Orthogonal supramolecular protein assembly on patterned bifunctional surfaces

D. Wasserberg; J. Cabanas-Danés; V. Subramaniam; J. Huskens; P. Jonkheijm

doi:10.1039/c7cc09808a

Orthogonal supramolecular protein assembly on patterned bifunctional surfaces

D. Wasserberg, J. Cabanas-Danés, V. Subramaniam, J. Huskens, P. Jonkheijm^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

We report successful and selective dual protein assembly on patterned bifunctional βCD-Ni(ii)NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.

Original language	English
Pages (from-to)	1615-1618
Number of pages	4
Journal	Chemical communications
Volume	54
Issue number	13
DOIs	https://doi.org/10.1039/c7cc09808a
Publication status	Published - 19 Jan 2018

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title = "Orthogonal supramolecular protein assembly on patterned bifunctional surfaces",

abstract = "We report successful and selective dual protein assembly on patterned bifunctional βCD-Ni(ii)NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.",

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AU - Cabanas-Danés, J.

AU - Subramaniam, V.

AU - Huskens, J.

AU - Jonkheijm, P.

PY - 2018/1/19

Y1 - 2018/1/19

N2 - We report successful and selective dual protein assembly on patterned bifunctional βCD-Ni(ii)NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.

AB - We report successful and selective dual protein assembly on patterned bifunctional βCD-Ni(ii)NTA surfaces, using red fluorescent protein variants with hexahistidine-tags and teal fluorescent protein variants conjugated with a peptide containing three adamantyl groups. We show that dual protein patterns can only be assembled, when opposing supramolecular interactions have been optimized and nonspecific interactions have been sufficiently suppressed.

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DO - 10.1039/c7cc09808a

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Orthogonal supramolecular protein assembly on patterned bifunctional surfaces

Abstract

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