On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman

A.G. Shvedko, S.G. Kruglik, V.V. Ermolenkov, V.A. Orlovich, P.Y. Turpin, J. Greve, C. Otto

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    Abstract

    Interaction of water-soluble cationic copper(II) 5, 10, 15, 20-tetrakis[4-(N-methylpyridyl)] porphyrin (Cu(TMpy-P4)) with DNA attracts considerable attention and has been studied by a variety of techniques [1–3]. Cu(TMpy-P4) is known as a good intercalator into B-form DNA; the results of numerous studies [1–3] are consistent with copper porphyrin intercalation between consecutive GC base pairs or next to a GC base pair. However, porphyrin groove-binding at AT-rich regions of DNA also remains a possibility [3]. In cw Raman spectra, Cu(TMpy-P4) mixed with nucleic acids exhibit porphyrin core vibrations near 1570 (V2) and 1368 (V4) cm-1 , but under pulsed, high power excitation extra bands appear at 1550 (v2*) and 1346 (V4*) cm-1, which originate from a transient exciplex which can be formed at AT sites of the double helix [4–6]. These facts were discussed in [5] and, since Cu(TMpy-P4) is usually considered as preferentially intercalated, the mechanism of fast porphyrin translocation from GC to AT site has been proposed on the basis of nanosecond RR data [5].
    Original languageEnglish
    Title of host publicationSpectroscopy of Biological Molecules: New Directions
    Subtitle of host publication8th European Conference on the Spectroscopy of Biological Molecules, 29 August-2 September 1999, Enschede, The Netherlands
    EditorsJan Greve, Gerwin J. Puppels, Cees Otto
    Place of PublicationDordrecht
    PublisherKluwer Academic
    Pages265-266
    Number of pages2
    ISBN (Electronic)978-94-011-4479-7
    ISBN (Print)978-94-010-5919-0
    DOIs
    Publication statusPublished - 15 Oct 1999

    Keywords

    • Double Helix
    • Stimulate Raman Scattering
    • Marie Curie
    • Stokes component
    • Stepanov Institute

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  • Cite this

    Shvedko, A. G., Kruglik, S. G., Ermolenkov, V. V., Orlovich, V. A., Turpin, P. Y., Greve, J., & Otto, C. (1999). On the mechanism of exciplex formation between Cu(TMpy-P4) and calf-thymus DNA as studied by saturation resonance Raman. In J. Greve, G. J. Puppels, & C. Otto (Eds.), Spectroscopy of Biological Molecules: New Directions: 8th European Conference on the Spectroscopy of Biological Molecules, 29 August-2 September 1999, Enschede, The Netherlands (pp. 265-266). Dordrecht: Kluwer Academic. https://doi.org/10.1007/978-94-011-4479-7_116