TY - JOUR
T1 - Osteogenic induction of adipose-derived stromal cells
T2 - not a requirement for bone formation in vivo
AU - Li, Xiaoyu
AU - Yao, Jinfeng
AU - Wu, Ling
AU - Jing, Wei
AU - Tang, Wei
AU - Lin, Yunfeng
AU - Tian, Weidong
AU - Liu, Lei
PY - 2010
Y1 - 2010
N2 - Osteogenic induction was regarded as an indispensable step for adipose‐derived stromal cells (ADSCs) to have osteogenic ability. Non‐induced ADSCs can also produce bone in vivo and heal skeletal defects. The present study aimed to compare the bone‐forming ability of osteogenically induced ADSCs and non‐induced ADSCs in vivo. Tissue‐engineered constructs were prepared from osteogenically induced or non‐induced ADSCs and porous hydroxyapatite/beta‐tricalcium phosphate scaffolds. A scaffold without cells and an empty defect group were used as control. All were implanted in rat critical calvarial defects. After implantation for 6 and 12 weeks, bone formation was analyzed using histomorphometry and microcomputed tomography; there were no significant differences in the formation of new bone between osteogenically induced ADSCs and non‐induced ADSCs (P>0.05). In conclusion, osteogenic induction of ADSCs is not an indispensable step for bone formation in vivo. Non‐induced ADSCs can also be used as seeding cells to construct bone tissue.
AB - Osteogenic induction was regarded as an indispensable step for adipose‐derived stromal cells (ADSCs) to have osteogenic ability. Non‐induced ADSCs can also produce bone in vivo and heal skeletal defects. The present study aimed to compare the bone‐forming ability of osteogenically induced ADSCs and non‐induced ADSCs in vivo. Tissue‐engineered constructs were prepared from osteogenically induced or non‐induced ADSCs and porous hydroxyapatite/beta‐tricalcium phosphate scaffolds. A scaffold without cells and an empty defect group were used as control. All were implanted in rat critical calvarial defects. After implantation for 6 and 12 weeks, bone formation was analyzed using histomorphometry and microcomputed tomography; there were no significant differences in the formation of new bone between osteogenically induced ADSCs and non‐induced ADSCs (P>0.05). In conclusion, osteogenic induction of ADSCs is not an indispensable step for bone formation in vivo. Non‐induced ADSCs can also be used as seeding cells to construct bone tissue.
U2 - 10.1111/j.1525-1594.2009.00795.x
DO - 10.1111/j.1525-1594.2009.00795.x
M3 - Article
SN - 0391-3988
VL - 34
SP - 46
EP - 54
JO - The International journal of artificial organs
JF - The International journal of artificial organs
IS - 1
ER -