On-Chip Electrophoresis in Supported Lipid Bilayer Membranes Achieved Using Low Potentials

Jasper van Weerd; Sven Krabbenborg; Jan C.T. Eijkel; Hermanus Bernardus Johannes Karperien; Jurriaan Huskens; Pascal Jonkheijm

doi:10.1021/ja411287u

On-Chip Electrophoresis in Supported Lipid Bilayer Membranes Achieved Using Low Potentials

Jasper van Weerd, Sven Krabbenborg, Jan C.T. Eijkel, Hermanus Bernardus Johannes Karperien, Jurriaan Huskens, Pascal Jonkheijm

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Abstract

A micro supported lipid bilayer (SLB) electrophoresis method was developed, which functions at low potentials and appreciable operating times. To this end, (hydroxymethyl)-ferrocene (FcCH2OH) was employed to provide an electrochemical reaction at the anode and cathode at low applied potential to avoid electrolysis of water. The addition of FcCH2OH did not alter the SLB characteristics or affect biomolecule function, and pH and temperature variations and bubble formation were eliminated. Applying potentials of 0.25–1.2 V during flow gave homogeneous electrical fields and a fast, reversible, and strong build-up of a charged dye-modified lipid in the direction of the oppositely charged electrode. Moreover, streptavidin mobility could be modulated. This method paves the way for further development of analytical devices.

Original language	English
Pages (from-to)	100-103
Journal	Journal of the American Chemical Society
Volume	136
Issue number	1
DOIs	https://doi.org/10.1021/ja411287u
Publication status	Published - 17 Dec 2014

Keywords

IR-88575
METIS-300714

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10.1021/ja411287u

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title = "On-Chip Electrophoresis in Supported Lipid Bilayer Membranes Achieved Using Low Potentials",

abstract = "A micro supported lipid bilayer (SLB) electrophoresis method was developed, which functions at low potentials and appreciable operating times. To this end, (hydroxymethyl)-ferrocene (FcCH2OH) was employed to provide an electrochemical reaction at the anode and cathode at low applied potential to avoid electrolysis of water. The addition of FcCH2OH did not alter the SLB characteristics or affect biomolecule function, and pH and temperature variations and bubble formation were eliminated. Applying potentials of 0.25–1.2 V during flow gave homogeneous electrical fields and a fast, reversible, and strong build-up of a charged dye-modified lipid in the direction of the oppositely charged electrode. Moreover, streptavidin mobility could be modulated. This method paves the way for further development of analytical devices.",

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T1 - On-Chip Electrophoresis in Supported Lipid Bilayer Membranes Achieved Using Low Potentials

AU - van Weerd, Jasper

AU - Krabbenborg, Sven

AU - Eijkel, Jan C.T.

AU - Karperien, Hermanus Bernardus Johannes

AU - Huskens, Jurriaan

AU - Jonkheijm, Pascal

PY - 2014/12/17

Y1 - 2014/12/17

N2 - A micro supported lipid bilayer (SLB) electrophoresis method was developed, which functions at low potentials and appreciable operating times. To this end, (hydroxymethyl)-ferrocene (FcCH2OH) was employed to provide an electrochemical reaction at the anode and cathode at low applied potential to avoid electrolysis of water. The addition of FcCH2OH did not alter the SLB characteristics or affect biomolecule function, and pH and temperature variations and bubble formation were eliminated. Applying potentials of 0.25–1.2 V during flow gave homogeneous electrical fields and a fast, reversible, and strong build-up of a charged dye-modified lipid in the direction of the oppositely charged electrode. Moreover, streptavidin mobility could be modulated. This method paves the way for further development of analytical devices.

AB - A micro supported lipid bilayer (SLB) electrophoresis method was developed, which functions at low potentials and appreciable operating times. To this end, (hydroxymethyl)-ferrocene (FcCH2OH) was employed to provide an electrochemical reaction at the anode and cathode at low applied potential to avoid electrolysis of water. The addition of FcCH2OH did not alter the SLB characteristics or affect biomolecule function, and pH and temperature variations and bubble formation were eliminated. Applying potentials of 0.25–1.2 V during flow gave homogeneous electrical fields and a fast, reversible, and strong build-up of a charged dye-modified lipid in the direction of the oppositely charged electrode. Moreover, streptavidin mobility could be modulated. This method paves the way for further development of analytical devices.

KW - IR-88575

KW - METIS-300714

U2 - 10.1021/ja411287u

DO - 10.1021/ja411287u

M3 - Article

SN - 0002-7863

VL - 136

SP - 100

EP - 103

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 1

ER -

On-Chip Electrophoresis in Supported Lipid Bilayer Membranes Achieved Using Low Potentials

Abstract

Keywords

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