Osteogenic magnesium incorporated into PLGA/TCP porous scaffold by 3D printing for repairing challenging bone defect

Yuxiao Lai (Corresponding Author), Ye Li, Huijuan Cao, Jing Long, Xinluan Wang, Long Li, Cairong Li, Qingyun Jia, Bin Teng, Tingting Tang, Jiang Peng, David Eglin, Mauro Alini, Dirk W. Grijpma, Geoff Richards, Ling Qin (Corresponding Author)

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Bone defect repair is a challenging clinical problem in musculoskeletal system, especially in orthopaedic disorders such as steroid associated osteonecrosis (SAON). Magnesium (Mg) as a biodegradable metal with properly mechanical properties has been investigating for a long history. In this study, Mg powder, poly (lactide-co-glycolide) (PLGA), β-tricalcium phosphate (β-TCP) were the elements to formulate a novel porous PLGA/TCP/Mg (PTM) scaffolds using low temperature rapid prototyping (LT-RP) technology. The physical characterization of PTM scaffold and Mg ions release were analyzed in vitro. The osteogenic and angiogenic properties of PTM scaffolds, as well as the biosafety after implantation were assessed in an established SAON rabbit model. Our results showed that the PTM scaffold possessed well-designed bio-mimic structure and improved mechanical properties. Findings of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and micro-computed tomography (micro CT)-based angiography indicated that PTM scaffold could increase blood perfusion and promote new vessel ingrowth at 4 weeks after surgery, meanwhile, a plenty of newly formed vessels with well-architective structure were observed at 8 weeks. Correspondingly, at 12 weeks after surgery, micro-CT, histological and mechanical properties analysis showed that PTM could significant enhance new bone formation and strengthen newly formed bone mechanical properties. The mean bone volume in PTM group was 56.3% greater than that in PT group. Biosafety assessments from 0 to 12 weeks after implantation did not induce increase in serum Mg ions concentration, and immune response, liver and kidney function parameters were all at normal level. These findings suggested that the PTM scaffold had both osteogenic and angiogenic abilities which were synergistic effect in enhancing new bone formation and strengthen newly formed bone quality in SAON. In summary, PTM scaffolds are promising composite biomaterials for repairing challenging bone defect that would have great potential for its clinical translation.

Original languageEnglish
Pages (from-to)207-219
Number of pages13
JournalBiomaterials
Volume197
DOIs
Publication statusPublished - 1 Mar 2019

Fingerprint

Scaffolds
Magnesium
Printing
Bone
Bone and Bones
Defects
Mechanical properties
Osteonecrosis
Surgery
Steroids
Magnesium powder
Musculoskeletal system
Osteogenesis
Angiography
Ions
Orthopedics
Rapid prototyping
Magnetic resonance
polylactic acid-polyglycolic acid copolymer
Three Dimensional Printing

Keywords

  • Angiogenesis
  • Biosafety
  • Osteogenesis
  • PLGA/TCP/Mg (PTM)
  • Steroid associated osteonecrosis (SAON)

Cite this

Lai, Yuxiao ; Li, Ye ; Cao, Huijuan ; Long, Jing ; Wang, Xinluan ; Li, Long ; Li, Cairong ; Jia, Qingyun ; Teng, Bin ; Tang, Tingting ; Peng, Jiang ; Eglin, David ; Alini, Mauro ; Grijpma, Dirk W. ; Richards, Geoff ; Qin, Ling. / Osteogenic magnesium incorporated into PLGA/TCP porous scaffold by 3D printing for repairing challenging bone defect. In: Biomaterials. 2019 ; Vol. 197. pp. 207-219.
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abstract = "Bone defect repair is a challenging clinical problem in musculoskeletal system, especially in orthopaedic disorders such as steroid associated osteonecrosis (SAON). Magnesium (Mg) as a biodegradable metal with properly mechanical properties has been investigating for a long history. In this study, Mg powder, poly (lactide-co-glycolide) (PLGA), β-tricalcium phosphate (β-TCP) were the elements to formulate a novel porous PLGA/TCP/Mg (PTM) scaffolds using low temperature rapid prototyping (LT-RP) technology. The physical characterization of PTM scaffold and Mg ions release were analyzed in vitro. The osteogenic and angiogenic properties of PTM scaffolds, as well as the biosafety after implantation were assessed in an established SAON rabbit model. Our results showed that the PTM scaffold possessed well-designed bio-mimic structure and improved mechanical properties. Findings of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and micro-computed tomography (micro CT)-based angiography indicated that PTM scaffold could increase blood perfusion and promote new vessel ingrowth at 4 weeks after surgery, meanwhile, a plenty of newly formed vessels with well-architective structure were observed at 8 weeks. Correspondingly, at 12 weeks after surgery, micro-CT, histological and mechanical properties analysis showed that PTM could significant enhance new bone formation and strengthen newly formed bone mechanical properties. The mean bone volume in PTM group was 56.3{\%} greater than that in PT group. Biosafety assessments from 0 to 12 weeks after implantation did not induce increase in serum Mg ions concentration, and immune response, liver and kidney function parameters were all at normal level. These findings suggested that the PTM scaffold had both osteogenic and angiogenic abilities which were synergistic effect in enhancing new bone formation and strengthen newly formed bone quality in SAON. In summary, PTM scaffolds are promising composite biomaterials for repairing challenging bone defect that would have great potential for its clinical translation.",
keywords = "Angiogenesis, Biosafety, Osteogenesis, PLGA/TCP/Mg (PTM), Steroid associated osteonecrosis (SAON)",
author = "Yuxiao Lai and Ye Li and Huijuan Cao and Jing Long and Xinluan Wang and Long Li and Cairong Li and Qingyun Jia and Bin Teng and Tingting Tang and Jiang Peng and David Eglin and Mauro Alini and Grijpma, {Dirk W.} and Geoff Richards and Ling Qin",
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Lai, Y, Li, Y, Cao, H, Long, J, Wang, X, Li, L, Li, C, Jia, Q, Teng, B, Tang, T, Peng, J, Eglin, D, Alini, M, Grijpma, DW, Richards, G & Qin, L 2019, 'Osteogenic magnesium incorporated into PLGA/TCP porous scaffold by 3D printing for repairing challenging bone defect' Biomaterials, vol. 197, pp. 207-219. https://doi.org/10.1016/j.biomaterials.2019.01.013

Osteogenic magnesium incorporated into PLGA/TCP porous scaffold by 3D printing for repairing challenging bone defect. / Lai, Yuxiao (Corresponding Author); Li, Ye; Cao, Huijuan; Long, Jing; Wang, Xinluan; Li, Long; Li, Cairong; Jia, Qingyun; Teng, Bin; Tang, Tingting; Peng, Jiang; Eglin, David; Alini, Mauro; Grijpma, Dirk W.; Richards, Geoff; Qin, Ling (Corresponding Author).

In: Biomaterials, Vol. 197, 01.03.2019, p. 207-219.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Osteogenic magnesium incorporated into PLGA/TCP porous scaffold by 3D printing for repairing challenging bone defect

AU - Lai, Yuxiao

AU - Li, Ye

AU - Cao, Huijuan

AU - Long, Jing

AU - Wang, Xinluan

AU - Li, Long

AU - Li, Cairong

AU - Jia, Qingyun

AU - Teng, Bin

AU - Tang, Tingting

AU - Peng, Jiang

AU - Eglin, David

AU - Alini, Mauro

AU - Grijpma, Dirk W.

AU - Richards, Geoff

AU - Qin, Ling

PY - 2019/3/1

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N2 - Bone defect repair is a challenging clinical problem in musculoskeletal system, especially in orthopaedic disorders such as steroid associated osteonecrosis (SAON). Magnesium (Mg) as a biodegradable metal with properly mechanical properties has been investigating for a long history. In this study, Mg powder, poly (lactide-co-glycolide) (PLGA), β-tricalcium phosphate (β-TCP) were the elements to formulate a novel porous PLGA/TCP/Mg (PTM) scaffolds using low temperature rapid prototyping (LT-RP) technology. The physical characterization of PTM scaffold and Mg ions release were analyzed in vitro. The osteogenic and angiogenic properties of PTM scaffolds, as well as the biosafety after implantation were assessed in an established SAON rabbit model. Our results showed that the PTM scaffold possessed well-designed bio-mimic structure and improved mechanical properties. Findings of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and micro-computed tomography (micro CT)-based angiography indicated that PTM scaffold could increase blood perfusion and promote new vessel ingrowth at 4 weeks after surgery, meanwhile, a plenty of newly formed vessels with well-architective structure were observed at 8 weeks. Correspondingly, at 12 weeks after surgery, micro-CT, histological and mechanical properties analysis showed that PTM could significant enhance new bone formation and strengthen newly formed bone mechanical properties. The mean bone volume in PTM group was 56.3% greater than that in PT group. Biosafety assessments from 0 to 12 weeks after implantation did not induce increase in serum Mg ions concentration, and immune response, liver and kidney function parameters were all at normal level. These findings suggested that the PTM scaffold had both osteogenic and angiogenic abilities which were synergistic effect in enhancing new bone formation and strengthen newly formed bone quality in SAON. In summary, PTM scaffolds are promising composite biomaterials for repairing challenging bone defect that would have great potential for its clinical translation.

AB - Bone defect repair is a challenging clinical problem in musculoskeletal system, especially in orthopaedic disorders such as steroid associated osteonecrosis (SAON). Magnesium (Mg) as a biodegradable metal with properly mechanical properties has been investigating for a long history. In this study, Mg powder, poly (lactide-co-glycolide) (PLGA), β-tricalcium phosphate (β-TCP) were the elements to formulate a novel porous PLGA/TCP/Mg (PTM) scaffolds using low temperature rapid prototyping (LT-RP) technology. The physical characterization of PTM scaffold and Mg ions release were analyzed in vitro. The osteogenic and angiogenic properties of PTM scaffolds, as well as the biosafety after implantation were assessed in an established SAON rabbit model. Our results showed that the PTM scaffold possessed well-designed bio-mimic structure and improved mechanical properties. Findings of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) and micro-computed tomography (micro CT)-based angiography indicated that PTM scaffold could increase blood perfusion and promote new vessel ingrowth at 4 weeks after surgery, meanwhile, a plenty of newly formed vessels with well-architective structure were observed at 8 weeks. Correspondingly, at 12 weeks after surgery, micro-CT, histological and mechanical properties analysis showed that PTM could significant enhance new bone formation and strengthen newly formed bone mechanical properties. The mean bone volume in PTM group was 56.3% greater than that in PT group. Biosafety assessments from 0 to 12 weeks after implantation did not induce increase in serum Mg ions concentration, and immune response, liver and kidney function parameters were all at normal level. These findings suggested that the PTM scaffold had both osteogenic and angiogenic abilities which were synergistic effect in enhancing new bone formation and strengthen newly formed bone quality in SAON. In summary, PTM scaffolds are promising composite biomaterials for repairing challenging bone defect that would have great potential for its clinical translation.

KW - Angiogenesis

KW - Biosafety

KW - Osteogenesis

KW - PLGA/TCP/Mg (PTM)

KW - Steroid associated osteonecrosis (SAON)

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