Ocean Carbon Cycle Studied by Single-cell Impedance Cytometry on Calcifying Algae

Douwe Sietse de Bruijn; Paulus Martinus ter Braak; Dedmer B. Van de Waal; Wouter  Olthuis; Albert  van den Berg

Ocean Carbon Cycle Studied by Single-cell Impedance Cytometry on Calcifying Algae

Douwe Sietse de Bruijn^*, Paulus Martinus ter Braak, Dedmer B. Van de Waal, Wouter Olthuis, Albert van den Berg

^*Corresponding author for this work

Research output: Contribution to conference › Paper › peer-review

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Abstract

Algae play an important role in the global carbon cycle. We explore the use of microfluidic single-cell impedance spectroscopy in the field of calcifying algae. The ratio of the impedance at high frequency vs. low frequency, known as opacity, allows us to discriminate between calcified coccolithophores and coccolithophores with a calcite exoskeleton dissolved by acidification (decalcified). In the experimental work we demonstrate that we can discriminate between calcified and decalcified coccolithophores with an accuracy of 94.1% using opacity.

Original language	English
Pages	803-804
Number of pages	2
Publication status	Published - 2020
Event	24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2020 - Virtual Event Duration: 4 Oct 2020 → 9 Oct 2020 Conference number: 24 https://microtas2020.org/

Conference

Conference	24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2020
Abbreviated title	MicroTAS 2020
City	Virtual Event
Period	4/10/20 → 9/10/20
Internet address	https://microtas2020.org/

Keywords

2021 OA procedure
Calcification
Electrical Impedance Spectroscopy
Equivalent Circuit Model
Flow Cytometry
Single-cell Characterization
Algae

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MicroTAS2020_deBruijnFinal published version, 357 KBLicence: Taverne

Cite this

@conference{860be70399874940bf5cfbec05be8fb1,

title = "Ocean Carbon Cycle Studied by Single-cell Impedance Cytometry on Calcifying Algae",

abstract = "Algae play an important role in the global carbon cycle. We explore the use of microfluidic single-cell impedance spectroscopy in the field of calcifying algae. The ratio of the impedance at high frequency vs. low frequency, known as opacity, allows us to discriminate between calcified coccolithophores and coccolithophores with a calcite exoskeleton dissolved by acidification (decalcified). In the experimental work we demonstrate that we can discriminate between calcified and decalcified coccolithophores with an accuracy of 94.1% using opacity.",

keywords = "2021 OA procedure, Calcification, Electrical Impedance Spectroscopy, Equivalent Circuit Model, Flow Cytometry, Single-cell Characterization, Algae",

author = "{de Bruijn}, {Douwe Sietse} and {ter Braak}, {Paulus Martinus} and {Van de Waal}, {Dedmer B.} and Wouter Olthuis and {van den Berg}, Albert",

year = "2020",

language = "English",

pages = "803--804",

note = "24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2020, MicroTAS 2020 ; Conference date: 04-10-2020 Through 09-10-2020",

url = "https://microtas2020.org/",

}

Ocean Carbon Cycle Studied by Single-cell Impedance Cytometry on Calcifying Algae. / de Bruijn, Douwe Sietse; ter Braak, Paulus Martinus; Van de Waal, Dedmer B. et al.
2020. 803-804 Paper presented at 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2020, Virtual Event.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Ocean Carbon Cycle Studied by Single-cell Impedance Cytometry on Calcifying Algae

AU - de Bruijn, Douwe Sietse

AU - ter Braak, Paulus Martinus

AU - Van de Waal, Dedmer B.

AU - Olthuis, Wouter

AU - van den Berg, Albert

N1 - Conference code: 24

PY - 2020

Y1 - 2020

N2 - Algae play an important role in the global carbon cycle. We explore the use of microfluidic single-cell impedance spectroscopy in the field of calcifying algae. The ratio of the impedance at high frequency vs. low frequency, known as opacity, allows us to discriminate between calcified coccolithophores and coccolithophores with a calcite exoskeleton dissolved by acidification (decalcified). In the experimental work we demonstrate that we can discriminate between calcified and decalcified coccolithophores with an accuracy of 94.1% using opacity.

AB - Algae play an important role in the global carbon cycle. We explore the use of microfluidic single-cell impedance spectroscopy in the field of calcifying algae. The ratio of the impedance at high frequency vs. low frequency, known as opacity, allows us to discriminate between calcified coccolithophores and coccolithophores with a calcite exoskeleton dissolved by acidification (decalcified). In the experimental work we demonstrate that we can discriminate between calcified and decalcified coccolithophores with an accuracy of 94.1% using opacity.

KW - 2021 OA procedure

KW - Calcification

KW - Electrical Impedance Spectroscopy

KW - Equivalent Circuit Model

KW - Flow Cytometry

KW - Single-cell Characterization

KW - Algae

UR - http://www.scopus.com/inward/record.url?scp=85098259886&partnerID=8YFLogxK

M3 - Paper

SP - 803

EP - 804

T2 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2020

Y2 - 4 October 2020 through 9 October 2020

ER -

Ocean Carbon Cycle Studied by Single-cell Impedance Cytometry on Calcifying Algae

Abstract

Conference

Keywords

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