On-chip sample preparation by controlled release of antibodies for simple CD4 counting

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26 Citations (Scopus)

Abstract

We present a simple system for CD4 and CD8 counting for point-of-care HIV staging in low-resource settings. Automatic sample preparation is achieved through a dried reagent coating inside a thin (26 μm) counting chamber, allowing the delayed release of fluorochrome conjugated monoclonal antibodies after the filling of the chamber with whole blood by capillary flow. A custom-built image cytometer is used to capture fluorescence images representing more than 1 μl of blood. The thin layer of blood in combination with the large image area allows the use of whole blood from a finger prick without the need for dilution, lysis or cell enrichment. Automatic cell counting of CD4+ and CD8+ T-lymphocytes correlates well with results obtained by flow cytometry
Original languageEnglish
Pages (from-to)167-173
JournalLab on a chip
Volume12
DOIs
Publication statusPublished - 2012

Keywords

  • IR-84644
  • METIS-281892

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