Orented immobilization of farnesylated proteins by the thiol-ene reaction

Dirk Weinrich; Po-Chiao Lin; Pascal Jonkheijm; Uyen T.T. Nguyen; Hendrik Schröder; Christof M. Niemeyer; Kirill Alexandrov; Roger Goody; Herbert Waldmann

doi:10.1002/anie.200906190

Orented immobilization of farnesylated proteins by the thiol-ene reaction

Dirk Weinrich, Po-Chiao Lin, Pascal Jonkheijm, Uyen T.T. Nguyen, Hendrik Schröder, Christof M. Niemeyer, Kirill Alexandrov, Roger Goody, Herbert Waldmann

Research output: Contribution to journal › Article › Academic › peer-review

80 Citations (Scopus)

Abstract

Anchoring the protein: Proteins were immobilized rapidly under mild conditions by thiol-ene photocoupling between S-farnesyl groups attached to a genetically encodable “CAAX-box” tetrapeptide sequence (A is aliphatic) at the C terminus of the protein and surface-exposed thiols (see scheme). This method enables the oriented covalent immobilization of proteins directly from expression lysates without additional purification or derivatization steps.

Original language	English
Pages (from-to)	1252-1257
Number of pages	6
Journal	Angewandte Chemie, International Edition in English
Volume	49
Issue number	7
DOIs	https://doi.org/10.1002/anie.200906190
Publication status	Published - 2010

Keywords

IR-76904
immobilization
protein microarrays
Farnesylation
METIS-273437
thiol-ene reaction
photochemical reactions

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10.1002/anie.200906190

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title = "Orented immobilization of farnesylated proteins by the thiol-ene reaction",

abstract = "Anchoring the protein: Proteins were immobilized rapidly under mild conditions by thiol-ene photocoupling between S-farnesyl groups attached to a genetically encodable “CAAX-box” tetrapeptide sequence (A is aliphatic) at the C terminus of the protein and surface-exposed thiols (see scheme). This method enables the oriented covalent immobilization of proteins directly from expression lysates without additional purification or derivatization steps.",

keywords = "IR-76904, immobilization, protein microarrays, Farnesylation, METIS-273437, thiol-ene reaction, photochemical reactions",

author = "Dirk Weinrich and Po-Chiao Lin and Pascal Jonkheijm and Nguyen, {Uyen T.T.} and Hendrik Schr{\"o}der and Niemeyer, {Christof M.} and Kirill Alexandrov and Roger Goody and Herbert Waldmann",

year = "2010",

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volume = "49",

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Orented immobilization of farnesylated proteins by the thiol-ene reaction. / Weinrich, Dirk; Lin, Po-Chiao; Jonkheijm, Pascal; Nguyen, Uyen T.T.; Schröder, Hendrik; Niemeyer, Christof M.; Alexandrov, Kirill; Goody, Roger; Waldmann, Herbert.

In: Angewandte Chemie, International Edition in English, Vol. 49, No. 7, 2010, p. 1252-1257.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Orented immobilization of farnesylated proteins by the thiol-ene reaction

AU - Weinrich, Dirk

AU - Lin, Po-Chiao

AU - Jonkheijm, Pascal

AU - Nguyen, Uyen T.T.

AU - Schröder, Hendrik

AU - Niemeyer, Christof M.

AU - Alexandrov, Kirill

AU - Goody, Roger

AU - Waldmann, Herbert

PY - 2010

Y1 - 2010

N2 - Anchoring the protein: Proteins were immobilized rapidly under mild conditions by thiol-ene photocoupling between S-farnesyl groups attached to a genetically encodable “CAAX-box” tetrapeptide sequence (A is aliphatic) at the C terminus of the protein and surface-exposed thiols (see scheme). This method enables the oriented covalent immobilization of proteins directly from expression lysates without additional purification or derivatization steps.

AB - Anchoring the protein: Proteins were immobilized rapidly under mild conditions by thiol-ene photocoupling between S-farnesyl groups attached to a genetically encodable “CAAX-box” tetrapeptide sequence (A is aliphatic) at the C terminus of the protein and surface-exposed thiols (see scheme). This method enables the oriented covalent immobilization of proteins directly from expression lysates without additional purification or derivatization steps.

KW - IR-76904

KW - immobilization

KW - protein microarrays

KW - Farnesylation

KW - METIS-273437

KW - thiol-ene reaction

KW - photochemical reactions

U2 - 10.1002/anie.200906190

DO - 10.1002/anie.200906190

M3 - Article

VL - 49

SP - 1252

EP - 1257

JO - Angewandte Chemie (international edition)

JF - Angewandte Chemie (international edition)

SN - 1433-7851

IS - 7

ER -

Orented immobilization of farnesylated proteins by the thiol-ene reaction

Abstract

Keywords

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