Optical characterization of individual liposome-loaded microbubbles

Ying Luan, Telli Faez, Ilya Skachkov, Erik Gelderblom, Bart Geers, Ine Lentacker, Ton van der Steen, Michel Versluis, Nico de Jong

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Newly developed liposome-loaded (LPS) microbubbles are characterized by comparing their oscillating response with standard phospholipid-coated (bare) microbubbles using the ultra-high speed imaging (Brandaris 128) camera. A study of the shell properties indicate nearly the same shell elasticity and a higher shell viscosity for LPS bubbles than for bare bubbles. A frequency and pressure-dependent bubble acoustical behavior study shows a higher threshold for the initiation of bubble vibrations for LPS bubbles. In addition, an “expansion-only” behavior was observed for up to 69% of the investigated LPS bubbles which mostly occurred at lower acoustic pressures (≤30 kPa). Liposome attachment stability were studied using fluorescence imaging. The internal relationship among morphological structure, shell properties and ultrasonic behavior of LPS bubbles by optical characterization facilitate preclinical study and clinical application of LPS bubbles in ultrasound triggered drug delivery system.Newly developed liposome-loaded (LPS) microbubbles are characterized by comparing their oscillating response with standard phospholipid-coated (bare) microbubbles using the ultra-high speed imaging (Brandaris 128) camera. A study of the shell properties indicate nearly the same shell elasticity and a higher shell viscosity for LPS bubbles than for bare bubbles. A frequency and pressure-dependent bubble acoustical behavior study shows a higher threshold for the initiation of bubble vibrations for LPS bubbles. In addition, an “expansion-only” behavior was observed for up to 69% of the investigated LPS bubbles which mostly occurred at lower acoustic pressures (≤30 kPa). Liposome attachment stability were studied using fluorescence imaging. The internal relationship among morphological structure, shell properties and ultrasonic behavior of LPS bubbles by optical characterization facilitate preclinical study and clinical application of LPS bubbles in ultrasound triggered drug delivery system.
Original languageEnglish
Title of host publication2011 IEEE International Ultrasonics Symposium (IUS)
Subtitle of host publication18-21 Oct. 2011
Place of PublicationPiscataway, NJ
PublisherIEEE
Pages1890-1893
Number of pages4
ISBN (Electronic)978-1-4577-1252-4
ISBN (Print)978-1-4577-1253-1
DOIs
Publication statusPublished - 2012
EventIEEE International Ultrasonics Symposium, IUS 2011 - Orlando, United States
Duration: 18 Oct 201121 Oct 2011

Conference

ConferenceIEEE International Ultrasonics Symposium, IUS 2011
Abbreviated titleIUS
CountryUnited States
CityOrlando
Period18/10/1121/10/11

Fingerprint

bubbles
attachment
delivery
drugs
elastic properties
ultrasonics
cameras
high speed
viscosity
vibration
fluorescence
expansion
thresholds
acoustics

Keywords

  • Liposome-loaded microbubbles
  • Shell properties
  • Drug delivery

Cite this

Luan, Y., Faez, T., Skachkov, I., Gelderblom, E., Geers, B., Lentacker, I., ... de Jong, N. (2012). Optical characterization of individual liposome-loaded microbubbles. In 2011 IEEE International Ultrasonics Symposium (IUS): 18-21 Oct. 2011 (pp. 1890-1893). Piscataway, NJ: IEEE. https://doi.org/10.1109/ULTSYM.2011.0472
Luan, Ying ; Faez, Telli ; Skachkov, Ilya ; Gelderblom, Erik ; Geers, Bart ; Lentacker, Ine ; van der Steen, Ton ; Versluis, Michel ; de Jong, Nico. / Optical characterization of individual liposome-loaded microbubbles. 2011 IEEE International Ultrasonics Symposium (IUS): 18-21 Oct. 2011. Piscataway, NJ : IEEE, 2012. pp. 1890-1893
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title = "Optical characterization of individual liposome-loaded microbubbles",
abstract = "Newly developed liposome-loaded (LPS) microbubbles are characterized by comparing their oscillating response with standard phospholipid-coated (bare) microbubbles using the ultra-high speed imaging (Brandaris 128) camera. A study of the shell properties indicate nearly the same shell elasticity and a higher shell viscosity for LPS bubbles than for bare bubbles. A frequency and pressure-dependent bubble acoustical behavior study shows a higher threshold for the initiation of bubble vibrations for LPS bubbles. In addition, an “expansion-only” behavior was observed for up to 69{\%} of the investigated LPS bubbles which mostly occurred at lower acoustic pressures (≤30 kPa). Liposome attachment stability were studied using fluorescence imaging. The internal relationship among morphological structure, shell properties and ultrasonic behavior of LPS bubbles by optical characterization facilitate preclinical study and clinical application of LPS bubbles in ultrasound triggered drug delivery system.Newly developed liposome-loaded (LPS) microbubbles are characterized by comparing their oscillating response with standard phospholipid-coated (bare) microbubbles using the ultra-high speed imaging (Brandaris 128) camera. A study of the shell properties indicate nearly the same shell elasticity and a higher shell viscosity for LPS bubbles than for bare bubbles. A frequency and pressure-dependent bubble acoustical behavior study shows a higher threshold for the initiation of bubble vibrations for LPS bubbles. In addition, an “expansion-only” behavior was observed for up to 69{\%} of the investigated LPS bubbles which mostly occurred at lower acoustic pressures (≤30 kPa). Liposome attachment stability were studied using fluorescence imaging. The internal relationship among morphological structure, shell properties and ultrasonic behavior of LPS bubbles by optical characterization facilitate preclinical study and clinical application of LPS bubbles in ultrasound triggered drug delivery system.",
keywords = "Liposome-loaded microbubbles, Shell properties, Drug delivery",
author = "Ying Luan and Telli Faez and Ilya Skachkov and Erik Gelderblom and Bart Geers and Ine Lentacker and {van der Steen}, Ton and Michel Versluis and {de Jong}, Nico",
year = "2012",
doi = "10.1109/ULTSYM.2011.0472",
language = "English",
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pages = "1890--1893",
booktitle = "2011 IEEE International Ultrasonics Symposium (IUS)",
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Luan, Y, Faez, T, Skachkov, I, Gelderblom, E, Geers, B, Lentacker, I, van der Steen, T, Versluis, M & de Jong, N 2012, Optical characterization of individual liposome-loaded microbubbles. in 2011 IEEE International Ultrasonics Symposium (IUS): 18-21 Oct. 2011. IEEE, Piscataway, NJ, pp. 1890-1893, IEEE International Ultrasonics Symposium, IUS 2011, Orlando, United States, 18/10/11. https://doi.org/10.1109/ULTSYM.2011.0472

Optical characterization of individual liposome-loaded microbubbles. / Luan, Ying; Faez, Telli; Skachkov, Ilya; Gelderblom, Erik; Geers, Bart; Lentacker, Ine; van der Steen, Ton; Versluis, Michel; de Jong, Nico.

2011 IEEE International Ultrasonics Symposium (IUS): 18-21 Oct. 2011. Piscataway, NJ : IEEE, 2012. p. 1890-1893.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - Optical characterization of individual liposome-loaded microbubbles

AU - Luan, Ying

AU - Faez, Telli

AU - Skachkov, Ilya

AU - Gelderblom, Erik

AU - Geers, Bart

AU - Lentacker, Ine

AU - van der Steen, Ton

AU - Versluis, Michel

AU - de Jong, Nico

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N2 - Newly developed liposome-loaded (LPS) microbubbles are characterized by comparing their oscillating response with standard phospholipid-coated (bare) microbubbles using the ultra-high speed imaging (Brandaris 128) camera. A study of the shell properties indicate nearly the same shell elasticity and a higher shell viscosity for LPS bubbles than for bare bubbles. A frequency and pressure-dependent bubble acoustical behavior study shows a higher threshold for the initiation of bubble vibrations for LPS bubbles. In addition, an “expansion-only” behavior was observed for up to 69% of the investigated LPS bubbles which mostly occurred at lower acoustic pressures (≤30 kPa). Liposome attachment stability were studied using fluorescence imaging. The internal relationship among morphological structure, shell properties and ultrasonic behavior of LPS bubbles by optical characterization facilitate preclinical study and clinical application of LPS bubbles in ultrasound triggered drug delivery system.Newly developed liposome-loaded (LPS) microbubbles are characterized by comparing their oscillating response with standard phospholipid-coated (bare) microbubbles using the ultra-high speed imaging (Brandaris 128) camera. A study of the shell properties indicate nearly the same shell elasticity and a higher shell viscosity for LPS bubbles than for bare bubbles. A frequency and pressure-dependent bubble acoustical behavior study shows a higher threshold for the initiation of bubble vibrations for LPS bubbles. In addition, an “expansion-only” behavior was observed for up to 69% of the investigated LPS bubbles which mostly occurred at lower acoustic pressures (≤30 kPa). Liposome attachment stability were studied using fluorescence imaging. The internal relationship among morphological structure, shell properties and ultrasonic behavior of LPS bubbles by optical characterization facilitate preclinical study and clinical application of LPS bubbles in ultrasound triggered drug delivery system.

AB - Newly developed liposome-loaded (LPS) microbubbles are characterized by comparing their oscillating response with standard phospholipid-coated (bare) microbubbles using the ultra-high speed imaging (Brandaris 128) camera. A study of the shell properties indicate nearly the same shell elasticity and a higher shell viscosity for LPS bubbles than for bare bubbles. A frequency and pressure-dependent bubble acoustical behavior study shows a higher threshold for the initiation of bubble vibrations for LPS bubbles. In addition, an “expansion-only” behavior was observed for up to 69% of the investigated LPS bubbles which mostly occurred at lower acoustic pressures (≤30 kPa). Liposome attachment stability were studied using fluorescence imaging. The internal relationship among morphological structure, shell properties and ultrasonic behavior of LPS bubbles by optical characterization facilitate preclinical study and clinical application of LPS bubbles in ultrasound triggered drug delivery system.Newly developed liposome-loaded (LPS) microbubbles are characterized by comparing their oscillating response with standard phospholipid-coated (bare) microbubbles using the ultra-high speed imaging (Brandaris 128) camera. A study of the shell properties indicate nearly the same shell elasticity and a higher shell viscosity for LPS bubbles than for bare bubbles. A frequency and pressure-dependent bubble acoustical behavior study shows a higher threshold for the initiation of bubble vibrations for LPS bubbles. In addition, an “expansion-only” behavior was observed for up to 69% of the investigated LPS bubbles which mostly occurred at lower acoustic pressures (≤30 kPa). Liposome attachment stability were studied using fluorescence imaging. The internal relationship among morphological structure, shell properties and ultrasonic behavior of LPS bubbles by optical characterization facilitate preclinical study and clinical application of LPS bubbles in ultrasound triggered drug delivery system.

KW - Liposome-loaded microbubbles

KW - Shell properties

KW - Drug delivery

U2 - 10.1109/ULTSYM.2011.0472

DO - 10.1109/ULTSYM.2011.0472

M3 - Conference contribution

SN - 978-1-4577-1253-1

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BT - 2011 IEEE International Ultrasonics Symposium (IUS)

PB - IEEE

CY - Piscataway, NJ

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Luan Y, Faez T, Skachkov I, Gelderblom E, Geers B, Lentacker I et al. Optical characterization of individual liposome-loaded microbubbles. In 2011 IEEE International Ultrasonics Symposium (IUS): 18-21 Oct. 2011. Piscataway, NJ: IEEE. 2012. p. 1890-1893 https://doi.org/10.1109/ULTSYM.2011.0472