One-step quantitative cortisol dipstick with proportional reading

Wingman Leung, Puiyee Chan, F. Bosgoed, Karin Lehmann, Ilka Renneberg, Matthias Lehmann, Reinhard Renneberg

Research output: Contribution to journalArticleAcademic

40 Citations (Scopus)

Abstract

Rapid quantitative immuno-detection of haptens by the lateral flow assay without “typical” competitive inhibition results is studied. In the present study, we describe an immuno-threshold-based assay for the quantification of cortisol. It gives a signal which is directly proportional to the cortisol concentration in plasma samples with a performance time of only 5 min. This technique provides a practical calibration curve with detection limit of 3.5 ng/ml. The precision of the assay is 6% (intra-assay coefficient of variation, CV) and 10% (inter-assay CV). Cross-reactivity with related steroids is acceptably low: corticosterone (3.38%), cortisone (2.08%), deoxycorticosterone (2.00%), 17α-hydroxyprogesterone (0.39%), and progesterone (0.05%). Furthermore, the test strips show the advantages of long storage time and high stability that allow mass production and preparation of large batches. A one-step cortisol whole blood test derived from this plasma lateral flow assay has then been performed. It is a rapid chromatographic immunoassay designed for quantitative determination of cortisol in whole blood samples. It requires no sample pretreatment and gives result within 15 min. In principle, with this rapid and sensitive immunoassay, the immuno-test strips can be employed for detecting all low-molecular-weight haptens. It may also be a useful and convenient dipstick format for drug detection.
Original languageEnglish
Pages (from-to)109-118
JournalJournal of immunological methods
Volume281
Issue number1-2
DOIs
Publication statusPublished - 2003

Fingerprint

Hydrocortisone
Reading
Haptens
Immunoassay
17-alpha-Hydroxyprogesterone
Desoxycorticosterone
Cortisone
Hematologic Tests
Corticosterone
Calibration
Progesterone
Limit of Detection
Molecular Weight
Steroids
Pharmaceutical Preparations

Keywords

  • One-step
  • IR-75025
  • Cortisol
  • Dipstick
  • Immuno-threshold

Cite this

Leung, W., Chan, P., Bosgoed, F., Lehmann, K., Renneberg, I., Lehmann, M., & Renneberg, R. (2003). One-step quantitative cortisol dipstick with proportional reading. Journal of immunological methods, 281(1-2), 109-118. https://doi.org/10.1016/j.jim.2003.07.009
Leung, Wingman ; Chan, Puiyee ; Bosgoed, F. ; Lehmann, Karin ; Renneberg, Ilka ; Lehmann, Matthias ; Renneberg, Reinhard. / One-step quantitative cortisol dipstick with proportional reading. In: Journal of immunological methods. 2003 ; Vol. 281, No. 1-2. pp. 109-118.
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Leung, W, Chan, P, Bosgoed, F, Lehmann, K, Renneberg, I, Lehmann, M & Renneberg, R 2003, 'One-step quantitative cortisol dipstick with proportional reading' Journal of immunological methods, vol. 281, no. 1-2, pp. 109-118. https://doi.org/10.1016/j.jim.2003.07.009

One-step quantitative cortisol dipstick with proportional reading. / Leung, Wingman; Chan, Puiyee; Bosgoed, F.; Lehmann, Karin; Renneberg, Ilka; Lehmann, Matthias; Renneberg, Reinhard.

In: Journal of immunological methods, Vol. 281, No. 1-2, 2003, p. 109-118.

Research output: Contribution to journalArticleAcademic

TY - JOUR

T1 - One-step quantitative cortisol dipstick with proportional reading

AU - Leung, Wingman

AU - Chan, Puiyee

AU - Bosgoed, F.

AU - Lehmann, Karin

AU - Renneberg, Ilka

AU - Lehmann, Matthias

AU - Renneberg, Reinhard

PY - 2003

Y1 - 2003

N2 - Rapid quantitative immuno-detection of haptens by the lateral flow assay without “typical” competitive inhibition results is studied. In the present study, we describe an immuno-threshold-based assay for the quantification of cortisol. It gives a signal which is directly proportional to the cortisol concentration in plasma samples with a performance time of only 5 min. This technique provides a practical calibration curve with detection limit of 3.5 ng/ml. The precision of the assay is 6% (intra-assay coefficient of variation, CV) and 10% (inter-assay CV). Cross-reactivity with related steroids is acceptably low: corticosterone (3.38%), cortisone (2.08%), deoxycorticosterone (2.00%), 17α-hydroxyprogesterone (0.39%), and progesterone (0.05%). Furthermore, the test strips show the advantages of long storage time and high stability that allow mass production and preparation of large batches. A one-step cortisol whole blood test derived from this plasma lateral flow assay has then been performed. It is a rapid chromatographic immunoassay designed for quantitative determination of cortisol in whole blood samples. It requires no sample pretreatment and gives result within 15 min. In principle, with this rapid and sensitive immunoassay, the immuno-test strips can be employed for detecting all low-molecular-weight haptens. It may also be a useful and convenient dipstick format for drug detection.

AB - Rapid quantitative immuno-detection of haptens by the lateral flow assay without “typical” competitive inhibition results is studied. In the present study, we describe an immuno-threshold-based assay for the quantification of cortisol. It gives a signal which is directly proportional to the cortisol concentration in plasma samples with a performance time of only 5 min. This technique provides a practical calibration curve with detection limit of 3.5 ng/ml. The precision of the assay is 6% (intra-assay coefficient of variation, CV) and 10% (inter-assay CV). Cross-reactivity with related steroids is acceptably low: corticosterone (3.38%), cortisone (2.08%), deoxycorticosterone (2.00%), 17α-hydroxyprogesterone (0.39%), and progesterone (0.05%). Furthermore, the test strips show the advantages of long storage time and high stability that allow mass production and preparation of large batches. A one-step cortisol whole blood test derived from this plasma lateral flow assay has then been performed. It is a rapid chromatographic immunoassay designed for quantitative determination of cortisol in whole blood samples. It requires no sample pretreatment and gives result within 15 min. In principle, with this rapid and sensitive immunoassay, the immuno-test strips can be employed for detecting all low-molecular-weight haptens. It may also be a useful and convenient dipstick format for drug detection.

KW - One-step

KW - IR-75025

KW - Cortisol

KW - Dipstick

KW - Immuno-threshold

U2 - 10.1016/j.jim.2003.07.009

DO - 10.1016/j.jim.2003.07.009

M3 - Article

VL - 281

SP - 109

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JO - Journal of immunological methods

JF - Journal of immunological methods

SN - 0022-1759

IS - 1-2

ER -

Leung W, Chan P, Bosgoed F, Lehmann K, Renneberg I, Lehmann M et al. One-step quantitative cortisol dipstick with proportional reading. Journal of immunological methods. 2003;281(1-2):109-118. https://doi.org/10.1016/j.jim.2003.07.009