TY - JOUR
T1 - On the way back from 3D to 2D
T2 - Chitosan promotes adhesion and development of neuronal networks onto culture supports
AU - Di Lisa, Donatella
AU - Muzzi, Lorenzo
AU - Pepe, Sara
AU - Dellacasa, Elena
AU - Frega, Monica
AU - Fassio, Anna
AU - Martinoia, Sergio
AU - Pastorino, Laura
N1 - Funding Information:
The authors thanks CNPH lab (University of Twente) for providing h-iPSCs rtTa/NgN2 positive line, Marloes R. Levers from CNPH group for teaching stem cell maintaining and differentiation, Chitolytic for kindly providing CHI2 and Maria Teresa Tedesco for support in rat neurons experiments.
Publisher Copyright:
© 2022
PY - 2022/12/1
Y1 - 2022/12/1
N2 - Most in vitro functional and morphological studies for developing nervous system have been performed using traditional monolayer cultures onto supports modified by extracellular matrix components or synthetic biopolymers. These biomolecules act as adhesion factors essential for neuronal growth and differentiation. In this study, the use of chitosan as adhesion factor was investigated. Primary rat neurons and neurons differentiated from human induced pluripotent stem cells were cultured onto chitosan and standard adhesion factors modified supports. The initiation, elongation and branching of neuritic processes, synaptogenesis and electrophysiological behavior were studied. The biopolymers affected neurites outgrowth in a time dependent manner; in particular, chitosan promoted neuronal polarity in both cell cultures. These results indicate chitosan as a valid adhesion factor alternative to the standard ones, with the advantage that it can be used both in 2D and 3D cultures, acting as a bridge between these in vitro models.
AB - Most in vitro functional and morphological studies for developing nervous system have been performed using traditional monolayer cultures onto supports modified by extracellular matrix components or synthetic biopolymers. These biomolecules act as adhesion factors essential for neuronal growth and differentiation. In this study, the use of chitosan as adhesion factor was investigated. Primary rat neurons and neurons differentiated from human induced pluripotent stem cells were cultured onto chitosan and standard adhesion factors modified supports. The initiation, elongation and branching of neuritic processes, synaptogenesis and electrophysiological behavior were studied. The biopolymers affected neurites outgrowth in a time dependent manner; in particular, chitosan promoted neuronal polarity in both cell cultures. These results indicate chitosan as a valid adhesion factor alternative to the standard ones, with the advantage that it can be used both in 2D and 3D cultures, acting as a bridge between these in vitro models.
KW - Axonal polarity
KW - Cell adhesion factors
KW - Chitosan
KW - Electrophysiological behavior
KW - Neurite outgrowth
KW - Neuronal cultures
KW - 2023 OA procedure
UR - http://www.scopus.com/inward/record.url?scp=85137022546&partnerID=8YFLogxK
U2 - 10.1016/j.carbpol.2022.120049
DO - 10.1016/j.carbpol.2022.120049
M3 - Article
C2 - 36184185
AN - SCOPUS:85137022546
VL - 297
JO - Carbohydrate polymers
JF - Carbohydrate polymers
SN - 0144-8617
M1 - 120049
ER -