One-stage focal cartilage defect treatment with bone marrow mononuclear cells and chondrocytes leads to better macroscopic cartilage regeneration compared to microfracture in goats

J.E.J. Bekkers; L.B. Creemers; A.I. Tsuchida; M.H.P. van Rijen; R.J.H. Custers; W.J.A. Dhert; Daniël B.F. Saris

doi:10.1016/j.joca.2013.03.015

One-stage focal cartilage defect treatment with bone marrow mononuclear cells and chondrocytes leads to better macroscopic cartilage regeneration compared to microfracture in goats

J.E.J. Bekkers, L.B. Creemers, A.I. Tsuchida, M.H.P. van Rijen, R.J.H. Custers, W.J.A. Dhert, Daniël B.F. Saris

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Abstract

Objective The combination of chondrocytes and mononuclear fraction (MNF) cells might solve the expansion induced dedifferentiation problem of reimplanted cells in autologous chondrocytes implantation as sufficient cells would be available for direct, one-stage, implantation. Earlier in vitro work already showed a positive stimulation of cartilage specific matrix production when chondrocytes and MNF cells were combined. Therefore, this study aimed to evaluate cartilage regeneration using a one-stage procedure combining MNF cells and primary chondrocytes for the treatment of focal cartilage lesions in goats compared to microfracture treatment. Design Freshly created focal cartilage defects were treated with either a combination of chondrocytes and MNF cells embedded in fibrin glue or microfracture treatment. After 6 months follow-up local regeneration as well as the general joint cartilage health were evaluated using validated scores and biochemical assays. Results Macroscopic (p=0.015) scores for the cartilage surface at the treated defect were, after 6 months, significantly higher for the chondrocyteMNF treatment compared to microfracture-treated defects, but microscopic scores were not (p=0.067). The articulating cartilage showed more (p=0.005) degeneration following microfracture treatment compared to chondrocyte-MNF treatment. Biochemical GAG evaluation did not reveal differences between the treatments. Both treatments had resulted in a slight to moderate cartilage degeneration at other locations in the joint. Conclusion In conclusion, treatment of focal articular cartilage lesions in goats using a combination of mononuclear fraction cells from bone marrow and unexpanded chondrocytes leads to better macroscopic regeneration compared to microfracture, however needs further fine-tuning to decrease the negative influence on other joint compartments.

Original language	English
Pages (from-to)	950-956
Journal	Osteoarthritis and cartilage
Volume	21
Issue number	7
DOIs	https://doi.org/10.1016/j.joca.2013.03.015
Publication status	Published - 9 Apr 2013

Keywords

METIS-295688
IR-85465

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10.1016/j.joca.2013.03.015

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Bekkers, J. E. J., Creemers, L. B., Tsuchida, A. I., van Rijen, M. H. P., Custers, R. J. H., Dhert, W. J. A., & Saris, D. B. F. (2013). One-stage focal cartilage defect treatment with bone marrow mononuclear cells and chondrocytes leads to better macroscopic cartilage regeneration compared to microfracture in goats. Osteoarthritis and cartilage, 21(7), 950-956. https://doi.org/10.1016/j.joca.2013.03.015

@article{82d3d59bb4784b0c9eeda9dbf2167fbf,

title = "One-stage focal cartilage defect treatment with bone marrow mononuclear cells and chondrocytes leads to better macroscopic cartilage regeneration compared to microfracture in goats",

abstract = "Objective The combination of chondrocytes and mononuclear fraction (MNF) cells might solve the expansion induced dedifferentiation problem of reimplanted cells in autologous chondrocytes implantation as sufficient cells would be available for direct, one-stage, implantation. Earlier in vitro work already showed a positive stimulation of cartilage specific matrix production when chondrocytes and MNF cells were combined. Therefore, this study aimed to evaluate cartilage regeneration using a one-stage procedure combining MNF cells and primary chondrocytes for the treatment of focal cartilage lesions in goats compared to microfracture treatment. Design Freshly created focal cartilage defects were treated with either a combination of chondrocytes and MNF cells embedded in fibrin glue or microfracture treatment. After 6 months follow-up local regeneration as well as the general joint cartilage health were evaluated using validated scores and biochemical assays. Results Macroscopic (p=0.015) scores for the cartilage surface at the treated defect were, after 6 months, significantly higher for the chondrocyteMNF treatment compared to microfracture-treated defects, but microscopic scores were not (p=0.067). The articulating cartilage showed more (p=0.005) degeneration following microfracture treatment compared to chondrocyte-MNF treatment. Biochemical GAG evaluation did not reveal differences between the treatments. Both treatments had resulted in a slight to moderate cartilage degeneration at other locations in the joint. Conclusion In conclusion, treatment of focal articular cartilage lesions in goats using a combination of mononuclear fraction cells from bone marrow and unexpanded chondrocytes leads to better macroscopic regeneration compared to microfracture, however needs further fine-tuning to decrease the negative influence on other joint compartments.",

keywords = "METIS-295688, IR-85465",

author = "J.E.J. Bekkers and L.B. Creemers and A.I. Tsuchida and {van Rijen}, M.H.P. and R.J.H. Custers and W.J.A. Dhert and Saris, {Dani{\"e}l B.F.}",

note = "In Press, Accepted Manuscript ",

year = "2013",

month = apr,

day = "9",

doi = "10.1016/j.joca.2013.03.015",

language = "English",

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pages = "950--956",

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Bekkers, JEJ, Creemers, LB, Tsuchida, AI, van Rijen, MHP, Custers, RJH, Dhert, WJA & Saris, DBF 2013, 'One-stage focal cartilage defect treatment with bone marrow mononuclear cells and chondrocytes leads to better macroscopic cartilage regeneration compared to microfracture in goats', Osteoarthritis and cartilage, vol. 21, no. 7, pp. 950-956. https://doi.org/10.1016/j.joca.2013.03.015

One-stage focal cartilage defect treatment with bone marrow mononuclear cells and chondrocytes leads to better macroscopic cartilage regeneration compared to microfracture in goats. / Bekkers, J.E.J.; Creemers, L.B.; Tsuchida, A.I. et al.
In: Osteoarthritis and cartilage, Vol. 21, No. 7, 09.04.2013, p. 950-956.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - One-stage focal cartilage defect treatment with bone marrow mononuclear cells and chondrocytes leads to better macroscopic cartilage regeneration compared to microfracture in goats

AU - Bekkers, J.E.J.

AU - Creemers, L.B.

AU - Tsuchida, A.I.

AU - van Rijen, M.H.P.

AU - Custers, R.J.H.

AU - Dhert, W.J.A.

AU - Saris, Daniël B.F.

N1 - In Press, Accepted Manuscript

PY - 2013/4/9

Y1 - 2013/4/9

N2 - Objective The combination of chondrocytes and mononuclear fraction (MNF) cells might solve the expansion induced dedifferentiation problem of reimplanted cells in autologous chondrocytes implantation as sufficient cells would be available for direct, one-stage, implantation. Earlier in vitro work already showed a positive stimulation of cartilage specific matrix production when chondrocytes and MNF cells were combined. Therefore, this study aimed to evaluate cartilage regeneration using a one-stage procedure combining MNF cells and primary chondrocytes for the treatment of focal cartilage lesions in goats compared to microfracture treatment. Design Freshly created focal cartilage defects were treated with either a combination of chondrocytes and MNF cells embedded in fibrin glue or microfracture treatment. After 6 months follow-up local regeneration as well as the general joint cartilage health were evaluated using validated scores and biochemical assays. Results Macroscopic (p=0.015) scores for the cartilage surface at the treated defect were, after 6 months, significantly higher for the chondrocyteMNF treatment compared to microfracture-treated defects, but microscopic scores were not (p=0.067). The articulating cartilage showed more (p=0.005) degeneration following microfracture treatment compared to chondrocyte-MNF treatment. Biochemical GAG evaluation did not reveal differences between the treatments. Both treatments had resulted in a slight to moderate cartilage degeneration at other locations in the joint. Conclusion In conclusion, treatment of focal articular cartilage lesions in goats using a combination of mononuclear fraction cells from bone marrow and unexpanded chondrocytes leads to better macroscopic regeneration compared to microfracture, however needs further fine-tuning to decrease the negative influence on other joint compartments.

AB - Objective The combination of chondrocytes and mononuclear fraction (MNF) cells might solve the expansion induced dedifferentiation problem of reimplanted cells in autologous chondrocytes implantation as sufficient cells would be available for direct, one-stage, implantation. Earlier in vitro work already showed a positive stimulation of cartilage specific matrix production when chondrocytes and MNF cells were combined. Therefore, this study aimed to evaluate cartilage regeneration using a one-stage procedure combining MNF cells and primary chondrocytes for the treatment of focal cartilage lesions in goats compared to microfracture treatment. Design Freshly created focal cartilage defects were treated with either a combination of chondrocytes and MNF cells embedded in fibrin glue or microfracture treatment. After 6 months follow-up local regeneration as well as the general joint cartilage health were evaluated using validated scores and biochemical assays. Results Macroscopic (p=0.015) scores for the cartilage surface at the treated defect were, after 6 months, significantly higher for the chondrocyteMNF treatment compared to microfracture-treated defects, but microscopic scores were not (p=0.067). The articulating cartilage showed more (p=0.005) degeneration following microfracture treatment compared to chondrocyte-MNF treatment. Biochemical GAG evaluation did not reveal differences between the treatments. Both treatments had resulted in a slight to moderate cartilage degeneration at other locations in the joint. Conclusion In conclusion, treatment of focal articular cartilage lesions in goats using a combination of mononuclear fraction cells from bone marrow and unexpanded chondrocytes leads to better macroscopic regeneration compared to microfracture, however needs further fine-tuning to decrease the negative influence on other joint compartments.

KW - METIS-295688

KW - IR-85465

U2 - 10.1016/j.joca.2013.03.015

DO - 10.1016/j.joca.2013.03.015

M3 - Article

SN - 1063-4584

VL - 21

SP - 950

EP - 956

JO - Osteoarthritis and cartilage

JF - Osteoarthritis and cartilage

IS - 7

ER -

One-stage focal cartilage defect treatment with bone marrow mononuclear cells and chondrocytes leads to better macroscopic cartilage regeneration compared to microfracture in goats

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