On-chip real-time monitoring of multiple displacement amplification of DNA

B. B. Bruijns*, F. Costantini, N. Lovecchio, R. M. Tiggelaar, G. Di Timoteo, A. Nascetti, G. de Cesare, J. G. E. Gardeniers, D. Caputo

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

The integration of systems for real-time monitoring of DNA amplification reactions is rather limited, because the heating system, the temperature control and the detection system are usually realized with separate modules. In this work, a lab-on-a-chip system for real-time monitoring of the multiple displacement amplification reaction (MDA) is presented. The amplification and detection unit consists of a system-on-glass (SoG) coupled to a microfluidic chip. The SoG includes thin film metallic resistors and amorphous silicon temperature sensors to control the temperature, as well as amorphous silicon photosensors and an interference filter for fluorescence detection on a single glass plate. The amplification reaction is carried out in the microfluidic chip made of cyclic olefin copolymer (COC). By using this setup, the multiple displacement amplification reaction can be monitored in real-time using the fluorophore [Ru(phen)2(dppz)]2+.
Original languageEnglish
Pages (from-to)16-22
Number of pages7
JournalSensors and actuators. B: Chemical
Volume293
DOIs
Publication statusPublished - 15 Aug 2019

Keywords

  • MDA
  • DNA amplification
  • Real-time monitoring
  • Fluorescence
  • Lab-on-a-chip

Cite this