On-Chip Electrophoresis in Supported Lipid Bilayer Membranes Achieved Using Low Potentials

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9 Citations (Scopus)

Abstract

A micro supported lipid bilayer (SLB) electrophoresis method was developed, which functions at low potentials and appreciable operating times. To this end, (hydroxymethyl)-ferrocene (FcCH2OH) was employed to provide an electrochemical reaction at the anode and cathode at low applied potential to avoid electrolysis of water. The addition of FcCH2OH did not alter the SLB characteristics or affect biomolecule function, and pH and temperature variations and bubble formation were eliminated. Applying potentials of 0.25–1.2 V during flow gave homogeneous electrical fields and a fast, reversible, and strong build-up of a charged dye-modified lipid in the direction of the oppositely charged electrode. Moreover, streptavidin mobility could be modulated. This method paves the way for further development of analytical devices.
Original languageEnglish
Pages (from-to)100-103
JournalJournal of the American Chemical Society
Volume136
Issue number1
DOIs
Publication statusPublished - 17 Dec 2014

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Lipid bilayers
Lipid Bilayers
Membrane Lipids
Electrophoresis
Electrodes
Membranes
Bubble formation
Streptavidin
Biomolecules
Electrolysis
Lipids
Anodes
Cathodes
Coloring Agents
Dyes
Water
Equipment and Supplies
Temperature
Direction compound
ferrocene

Keywords

  • IR-88575
  • METIS-300714

Cite this

@article{4133094e0a584f7a89f552ee4640da26,
title = "On-Chip Electrophoresis in Supported Lipid Bilayer Membranes Achieved Using Low Potentials",
abstract = "A micro supported lipid bilayer (SLB) electrophoresis method was developed, which functions at low potentials and appreciable operating times. To this end, (hydroxymethyl)-ferrocene (FcCH2OH) was employed to provide an electrochemical reaction at the anode and cathode at low applied potential to avoid electrolysis of water. The addition of FcCH2OH did not alter the SLB characteristics or affect biomolecule function, and pH and temperature variations and bubble formation were eliminated. Applying potentials of 0.25–1.2 V during flow gave homogeneous electrical fields and a fast, reversible, and strong build-up of a charged dye-modified lipid in the direction of the oppositely charged electrode. Moreover, streptavidin mobility could be modulated. This method paves the way for further development of analytical devices.",
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author = "{van Weerd}, Jasper and Sven Krabbenborg and Eijkel, {Jan C.T.} and Karperien, {Hermanus Bernardus Johannes} and Jurriaan Huskens and Pascal Jonkheijm",
year = "2014",
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day = "17",
doi = "10.1021/ja411287u",
language = "English",
volume = "136",
pages = "100--103",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
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On-Chip Electrophoresis in Supported Lipid Bilayer Membranes Achieved Using Low Potentials. / van Weerd, Jasper; Krabbenborg, Sven; Eijkel, Jan C.T.; Karperien, Hermanus Bernardus Johannes; Huskens, Jurriaan; Jonkheijm, Pascal.

In: Journal of the American Chemical Society, Vol. 136, No. 1, 17.12.2014, p. 100-103.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - On-Chip Electrophoresis in Supported Lipid Bilayer Membranes Achieved Using Low Potentials

AU - van Weerd, Jasper

AU - Krabbenborg, Sven

AU - Eijkel, Jan C.T.

AU - Karperien, Hermanus Bernardus Johannes

AU - Huskens, Jurriaan

AU - Jonkheijm, Pascal

PY - 2014/12/17

Y1 - 2014/12/17

N2 - A micro supported lipid bilayer (SLB) electrophoresis method was developed, which functions at low potentials and appreciable operating times. To this end, (hydroxymethyl)-ferrocene (FcCH2OH) was employed to provide an electrochemical reaction at the anode and cathode at low applied potential to avoid electrolysis of water. The addition of FcCH2OH did not alter the SLB characteristics or affect biomolecule function, and pH and temperature variations and bubble formation were eliminated. Applying potentials of 0.25–1.2 V during flow gave homogeneous electrical fields and a fast, reversible, and strong build-up of a charged dye-modified lipid in the direction of the oppositely charged electrode. Moreover, streptavidin mobility could be modulated. This method paves the way for further development of analytical devices.

AB - A micro supported lipid bilayer (SLB) electrophoresis method was developed, which functions at low potentials and appreciable operating times. To this end, (hydroxymethyl)-ferrocene (FcCH2OH) was employed to provide an electrochemical reaction at the anode and cathode at low applied potential to avoid electrolysis of water. The addition of FcCH2OH did not alter the SLB characteristics or affect biomolecule function, and pH and temperature variations and bubble formation were eliminated. Applying potentials of 0.25–1.2 V during flow gave homogeneous electrical fields and a fast, reversible, and strong build-up of a charged dye-modified lipid in the direction of the oppositely charged electrode. Moreover, streptavidin mobility could be modulated. This method paves the way for further development of analytical devices.

KW - IR-88575

KW - METIS-300714

U2 - 10.1021/ja411287u

DO - 10.1021/ja411287u

M3 - Article

VL - 136

SP - 100

EP - 103

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

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