On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman

A.G. Shvedko; S.G. Kruglik; V.V. Ermolenkov; V.A. Orlovich; P.Y. Turpin; J. Greve; C. Otto

doi:10.1007/978-94-011-4479-7_116

On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman

A.G. Shvedko, S.G. Kruglik, V.V. Ermolenkov, V.A. Orlovich, P.Y. Turpin, J. Greve, C. Otto

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

Interaction of water-soluble cationic copper(II) 5, 10, 15, 20-tetrakis[4-(N-methylpyridyl)] porphyrin (Cu(TMpy-P4)) with DNA attracts considerable attention and has been studied by a variety of techniques [1–3]. Cu(TMpy-P4) is known as a good intercalator into B-form DNA; the results of numerous studies [1–3] are consistent with copper porphyrin intercalation between consecutive GC base pairs or next to a GC base pair. However, porphyrin groove-binding at AT-rich regions of DNA also remains a possibility [3]. In cw Raman spectra, Cu(TMpy-P4) mixed with nucleic acids exhibit porphyrin core vibrations near 1570 (V2) and 1368 (V4) cm-1 , but under pulsed, high power excitation extra bands appear at 1550 (v2*) and 1346 (V4*) cm-1, which originate from a transient exciplex which can be formed at AT sites of the double helix [4–6]. These facts were discussed in [5] and, since Cu(TMpy-P4) is usually considered as preferentially intercalated, the mechanism of fast porphyrin translocation from GC to AT site has been proposed on the basis of nanosecond RR data [5].

Original language	English
Title of host publication	Spectroscopy of Biological Molecules: New Directions
Subtitle of host publication	8th European Conference on the Spectroscopy of Biological Molecules, 29 August-2 September 1999, Enschede, The Netherlands
Editors	Jan Greve, Gerwin J. Puppels, Cees Otto
Place of Publication	Dordrecht
Publisher	Kluwer Academic
Pages	265-266
Number of pages	2
ISBN (Electronic)	978-94-011-4479-7
ISBN (Print)	978-94-010-5919-0
DOIs	https://doi.org/10.1007/978-94-011-4479-7_116
Publication status	Published - 15 Oct 1999

Keywords

Double Helix
Stimulate Raman Scattering
Marie Curie
Stokes component
Stepanov Institute

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10.1007/978-94-011-4479-7_116

Cite this

Shvedko, A. G., Kruglik, S. G., Ermolenkov, V. V., Orlovich, V. A., Turpin, P. Y., Greve, J., & Otto, C. (1999). On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman. In J. Greve, G. J. Puppels, & C. Otto (Eds.), Spectroscopy of Biological Molecules: New Directions: 8th European Conference on the Spectroscopy of Biological Molecules, 29 August-2 September 1999, Enschede, The Netherlands (pp. 265-266). Kluwer Academic. https://doi.org/10.1007/978-94-011-4479-7_116

Shvedko, A.G. ; Kruglik, S.G. ; Ermolenkov, V.V. et al. / On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman. Spectroscopy of Biological Molecules: New Directions: 8th European Conference on the Spectroscopy of Biological Molecules, 29 August-2 September 1999, Enschede, The Netherlands. editor / Jan Greve ; Gerwin J. Puppels ; Cees Otto. Dordrecht : Kluwer Academic, 1999. pp. 265-266

@inproceedings{649474485e9b4e94aad31bf14443dd4a,

title = "On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman",

abstract = "Interaction of water-soluble cationic copper(II) 5, 10, 15, 20-tetrakis[4-(N-methylpyridyl)] porphyrin (Cu(TMpy-P4)) with DNA attracts considerable attention and has been studied by a variety of techniques [1–3]. Cu(TMpy-P4) is known as a good intercalator into B-form DNA; the results of numerous studies [1–3] are consistent with copper porphyrin intercalation between consecutive GC base pairs or next to a GC base pair. However, porphyrin groove-binding at AT-rich regions of DNA also remains a possibility [3]. In cw Raman spectra, Cu(TMpy-P4) mixed with nucleic acids exhibit porphyrin core vibrations near 1570 (V2) and 1368 (V4) cm-1 , but under pulsed, high power excitation extra bands appear at 1550 (v2*) and 1346 (V4*) cm-1, which originate from a transient exciplex which can be formed at AT sites of the double helix [4–6]. These facts were discussed in [5] and, since Cu(TMpy-P4) is usually considered as preferentially intercalated, the mechanism of fast porphyrin translocation from GC to AT site has been proposed on the basis of nanosecond RR data [5].",

keywords = "Double Helix, Stimulate Raman Scattering, Marie Curie, Stokes component, Stepanov Institute",

author = "A.G. Shvedko and S.G. Kruglik and V.V. Ermolenkov and V.A. Orlovich and P.Y. Turpin and J. Greve and C. Otto",

year = "1999",

month = oct,

day = "15",

doi = "10.1007/978-94-011-4479-7_116",

language = "English",

isbn = "978-94-010-5919-0",

pages = "265--266",

editor = "Jan Greve and Puppels, {Gerwin J.} and Cees Otto",

booktitle = "Spectroscopy of Biological Molecules: New Directions",

publisher = "Kluwer Academic",

}

Shvedko, AG, Kruglik, SG, Ermolenkov, VV, Orlovich, VA, Turpin, PY, Greve, J & Otto, C 1999, On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman. in J Greve, GJ Puppels & C Otto (eds), Spectroscopy of Biological Molecules: New Directions: 8th European Conference on the Spectroscopy of Biological Molecules, 29 August-2 September 1999, Enschede, The Netherlands. Kluwer Academic, Dordrecht, pp. 265-266. https://doi.org/10.1007/978-94-011-4479-7_116

On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman. / Shvedko, A.G.; Kruglik, S.G.; Ermolenkov, V.V. et al.
Spectroscopy of Biological Molecules: New Directions: 8th European Conference on the Spectroscopy of Biological Molecules, 29 August-2 September 1999, Enschede, The Netherlands. ed. / Jan Greve; Gerwin J. Puppels; Cees Otto. Dordrecht: Kluwer Academic, 1999. p. 265-266.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman

AU - Shvedko, A.G.

AU - Kruglik, S.G.

AU - Ermolenkov, V.V.

AU - Orlovich, V.A.

AU - Turpin, P.Y.

AU - Greve, J.

AU - Otto, C.

PY - 1999/10/15

Y1 - 1999/10/15

N2 - Interaction of water-soluble cationic copper(II) 5, 10, 15, 20-tetrakis[4-(N-methylpyridyl)] porphyrin (Cu(TMpy-P4)) with DNA attracts considerable attention and has been studied by a variety of techniques [1–3]. Cu(TMpy-P4) is known as a good intercalator into B-form DNA; the results of numerous studies [1–3] are consistent with copper porphyrin intercalation between consecutive GC base pairs or next to a GC base pair. However, porphyrin groove-binding at AT-rich regions of DNA also remains a possibility [3]. In cw Raman spectra, Cu(TMpy-P4) mixed with nucleic acids exhibit porphyrin core vibrations near 1570 (V2) and 1368 (V4) cm-1 , but under pulsed, high power excitation extra bands appear at 1550 (v2*) and 1346 (V4*) cm-1, which originate from a transient exciplex which can be formed at AT sites of the double helix [4–6]. These facts were discussed in [5] and, since Cu(TMpy-P4) is usually considered as preferentially intercalated, the mechanism of fast porphyrin translocation from GC to AT site has been proposed on the basis of nanosecond RR data [5].

AB - Interaction of water-soluble cationic copper(II) 5, 10, 15, 20-tetrakis[4-(N-methylpyridyl)] porphyrin (Cu(TMpy-P4)) with DNA attracts considerable attention and has been studied by a variety of techniques [1–3]. Cu(TMpy-P4) is known as a good intercalator into B-form DNA; the results of numerous studies [1–3] are consistent with copper porphyrin intercalation between consecutive GC base pairs or next to a GC base pair. However, porphyrin groove-binding at AT-rich regions of DNA also remains a possibility [3]. In cw Raman spectra, Cu(TMpy-P4) mixed with nucleic acids exhibit porphyrin core vibrations near 1570 (V2) and 1368 (V4) cm-1 , but under pulsed, high power excitation extra bands appear at 1550 (v2*) and 1346 (V4*) cm-1, which originate from a transient exciplex which can be formed at AT sites of the double helix [4–6]. These facts were discussed in [5] and, since Cu(TMpy-P4) is usually considered as preferentially intercalated, the mechanism of fast porphyrin translocation from GC to AT site has been proposed on the basis of nanosecond RR data [5].

KW - Double Helix

KW - Stimulate Raman Scattering

KW - Marie Curie

KW - Stokes component

KW - Stepanov Institute

U2 - 10.1007/978-94-011-4479-7_116

DO - 10.1007/978-94-011-4479-7_116

M3 - Conference contribution

SN - 978-94-010-5919-0

SP - 265

EP - 266

BT - Spectroscopy of Biological Molecules: New Directions

A2 - Greve, Jan

A2 - Puppels, Gerwin J.

A2 - Otto, Cees

PB - Kluwer Academic

CY - Dordrecht

ER -

Shvedko AG, Kruglik SG, Ermolenkov VV, Orlovich VA, Turpin PY, Greve J et al. On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman. In Greve J, Puppels GJ, Otto C, editors, Spectroscopy of Biological Molecules: New Directions: 8th European Conference on the Spectroscopy of Biological Molecules, 29 August-2 September 1999, Enschede, The Netherlands. Dordrecht: Kluwer Academic. 1999. p. 265-266 doi: 10.1007/978-94-011-4479-7_116

On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman

Abstract

Keywords

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