On-Chip Detection of Beads with a New Electrical Impedance Sensor

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Abstract

Electrical impedance measurements in microfluidic chips are used for single cell analysis. Parallel electrodes are more suited than planar ones since the electrical field distribution is more homogenous. Previous studies showed methods to make parallel electrodes by incorporating an additional layer between two glass wafers, making electrical connections to both sides needed. Also alignment of electrodes is necessary, making the fabrication of parallel electrodes more elaborate. Therefore a new, simpler fabrication method is developed for the fabrication of parallel electrode chips by incorporating a floating electrode in the microchannel just by adding one step in the fabrication process. In this way, only one side of the chip contains electrical connections. Finally, electrical impedance measurements with 3 and 6 µm polystyrene beads were done. All beads were detected and we have shown that it is possible to distinguish the two beads sizes from each other with a confidence level of 95%, based on the relative change in the electrical impedance.
Original languageUndefined
Title of host publicationIEEE Sensors 2010 Conference
Place of PublicationPiscataway
PublisherIEEE
Pages218-221
Number of pages4
ISBN (Print)978-1-4244-8168-2
DOIs
Publication statusPublished - 2 Nov 2010
Event9th IEEE Sensors 2010 - Hilton Waikoloa Village, Waikoloa, United States
Duration: 1 Nov 20104 Nov 2010
Conference number: 9

Publication series

NameThe 9th Annual IEEE Conference on Sensors
PublisherIEEE

Conference

Conference9th IEEE Sensors 2010
CountryUnited States
CityWaikoloa
Period1/11/104/11/10

Keywords

  • IR-75994
  • EWI-19566
  • METIS-275926

Cite this

Segerink, L. I., Sprenkels, A. J., Bomer, J. G., & van den Berg, A. (2010). On-Chip Detection of Beads with a New Electrical Impedance Sensor. In IEEE Sensors 2010 Conference (pp. 218-221). (The 9th Annual IEEE Conference on Sensors). Piscataway: IEEE. https://doi.org/10.1109/ICSENS.2010.5690430
Segerink, Loes Irene ; Sprenkels, A.J. ; Bomer, Johan G. ; van den Berg, Albert. / On-Chip Detection of Beads with a New Electrical Impedance Sensor. IEEE Sensors 2010 Conference. Piscataway : IEEE, 2010. pp. 218-221 (The 9th Annual IEEE Conference on Sensors).
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title = "On-Chip Detection of Beads with a New Electrical Impedance Sensor",
abstract = "Electrical impedance measurements in microfluidic chips are used for single cell analysis. Parallel electrodes are more suited than planar ones since the electrical field distribution is more homogenous. Previous studies showed methods to make parallel electrodes by incorporating an additional layer between two glass wafers, making electrical connections to both sides needed. Also alignment of electrodes is necessary, making the fabrication of parallel electrodes more elaborate. Therefore a new, simpler fabrication method is developed for the fabrication of parallel electrode chips by incorporating a floating electrode in the microchannel just by adding one step in the fabrication process. In this way, only one side of the chip contains electrical connections. Finally, electrical impedance measurements with 3 and 6 µm polystyrene beads were done. All beads were detected and we have shown that it is possible to distinguish the two beads sizes from each other with a confidence level of 95{\%}, based on the relative change in the electrical impedance.",
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author = "Segerink, {Loes Irene} and A.J. Sprenkels and Bomer, {Johan G.} and {van den Berg}, Albert",
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isbn = "978-1-4244-8168-2",
series = "The 9th Annual IEEE Conference on Sensors",
publisher = "IEEE",
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}

Segerink, LI, Sprenkels, AJ, Bomer, JG & van den Berg, A 2010, On-Chip Detection of Beads with a New Electrical Impedance Sensor. in IEEE Sensors 2010 Conference. The 9th Annual IEEE Conference on Sensors, IEEE, Piscataway, pp. 218-221, 9th IEEE Sensors 2010, Waikoloa, United States, 1/11/10. https://doi.org/10.1109/ICSENS.2010.5690430

On-Chip Detection of Beads with a New Electrical Impedance Sensor. / Segerink, Loes Irene; Sprenkels, A.J.; Bomer, Johan G.; van den Berg, Albert.

IEEE Sensors 2010 Conference. Piscataway : IEEE, 2010. p. 218-221 (The 9th Annual IEEE Conference on Sensors).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - On-Chip Detection of Beads with a New Electrical Impedance Sensor

AU - Segerink, Loes Irene

AU - Sprenkels, A.J.

AU - Bomer, Johan G.

AU - van den Berg, Albert

N1 - 10.1109/ICSENS.2010.5690430

PY - 2010/11/2

Y1 - 2010/11/2

N2 - Electrical impedance measurements in microfluidic chips are used for single cell analysis. Parallel electrodes are more suited than planar ones since the electrical field distribution is more homogenous. Previous studies showed methods to make parallel electrodes by incorporating an additional layer between two glass wafers, making electrical connections to both sides needed. Also alignment of electrodes is necessary, making the fabrication of parallel electrodes more elaborate. Therefore a new, simpler fabrication method is developed for the fabrication of parallel electrode chips by incorporating a floating electrode in the microchannel just by adding one step in the fabrication process. In this way, only one side of the chip contains electrical connections. Finally, electrical impedance measurements with 3 and 6 µm polystyrene beads were done. All beads were detected and we have shown that it is possible to distinguish the two beads sizes from each other with a confidence level of 95%, based on the relative change in the electrical impedance.

AB - Electrical impedance measurements in microfluidic chips are used for single cell analysis. Parallel electrodes are more suited than planar ones since the electrical field distribution is more homogenous. Previous studies showed methods to make parallel electrodes by incorporating an additional layer between two glass wafers, making electrical connections to both sides needed. Also alignment of electrodes is necessary, making the fabrication of parallel electrodes more elaborate. Therefore a new, simpler fabrication method is developed for the fabrication of parallel electrode chips by incorporating a floating electrode in the microchannel just by adding one step in the fabrication process. In this way, only one side of the chip contains electrical connections. Finally, electrical impedance measurements with 3 and 6 µm polystyrene beads were done. All beads were detected and we have shown that it is possible to distinguish the two beads sizes from each other with a confidence level of 95%, based on the relative change in the electrical impedance.

KW - IR-75994

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KW - METIS-275926

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Segerink LI, Sprenkels AJ, Bomer JG, van den Berg A. On-Chip Detection of Beads with a New Electrical Impedance Sensor. In IEEE Sensors 2010 Conference. Piscataway: IEEE. 2010. p. 218-221. (The 9th Annual IEEE Conference on Sensors). https://doi.org/10.1109/ICSENS.2010.5690430