On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman

A.G. Shvedko, S.G. Kruglik, V.V. Ermolenkov, V.A. Orlovich, P.Y. Turpin, J. Greve, C. Otto

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    Abstract

    Interaction of water-soluble cationic copper(II) 5, 10, 15, 20-tetrakis[4-(N-methylpyridyl)] porphyrin (Cu(TMpy-P4)) with DNA attracts considerable attention and has been studied by a variety of techniques [1–3]. Cu(TMpy-P4) is known as a good intercalator into B-form DNA; the results of numerous studies [1–3] are consistent with copper porphyrin intercalation between consecutive GC base pairs or next to a GC base pair. However, porphyrin groove-binding at AT-rich regions of DNA also remains a possibility [3]. In cw Raman spectra, Cu(TMpy-P4) mixed with nucleic acids exhibit porphyrin core vibrations near 1570 (V2) and 1368 (V4) cm-1 , but under pulsed, high power excitation extra bands appear at 1550 (v2*) and 1346 (V4*) cm-1, which originate from a transient exciplex which can be formed at AT sites of the double helix [4–6]. These facts were discussed in [5] and, since Cu(TMpy-P4) is usually considered as preferentially intercalated, the mechanism of fast porphyrin translocation from GC to AT site has been proposed on the basis of nanosecond RR data [5].
    Original languageEnglish
    Title of host publicationSpectroscopy of Biological Molecules: New Directions
    Subtitle of host publication8th European Conference on the Spectroscopy of Biological Molecules, 29 August-2 September 1999, Enschede, The Netherlands
    EditorsJan Greve, Gerwin J. Puppels, Cees Otto
    Place of PublicationDordrecht
    PublisherKluwer Academic
    Pages265-266
    Number of pages2
    ISBN (Electronic)978-94-011-4479-7
    ISBN (Print)978-94-010-5919-0
    DOIs
    Publication statusPublished - 15 Oct 1999

    Fingerprint

    Porphyrins
    Copper
    AT Rich Sequence
    B-Form DNA
    Intercalating Agents
    DNA
    Intercalation
    Nucleic Acids
    Raman scattering
    calf thymus DNA
    Water

    Keywords

    • Double Helix
    • Stimulate Raman Scattering
    • Marie Curie
    • Stokes component
    • Stepanov Institute

    Cite this

    Shvedko, A. G., Kruglik, S. G., Ermolenkov, V. V., Orlovich, V. A., Turpin, P. Y., Greve, J., & Otto, C. (1999). On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman. In J. Greve, G. J. Puppels, & C. Otto (Eds.), Spectroscopy of Biological Molecules: New Directions: 8th European Conference on the Spectroscopy of Biological Molecules, 29 August-2 September 1999, Enschede, The Netherlands (pp. 265-266). Dordrecht: Kluwer Academic. https://doi.org/10.1007/978-94-011-4479-7_116
    Shvedko, A.G. ; Kruglik, S.G. ; Ermolenkov, V.V. ; Orlovich, V.A. ; Turpin, P.Y. ; Greve, J. ; Otto, C. / On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman. Spectroscopy of Biological Molecules: New Directions: 8th European Conference on the Spectroscopy of Biological Molecules, 29 August-2 September 1999, Enschede, The Netherlands. editor / Jan Greve ; Gerwin J. Puppels ; Cees Otto. Dordrecht : Kluwer Academic, 1999. pp. 265-266
    @inproceedings{649474485e9b4e94aad31bf14443dd4a,
    title = "On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman",
    abstract = "Interaction of water-soluble cationic copper(II) 5, 10, 15, 20-tetrakis[4-(N-methylpyridyl)] porphyrin (Cu(TMpy-P4)) with DNA attracts considerable attention and has been studied by a variety of techniques [1–3]. Cu(TMpy-P4) is known as a good intercalator into B-form DNA; the results of numerous studies [1–3] are consistent with copper porphyrin intercalation between consecutive GC base pairs or next to a GC base pair. However, porphyrin groove-binding at AT-rich regions of DNA also remains a possibility [3]. In cw Raman spectra, Cu(TMpy-P4) mixed with nucleic acids exhibit porphyrin core vibrations near 1570 (V2) and 1368 (V4) cm-1 , but under pulsed, high power excitation extra bands appear at 1550 (v2*) and 1346 (V4*) cm-1, which originate from a transient exciplex which can be formed at AT sites of the double helix [4–6]. These facts were discussed in [5] and, since Cu(TMpy-P4) is usually considered as preferentially intercalated, the mechanism of fast porphyrin translocation from GC to AT site has been proposed on the basis of nanosecond RR data [5].",
    keywords = "Double Helix, Stimulate Raman Scattering, Marie Curie, Stokes component, Stepanov Institute",
    author = "A.G. Shvedko and S.G. Kruglik and V.V. Ermolenkov and V.A. Orlovich and P.Y. Turpin and J. Greve and C. Otto",
    year = "1999",
    month = "10",
    day = "15",
    doi = "10.1007/978-94-011-4479-7_116",
    language = "English",
    isbn = "978-94-010-5919-0",
    pages = "265--266",
    editor = "Jan Greve and Puppels, {Gerwin J.} and Cees Otto",
    booktitle = "Spectroscopy of Biological Molecules: New Directions",
    publisher = "Kluwer Academic",

    }

    Shvedko, AG, Kruglik, SG, Ermolenkov, VV, Orlovich, VA, Turpin, PY, Greve, J & Otto, C 1999, On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman. in J Greve, GJ Puppels & C Otto (eds), Spectroscopy of Biological Molecules: New Directions: 8th European Conference on the Spectroscopy of Biological Molecules, 29 August-2 September 1999, Enschede, The Netherlands. Kluwer Academic, Dordrecht, pp. 265-266. https://doi.org/10.1007/978-94-011-4479-7_116

    On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman. / Shvedko, A.G.; Kruglik, S.G.; Ermolenkov, V.V.; Orlovich, V.A.; Turpin, P.Y.; Greve, J.; Otto, C.

    Spectroscopy of Biological Molecules: New Directions: 8th European Conference on the Spectroscopy of Biological Molecules, 29 August-2 September 1999, Enschede, The Netherlands. ed. / Jan Greve; Gerwin J. Puppels; Cees Otto. Dordrecht : Kluwer Academic, 1999. p. 265-266.

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    TY - GEN

    T1 - On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman

    AU - Shvedko, A.G.

    AU - Kruglik, S.G.

    AU - Ermolenkov, V.V.

    AU - Orlovich, V.A.

    AU - Turpin, P.Y.

    AU - Greve, J.

    AU - Otto, C.

    PY - 1999/10/15

    Y1 - 1999/10/15

    N2 - Interaction of water-soluble cationic copper(II) 5, 10, 15, 20-tetrakis[4-(N-methylpyridyl)] porphyrin (Cu(TMpy-P4)) with DNA attracts considerable attention and has been studied by a variety of techniques [1–3]. Cu(TMpy-P4) is known as a good intercalator into B-form DNA; the results of numerous studies [1–3] are consistent with copper porphyrin intercalation between consecutive GC base pairs or next to a GC base pair. However, porphyrin groove-binding at AT-rich regions of DNA also remains a possibility [3]. In cw Raman spectra, Cu(TMpy-P4) mixed with nucleic acids exhibit porphyrin core vibrations near 1570 (V2) and 1368 (V4) cm-1 , but under pulsed, high power excitation extra bands appear at 1550 (v2*) and 1346 (V4*) cm-1, which originate from a transient exciplex which can be formed at AT sites of the double helix [4–6]. These facts were discussed in [5] and, since Cu(TMpy-P4) is usually considered as preferentially intercalated, the mechanism of fast porphyrin translocation from GC to AT site has been proposed on the basis of nanosecond RR data [5].

    AB - Interaction of water-soluble cationic copper(II) 5, 10, 15, 20-tetrakis[4-(N-methylpyridyl)] porphyrin (Cu(TMpy-P4)) with DNA attracts considerable attention and has been studied by a variety of techniques [1–3]. Cu(TMpy-P4) is known as a good intercalator into B-form DNA; the results of numerous studies [1–3] are consistent with copper porphyrin intercalation between consecutive GC base pairs or next to a GC base pair. However, porphyrin groove-binding at AT-rich regions of DNA also remains a possibility [3]. In cw Raman spectra, Cu(TMpy-P4) mixed with nucleic acids exhibit porphyrin core vibrations near 1570 (V2) and 1368 (V4) cm-1 , but under pulsed, high power excitation extra bands appear at 1550 (v2*) and 1346 (V4*) cm-1, which originate from a transient exciplex which can be formed at AT sites of the double helix [4–6]. These facts were discussed in [5] and, since Cu(TMpy-P4) is usually considered as preferentially intercalated, the mechanism of fast porphyrin translocation from GC to AT site has been proposed on the basis of nanosecond RR data [5].

    KW - Double Helix

    KW - Stimulate Raman Scattering

    KW - Marie Curie

    KW - Stokes component

    KW - Stepanov Institute

    U2 - 10.1007/978-94-011-4479-7_116

    DO - 10.1007/978-94-011-4479-7_116

    M3 - Conference contribution

    SN - 978-94-010-5919-0

    SP - 265

    EP - 266

    BT - Spectroscopy of Biological Molecules: New Directions

    A2 - Greve, Jan

    A2 - Puppels, Gerwin J.

    A2 - Otto, Cees

    PB - Kluwer Academic

    CY - Dordrecht

    ER -

    Shvedko AG, Kruglik SG, Ermolenkov VV, Orlovich VA, Turpin PY, Greve J et al. On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman. In Greve J, Puppels GJ, Otto C, editors, Spectroscopy of Biological Molecules: New Directions: 8th European Conference on the Spectroscopy of Biological Molecules, 29 August-2 September 1999, Enschede, The Netherlands. Dordrecht: Kluwer Academic. 1999. p. 265-266 https://doi.org/10.1007/978-94-011-4479-7_116