Anchoring the protein: Proteins were immobilized rapidly under mild conditions by thiol-ene photocoupling between S-farnesyl groups attached to a genetically encodable “CAAX-box” tetrapeptide sequence (A is aliphatic) at the C terminus of the protein and surface-exposed thiols (see scheme). This method enables the oriented covalent immobilization of proteins directly from expression lysates without additional purification or derivatization steps.
|Number of pages||6|
|Journal||Angewandte Chemie, International Edition in English|
|Publication status||Published - 2010|
- protein microarrays
- thiol-ene reaction
- photochemical reactions