Abstract
Anchoring the protein: Proteins were immobilized rapidly under mild conditions by thiol-ene photocoupling between S-farnesyl groups attached to a genetically encodable “CAAX-box” tetrapeptide sequence (A is aliphatic) at the C terminus of the protein and surface-exposed thiols (see scheme). This method enables the oriented covalent immobilization of proteins directly from expression lysates without additional purification or derivatization steps.
Original language | English |
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Pages (from-to) | 1252-1257 |
Number of pages | 6 |
Journal | Angewandte Chemie, International Edition in English |
Volume | 49 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2010 |
Keywords
- IR-76904
- immobilization
- protein microarrays
- Farnesylation
- METIS-273437
- thiol-ene reaction
- photochemical reactions