On-chip sample preparation by controlled release of antibodies for simple CD4 counting

Markus Beck, S. Brockhuis, N. van der Velde, Christian Breukers, Jan Greve, Leonardus Wendelinus Mathias Marie Terstappen

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

We present a simple system for CD4 and CD8 counting for point-of-care HIV staging in low-resource settings. Automatic sample preparation is achieved through a dried reagent coating inside a thin (26 μm) counting chamber, allowing the delayed release of fluorochrome conjugated monoclonal antibodies after the filling of the chamber with whole blood by capillary flow. A custom-built image cytometer is used to capture fluorescence images representing more than 1 μl of blood. The thin layer of blood in combination with the large image area allows the use of whole blood from a finger prick without the need for dilution, lysis or cell enrichment. Automatic cell counting of CD4+ and CD8+ T-lymphocytes correlates well with results obtained by flow cytometry
Original languageEnglish
Pages (from-to)167-173
JournalLab on a chip
Volume12
DOIs
Publication statusPublished - 2012

Keywords

  • IR-84644
  • METIS-281892

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