Orented immobilization of farnesylated proteins by the thiol-ene reaction

Dirk Weinrich, Po-Chiao Lin, Pascal Jonkheijm, Uyen T.T. Nguyen, Hendrik Schröder, Christof M. Niemeyer, Kirill Alexandrov, Roger Goody, Herbert Waldmann

Research output: Contribution to journalArticleAcademicpeer-review

77 Citations (Scopus)

Abstract

Anchoring the protein: Proteins were immobilized rapidly under mild conditions by thiol-ene photocoupling between S-farnesyl groups attached to a genetically encodable “CAAX-box” tetrapeptide sequence (A is aliphatic) at the C terminus of the protein and surface-exposed thiols (see scheme). This method enables the oriented covalent immobilization of proteins directly from expression lysates without additional purification or derivatization steps.
Original languageEnglish
Pages (from-to)1252-1257
Number of pages6
JournalAngewandte Chemie, International Edition in English
Volume49
Issue number7
DOIs
Publication statusPublished - 2010

Keywords

  • IR-76904
  • immobilization
  • protein microarrays
  • Farnesylation
  • METIS-273437
  • thiol-ene reaction
  • photochemical reactions

Fingerprint Dive into the research topics of 'Orented immobilization of farnesylated proteins by the thiol-ene reaction'. Together they form a unique fingerprint.

Cite this