Ocean Carbon Cycle Studied by Single-cell Impedance Cytometry on Calcifying Algae

Douwe Sietse de Bruijn*, Paulus Martinus ter Braak, Dedmer B. Van de Waal, Wouter Olthuis, Albert van den Berg

*Corresponding author for this work

Research output: Contribution to conferencePaperAcademicpeer-review

Abstract

Algae play an important role in the global carbon cycle. We explore the use of microfluidic single-cell impedance spectroscopy in the field of calcifying algae. The ratio of the impedance at high frequency vs. low frequency, known as opacity, allows us to discriminate between calcified coccolithophores and coccolithophores with a calcite exoskeleton dissolved by acidification (decalcified). In the experimental work we demonstrate that we can discriminate between calcified and decalcified coccolithophores with an accuracy of 94.1% using opacity.

Original languageEnglish
Pages803-804
Number of pages2
Publication statusPublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2020 - Virtual Event
Duration: 4 Oct 20209 Oct 2020
Conference number: 24
https://microtas2020.org/

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2020
Abbreviated titleMicroTAS 2020
CityVirtual Event
Period4/10/209/10/20
Internet address

Keywords

  • Algae
  • Calcification
  • Electrical Impedance Spectroscopy
  • Equivalent Circuit Model
  • Flow Cytometry
  • Single-cell Characterization

Fingerprint Dive into the research topics of 'Ocean Carbon Cycle Studied by Single-cell Impedance Cytometry on Calcifying Algae'. Together they form a unique fingerprint.

Cite this