Opening the ‘‘White Box’’ in Tissue Engineering: Visualization of Cell Aggregates in Optically Scattering Scaffolds

Milou Groot Nibbelink, Khalid Daoudi, S. Slegers, D.C. Grootendorst, Maura Dantuma, Wiendelt Steenbergen, Hermanus Bernardus Johannes Karperien, Srirang Manohar, Aart A. van Apeldoorn

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Abstract

The noninvasive and longitudinal imaging of cells or cell aggregates in large optically scattering scaffolds is still a largely unresolved problem in tissue engineering. In this work, we investigated the potential of near-infrared (NIR) photoacoustic (PA) tomography imaging to address this issue. We used clinically relevant sizes of highly light scattering polyethersulfone multibore hollow fiber scaffolds seeded with cells. Since cells have little optical absorption at NIR wavelengths, we studied labeling of cells with absorbers. Four NIR labels were examined for their suitability based on absorption characteristics, resistance to bleaching, and influence on cell viability. On the basis of these criteria, carbon nanoparticles proved most suitable in a variety of cells. For PA imaging, we used a research setup, based on computed tomography geometry. As proof of principle, using this imager we monitored the distribution and clustering of labeled rat insulinoma beta cell aggregates in the scaffolds. This was performed for the duration of 1 week in a nondestructive manner. The results were validated using fluorescence imaging, histology, and light microscopy imaging. Based on our findings, we conclude that PA tomography is a powerful tool for the nondestructive imaging of cells in optically scattering tissue-engineered scaffolds.
Original languageEnglish
Pages (from-to)534-542
JournalTissue engineering. Part C: Methods
Volume22
Issue number6
DOIs
Publication statusPublished - 8 Apr 2016

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Tissue Engineering
Tissue engineering
Scaffolds
Visualization
Scattering
Imaging techniques
Photoacoustic effect
Tomography
Infrared radiation
Tissue Scaffolds
Histology
Scaffolds (biology)
Bleaching
Light
Image sensors
Light scattering
Light absorption
Labeling
Insulinoma
Optical microscopy

Keywords

  • METIS-316963
  • IR-100460

Cite this

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title = "Opening the ‘‘White Box’’ in Tissue Engineering: Visualization of Cell Aggregates in Optically Scattering Scaffolds",
abstract = "The noninvasive and longitudinal imaging of cells or cell aggregates in large optically scattering scaffolds is still a largely unresolved problem in tissue engineering. In this work, we investigated the potential of near-infrared (NIR) photoacoustic (PA) tomography imaging to address this issue. We used clinically relevant sizes of highly light scattering polyethersulfone multibore hollow fiber scaffolds seeded with cells. Since cells have little optical absorption at NIR wavelengths, we studied labeling of cells with absorbers. Four NIR labels were examined for their suitability based on absorption characteristics, resistance to bleaching, and influence on cell viability. On the basis of these criteria, carbon nanoparticles proved most suitable in a variety of cells. For PA imaging, we used a research setup, based on computed tomography geometry. As proof of principle, using this imager we monitored the distribution and clustering of labeled rat insulinoma beta cell aggregates in the scaffolds. This was performed for the duration of 1 week in a nondestructive manner. The results were validated using fluorescence imaging, histology, and light microscopy imaging. Based on our findings, we conclude that PA tomography is a powerful tool for the nondestructive imaging of cells in optically scattering tissue-engineered scaffolds.",
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Opening the ‘‘White Box’’ in Tissue Engineering: Visualization of Cell Aggregates in Optically Scattering Scaffolds. / Groot Nibbelink, Milou; Daoudi, Khalid; Slegers, S.; Grootendorst, D.C.; Dantuma, Maura; Steenbergen, Wiendelt; Karperien, Hermanus Bernardus Johannes; Manohar, Srirang; van Apeldoorn, Aart A.

In: Tissue engineering. Part C: Methods, Vol. 22, No. 6, 08.04.2016, p. 534-542.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Opening the ‘‘White Box’’ in Tissue Engineering: Visualization of Cell Aggregates in Optically Scattering Scaffolds

AU - Groot Nibbelink, Milou

AU - Daoudi, Khalid

AU - Slegers, S.

AU - Grootendorst, D.C.

AU - Dantuma, Maura

AU - Steenbergen, Wiendelt

AU - Karperien, Hermanus Bernardus Johannes

AU - Manohar, Srirang

AU - van Apeldoorn, Aart A.

PY - 2016/4/8

Y1 - 2016/4/8

N2 - The noninvasive and longitudinal imaging of cells or cell aggregates in large optically scattering scaffolds is still a largely unresolved problem in tissue engineering. In this work, we investigated the potential of near-infrared (NIR) photoacoustic (PA) tomography imaging to address this issue. We used clinically relevant sizes of highly light scattering polyethersulfone multibore hollow fiber scaffolds seeded with cells. Since cells have little optical absorption at NIR wavelengths, we studied labeling of cells with absorbers. Four NIR labels were examined for their suitability based on absorption characteristics, resistance to bleaching, and influence on cell viability. On the basis of these criteria, carbon nanoparticles proved most suitable in a variety of cells. For PA imaging, we used a research setup, based on computed tomography geometry. As proof of principle, using this imager we monitored the distribution and clustering of labeled rat insulinoma beta cell aggregates in the scaffolds. This was performed for the duration of 1 week in a nondestructive manner. The results were validated using fluorescence imaging, histology, and light microscopy imaging. Based on our findings, we conclude that PA tomography is a powerful tool for the nondestructive imaging of cells in optically scattering tissue-engineered scaffolds.

AB - The noninvasive and longitudinal imaging of cells or cell aggregates in large optically scattering scaffolds is still a largely unresolved problem in tissue engineering. In this work, we investigated the potential of near-infrared (NIR) photoacoustic (PA) tomography imaging to address this issue. We used clinically relevant sizes of highly light scattering polyethersulfone multibore hollow fiber scaffolds seeded with cells. Since cells have little optical absorption at NIR wavelengths, we studied labeling of cells with absorbers. Four NIR labels were examined for their suitability based on absorption characteristics, resistance to bleaching, and influence on cell viability. On the basis of these criteria, carbon nanoparticles proved most suitable in a variety of cells. For PA imaging, we used a research setup, based on computed tomography geometry. As proof of principle, using this imager we monitored the distribution and clustering of labeled rat insulinoma beta cell aggregates in the scaffolds. This was performed for the duration of 1 week in a nondestructive manner. The results were validated using fluorescence imaging, histology, and light microscopy imaging. Based on our findings, we conclude that PA tomography is a powerful tool for the nondestructive imaging of cells in optically scattering tissue-engineered scaffolds.

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