Optical characterization of individual liposome-loaded microbubbles

Ying Luan, Telli Faez, Ilya Skachkov, Erik Gelderblom, Bart Geers, Ine Lentacker, Ton van der Steen, Michel Versluis, Nico de Jong

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Newly developed liposome-loaded (LPS) microbubbles are characterized by comparing their oscillating response with standard phospholipid-coated (bare) microbubbles using the ultra-high speed imaging (Brandaris 128) camera. A study of the shell properties indicate nearly the same shell elasticity and a higher shell viscosity for LPS bubbles than for bare bubbles. A frequency and pressure-dependent bubble acoustical behavior study shows a higher threshold for the initiation of bubble vibrations for LPS bubbles. In addition, an “expansion-only” behavior was observed for up to 69% of the investigated LPS bubbles which mostly occurred at lower acoustic pressures (≤30 kPa). Liposome attachment stability were studied using fluorescence imaging. The internal relationship among morphological structure, shell properties and ultrasonic behavior of LPS bubbles by optical characterization facilitate preclinical study and clinical application of LPS bubbles in ultrasound triggered drug delivery system.Newly developed liposome-loaded (LPS) microbubbles are characterized by comparing their oscillating response with standard phospholipid-coated (bare) microbubbles using the ultra-high speed imaging (Brandaris 128) camera. A study of the shell properties indicate nearly the same shell elasticity and a higher shell viscosity for LPS bubbles than for bare bubbles. A frequency and pressure-dependent bubble acoustical behavior study shows a higher threshold for the initiation of bubble vibrations for LPS bubbles. In addition, an “expansion-only” behavior was observed for up to 69% of the investigated LPS bubbles which mostly occurred at lower acoustic pressures (≤30 kPa). Liposome attachment stability were studied using fluorescence imaging. The internal relationship among morphological structure, shell properties and ultrasonic behavior of LPS bubbles by optical characterization facilitate preclinical study and clinical application of LPS bubbles in ultrasound triggered drug delivery system.
Original languageEnglish
Title of host publication2011 IEEE International Ultrasonics Symposium (IUS)
Subtitle of host publication18-21 Oct. 2011
Place of PublicationPiscataway, NJ
PublisherIEEE
Pages1890-1893
Number of pages4
ISBN (Electronic)978-1-4577-1252-4
ISBN (Print)978-1-4577-1253-1
DOIs
Publication statusPublished - 2012
EventIEEE International Ultrasonics Symposium, IUS 2011 - Orlando, United States
Duration: 18 Oct 201121 Oct 2011

Conference

ConferenceIEEE International Ultrasonics Symposium, IUS 2011
Abbreviated titleIUS
CountryUnited States
CityOrlando
Period18/10/1121/10/11

Keywords

  • Liposome-loaded microbubbles
  • Shell properties
  • Drug delivery

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  • Cite this

    Luan, Y., Faez, T., Skachkov, I., Gelderblom, E., Geers, B., Lentacker, I., ... de Jong, N. (2012). Optical characterization of individual liposome-loaded microbubbles. In 2011 IEEE International Ultrasonics Symposium (IUS): 18-21 Oct. 2011 (pp. 1890-1893). Piscataway, NJ: IEEE. https://doi.org/10.1109/ULTSYM.2011.0472