One-pot squaric acid diester mediated aqueous protein conjugation

Frederik Wurm, Tobias Steinbach, Harm Anton Klok*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

30 Citations (Scopus)


A water-soluble squaric acid dialkyl diester derivative is presented, which enables one-pot, two-step amine-selective protein conjugation reactions with (functional) amines in water. This reagent not only allows all-aqueous protein modifications, but also tolerates e.g. hydroxyl groups and can also be used for the modification of proteins with water-insoluble amines.

Original languageEnglish
Pages (from-to)7815-7817
Number of pages3
JournalChemical communications
Issue number71
Publication statusPublished - 8 Aug 2013
Externally publishedYes


Dive into the research topics of 'One-pot squaric acid diester mediated aqueous protein conjugation'. Together they form a unique fingerprint.

Cite this