910120
T3 - AIChE Annual Meeting, Conference Proceedings
BT - 2006 AIChE Annual Meeting
ER -