On-chip bulk-index concentration and direct, label-free protein sensing utilizing an optical grated-waveguide cavity

P.V.S. Pham Van So, Mindert Dijkstra, Anton Hollink, L.J. Kauppinen, R.M. de Ridder, Markus Pollnau, Paul Lambeck, Hugo Hoekstra

    Research output: Contribution to journalArticleAcademicpeer-review

    22 Citations (Scopus)

    Abstract

    A theoretical and experimental evaluation is given of a principle of direct, label-free opto-chemical sensing. According to this principle, which is applicable in compact planar optical sensors, measurand-induced wavelength shifts of the sharp fringes in the transmission spectra near the stop band edges of a resonant grating-based cavity are monitored. Such fringes are the results of Fabry–Perot resonances of the Bloch modes propagating in the cavity. Two sensor configurations have been considered, a first one for measuring the concentration of a single compound dissolved in water in the vicinity of the grating (bulk sensing), and a second one for determining the concentration of a specific compound adsorbed at the grating surface from a watery mixture of many compounds (surface sensing). In the latter, a thin interface layer which contains receptors specific to the targeted analyte, the PepN enzyme, is applied on top of the grated waveguide section. Filling of the receptors can be effectively seen as growth of an adlayer. Experimentally resolutions of 6×10−6 refractive index unit and ∼4 pm adlayer growth have been obtained for bulk and surface sensing, respectively. With a statistical analysis the limitations to obtain lower resolutions with the current set-up are identified. The compact devices (footprint ∼200 μm × 15 μm) are well suited for multi-sensing in lab-on-a-chip systems and can easily be fabricated with standard micro-fluidic and CMOS technologies.
    Original languageUndefined
    Pages (from-to)602-608
    Number of pages7
    JournalSensors and actuators. B: Chemical
    Volume174
    DOIs
    Publication statusPublished - 1 Nov 2012

    Keywords

    • EWI-22414
    • IOMS-SNS: SENSORS
    • Grated-waveguide cavity
    • Label-free biosensing
    • Bulk sensing
    • Surface sensing
    • METIS-289750
    • IR-82113
    • Statistical Analysis

    Cite this

    Pham Van So, P.V.S. ; Dijkstra, Mindert ; Hollink, Anton ; Kauppinen, L.J. ; de Ridder, R.M. ; Pollnau, Markus ; Lambeck, Paul ; Hoekstra, Hugo. / On-chip bulk-index concentration and direct, label-free protein sensing utilizing an optical grated-waveguide cavity. In: Sensors and actuators. B: Chemical. 2012 ; Vol. 174. pp. 602-608.
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    title = "On-chip bulk-index concentration and direct, label-free protein sensing utilizing an optical grated-waveguide cavity",
    abstract = "A theoretical and experimental evaluation is given of a principle of direct, label-free opto-chemical sensing. According to this principle, which is applicable in compact planar optical sensors, measurand-induced wavelength shifts of the sharp fringes in the transmission spectra near the stop band edges of a resonant grating-based cavity are monitored. Such fringes are the results of Fabry–Perot resonances of the Bloch modes propagating in the cavity. Two sensor configurations have been considered, a first one for measuring the concentration of a single compound dissolved in water in the vicinity of the grating (bulk sensing), and a second one for determining the concentration of a specific compound adsorbed at the grating surface from a watery mixture of many compounds (surface sensing). In the latter, a thin interface layer which contains receptors specific to the targeted analyte, the PepN enzyme, is applied on top of the grated waveguide section. Filling of the receptors can be effectively seen as growth of an adlayer. Experimentally resolutions of 6×10−6 refractive index unit and ∼4 pm adlayer growth have been obtained for bulk and surface sensing, respectively. With a statistical analysis the limitations to obtain lower resolutions with the current set-up are identified. The compact devices (footprint ∼200 μm × 15 μm) are well suited for multi-sensing in lab-on-a-chip systems and can easily be fabricated with standard micro-fluidic and CMOS technologies.",
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    On-chip bulk-index concentration and direct, label-free protein sensing utilizing an optical grated-waveguide cavity. / Pham Van So, P.V.S.; Dijkstra, Mindert; Hollink, Anton; Kauppinen, L.J.; de Ridder, R.M.; Pollnau, Markus; Lambeck, Paul; Hoekstra, Hugo.

    In: Sensors and actuators. B: Chemical, Vol. 174, 01.11.2012, p. 602-608.

    Research output: Contribution to journalArticleAcademicpeer-review

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    AU - Pham Van So, P.V.S.

    AU - Dijkstra, Mindert

    AU - Hollink, Anton

    AU - Kauppinen, L.J.

    AU - de Ridder, R.M.

    AU - Pollnau, Markus

    AU - Lambeck, Paul

    AU - Hoekstra, Hugo

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    AB - A theoretical and experimental evaluation is given of a principle of direct, label-free opto-chemical sensing. According to this principle, which is applicable in compact planar optical sensors, measurand-induced wavelength shifts of the sharp fringes in the transmission spectra near the stop band edges of a resonant grating-based cavity are monitored. Such fringes are the results of Fabry–Perot resonances of the Bloch modes propagating in the cavity. Two sensor configurations have been considered, a first one for measuring the concentration of a single compound dissolved in water in the vicinity of the grating (bulk sensing), and a second one for determining the concentration of a specific compound adsorbed at the grating surface from a watery mixture of many compounds (surface sensing). In the latter, a thin interface layer which contains receptors specific to the targeted analyte, the PepN enzyme, is applied on top of the grated waveguide section. Filling of the receptors can be effectively seen as growth of an adlayer. Experimentally resolutions of 6×10−6 refractive index unit and ∼4 pm adlayer growth have been obtained for bulk and surface sensing, respectively. With a statistical analysis the limitations to obtain lower resolutions with the current set-up are identified. The compact devices (footprint ∼200 μm × 15 μm) are well suited for multi-sensing in lab-on-a-chip systems and can easily be fabricated with standard micro-fluidic and CMOS technologies.

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    KW - IOMS-SNS: SENSORS

    KW - Grated-waveguide cavity

    KW - Label-free biosensing

    KW - Bulk sensing

    KW - Surface sensing

    KW - METIS-289750

    KW - IR-82113

    KW - Statistical Analysis

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    DO - 10.1016/j.snb.2012.08.002

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    JO - Sensors and actuators. B: Chemical

    JF - Sensors and actuators. B: Chemical

    SN - 0925-4005

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