On-chip electrokinetic sample focusing for microarray-based biomolecular interaction assays

G. Krishnamoorthy, Edwin Carlen, D. Kohlheyer, Richardus B.M. Schasfoort, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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Abstract

Label-free measurement techniques, such as surface plasmon resonance (SPR) and surface plasmon resonance imaging (iSPR), are emerging as important complementary methods to conventional bioassays because real-time binding kinetics information can be determined[1]. Recent advances in iSPR are becoming increasingly important to label-free microarray-based assay applications, where multiple biomolecular interactions can be measured simultaneously. However, conventional iSPR microarray assay approaches rely on protein printing techniques for immobilization of the ligand to the gold imaging surface[2]. The combination of microfluidics with iSPR allows the in-situ immobilization of ligands and subsequent analyte transport for the biomolecular interaction assay[3]. In this work, we present an integrated microfluidics and iSPR platform, where ligand immobilization and subsequent sample solutions are precisely positioned with an electroosmotically driven address-flow guiding technique.
Original languageEnglish
Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Place of PublicationCalifornia
PublisherChemical and Biological Micro Systems Society
Pages311-313
Number of pages3
Publication statusPublished - 12 Oct 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2008 - San Diego, United States
Duration: 12 Oct 200816 Oct 2008
Conference number: 12

Publication series

NamemicroTAS
PublisherChemical and Biological Micro Systems Society
Volume2
ISSN (Print)1556-5890

Conference

Conference12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2008
Abbreviated titleMicroTAS
CountryUnited States
CitySan Diego
Period12/10/0816/10/08

Keywords

  • IR-68800
  • METIS-264210
  • imaging surfaceplasmon resonance
  • Biomolecular interactions
  • EWI-16950
  • Electrokinetic focusing

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