On-chip sample preparation by controlled release of antibodies for simple CD4 counting

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Scopus)

Abstract

We present a simple system for CD4 and CD8 counting for point-of-care HIV staging in low-resource settings. Automatic sample preparation is achieved through a dried reagent coating inside a thin (26 μm) counting chamber, allowing the delayed release of fluorochrome conjugated monoclonal antibodies after the filling of the chamber with whole blood by capillary flow. A custom-built image cytometer is used to capture fluorescence images representing more than 1 μl of blood. The thin layer of blood in combination with the large image area allows the use of whole blood from a finger prick without the need for dilution, lysis or cell enrichment. Automatic cell counting of CD4+ and CD8+ T-lymphocytes correlates well with results obtained by flow cytometry
Original languageEnglish
Pages (from-to)167-173
JournalLab on a chip
Volume12
DOIs
Publication statusPublished - 2012

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Delayed-Action Preparations
Antibodies
Blood
Point-of-Care Systems
Capillary flow
Monoclonal antibodies
T-cells
Flow cytometry
Fluorescent Dyes
Dilution
Fingers
Flow Cytometry
Fluorescence
Monoclonal Antibodies
HIV
T-Lymphocytes
Coatings

Keywords

  • IR-84644
  • METIS-281892

Cite this

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title = "On-chip sample preparation by controlled release of antibodies for simple CD4 counting",
abstract = "We present a simple system for CD4 and CD8 counting for point-of-care HIV staging in low-resource settings. Automatic sample preparation is achieved through a dried reagent coating inside a thin (26 μm) counting chamber, allowing the delayed release of fluorochrome conjugated monoclonal antibodies after the filling of the chamber with whole blood by capillary flow. A custom-built image cytometer is used to capture fluorescence images representing more than 1 μl of blood. The thin layer of blood in combination with the large image area allows the use of whole blood from a finger prick without the need for dilution, lysis or cell enrichment. Automatic cell counting of CD4+ and CD8+ T-lymphocytes correlates well with results obtained by flow cytometry",
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year = "2012",
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On-chip sample preparation by controlled release of antibodies for simple CD4 counting. / Beck, Markus; Brockhuis, S.; van der Velde, N.; Breukers, Christian; Greve, Jan; Terstappen, Leonardus Wendelinus Mathias Marie.

In: Lab on a chip, Vol. 12, 2012, p. 167-173.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - On-chip sample preparation by controlled release of antibodies for simple CD4 counting

AU - Beck, Markus

AU - Brockhuis, S.

AU - van der Velde, N.

AU - Breukers, Christian

AU - Greve, Jan

AU - Terstappen, Leonardus Wendelinus Mathias Marie

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AB - We present a simple system for CD4 and CD8 counting for point-of-care HIV staging in low-resource settings. Automatic sample preparation is achieved through a dried reagent coating inside a thin (26 μm) counting chamber, allowing the delayed release of fluorochrome conjugated monoclonal antibodies after the filling of the chamber with whole blood by capillary flow. A custom-built image cytometer is used to capture fluorescence images representing more than 1 μl of blood. The thin layer of blood in combination with the large image area allows the use of whole blood from a finger prick without the need for dilution, lysis or cell enrichment. Automatic cell counting of CD4+ and CD8+ T-lymphocytes correlates well with results obtained by flow cytometry

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KW - METIS-281892

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