Oxygen gradients in tissue-engineered PEGT/PBT cartilaginous constructs: Measurement and modeling

J. Malda, Jeroen Rouwkema, D.E. Martens, EP le Comte, F.K. Kooy, J. Tramper, Clemens van Blitterswijk, J.U. Riesle

Research output: Contribution to journalArticleAcademicpeer-review

224 Citations (Scopus)

Abstract

The supply of oxygen within three-dimensional tissue-engineered (TE) cartilage polymer constructs is mainly by diffusion. Oxygen consumption by cells results in gradients in the oxygen concentration. The aims of this study were, firstly, to identify the gradients within TE cartilage polymer constructs and, secondly, to predict the profiles during in vitro culture. A glass microelectrode system was adapted and used to penetrate cartilage and TE cartilaginous constructs, yielding reproducible measurements with high spatial resolution. Cartilage polymer constructs were cultured for up to 41 days in vitro. Oxygen concentrations, as low as 2-5%, were measured within the center of these constructs. At the beginning of in vitro culture, the oxygen gradients were steeper in TE constructs in comparison to native tissue. Nevertheless, during the course of culture, oxygen concentrations approached the values measured in native tissue. A mathematical model was developed which yields oxygen profiles within cartilage explants and TE constructs. Model input parameters were assessed, including the diffusion coefficient of cartilage (2.2 × 10-9) + (0.4 × 10-9 m2 s-1), 70% of the diffusion coefficient of water and the diffusion coefficient of constructs (3.8 × 10-10 m2 s-1). The model confirmed that chondrocytes in polymer constructs cultured for 27 days have low oxygen requirements (0.8 × 10-19 mol m-3 s-1), even lower than chondrocytes in native cartilage. The ability to measure and predict local oxygen tensions offers new opportunities to obtain more insight in the relation between oxygen tension and chondrogenesis.
Original languageUndefined
Pages (from-to)9-18
JournalBiotechnology and bioengineering
Volume86
Issue number1
DOIs
Publication statusPublished - 2004

Keywords

  • oxygen gradient
  • Tissue Engineering
  • METIS-237077
  • Modeling
  • Cartilage
  • Chondrocyte
  • IR-72009

Cite this

Malda, J. ; Rouwkema, Jeroen ; Martens, D.E. ; le Comte, EP ; Kooy, F.K. ; Tramper, J. ; van Blitterswijk, Clemens ; Riesle, J.U. / Oxygen gradients in tissue-engineered PEGT/PBT cartilaginous constructs: Measurement and modeling. In: Biotechnology and bioengineering. 2004 ; Vol. 86, No. 1. pp. 9-18.
@article{4bbaf4a583a640fca2619d16db6aab55,
title = "Oxygen gradients in tissue-engineered PEGT/PBT cartilaginous constructs: Measurement and modeling",
abstract = "The supply of oxygen within three-dimensional tissue-engineered (TE) cartilage polymer constructs is mainly by diffusion. Oxygen consumption by cells results in gradients in the oxygen concentration. The aims of this study were, firstly, to identify the gradients within TE cartilage polymer constructs and, secondly, to predict the profiles during in vitro culture. A glass microelectrode system was adapted and used to penetrate cartilage and TE cartilaginous constructs, yielding reproducible measurements with high spatial resolution. Cartilage polymer constructs were cultured for up to 41 days in vitro. Oxygen concentrations, as low as 2-5{\%}, were measured within the center of these constructs. At the beginning of in vitro culture, the oxygen gradients were steeper in TE constructs in comparison to native tissue. Nevertheless, during the course of culture, oxygen concentrations approached the values measured in native tissue. A mathematical model was developed which yields oxygen profiles within cartilage explants and TE constructs. Model input parameters were assessed, including the diffusion coefficient of cartilage (2.2 × 10-9) + (0.4 × 10-9 m2 s-1), 70{\%} of the diffusion coefficient of water and the diffusion coefficient of constructs (3.8 × 10-10 m2 s-1). The model confirmed that chondrocytes in polymer constructs cultured for 27 days have low oxygen requirements (0.8 × 10-19 mol m-3 s-1), even lower than chondrocytes in native cartilage. The ability to measure and predict local oxygen tensions offers new opportunities to obtain more insight in the relation between oxygen tension and chondrogenesis.",
keywords = "oxygen gradient, Tissue Engineering, METIS-237077, Modeling, Cartilage, Chondrocyte, IR-72009",
author = "J. Malda and Jeroen Rouwkema and D.E. Martens and {le Comte}, EP and F.K. Kooy and J. Tramper and {van Blitterswijk}, Clemens and J.U. Riesle",
year = "2004",
doi = "10.1002/bit.20038",
language = "Undefined",
volume = "86",
pages = "9--18",
journal = "Biotechnology and bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "1",

}

Malda, J, Rouwkema, J, Martens, DE, le Comte, EP, Kooy, FK, Tramper, J, van Blitterswijk, C & Riesle, JU 2004, 'Oxygen gradients in tissue-engineered PEGT/PBT cartilaginous constructs: Measurement and modeling' Biotechnology and bioengineering, vol. 86, no. 1, pp. 9-18. https://doi.org/10.1002/bit.20038

Oxygen gradients in tissue-engineered PEGT/PBT cartilaginous constructs: Measurement and modeling. / Malda, J.; Rouwkema, Jeroen; Martens, D.E.; le Comte, EP; Kooy, F.K.; Tramper, J.; van Blitterswijk, Clemens; Riesle, J.U.

In: Biotechnology and bioengineering, Vol. 86, No. 1, 2004, p. 9-18.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Oxygen gradients in tissue-engineered PEGT/PBT cartilaginous constructs: Measurement and modeling

AU - Malda, J.

AU - Rouwkema, Jeroen

AU - Martens, D.E.

AU - le Comte, EP

AU - Kooy, F.K.

AU - Tramper, J.

AU - van Blitterswijk, Clemens

AU - Riesle, J.U.

PY - 2004

Y1 - 2004

N2 - The supply of oxygen within three-dimensional tissue-engineered (TE) cartilage polymer constructs is mainly by diffusion. Oxygen consumption by cells results in gradients in the oxygen concentration. The aims of this study were, firstly, to identify the gradients within TE cartilage polymer constructs and, secondly, to predict the profiles during in vitro culture. A glass microelectrode system was adapted and used to penetrate cartilage and TE cartilaginous constructs, yielding reproducible measurements with high spatial resolution. Cartilage polymer constructs were cultured for up to 41 days in vitro. Oxygen concentrations, as low as 2-5%, were measured within the center of these constructs. At the beginning of in vitro culture, the oxygen gradients were steeper in TE constructs in comparison to native tissue. Nevertheless, during the course of culture, oxygen concentrations approached the values measured in native tissue. A mathematical model was developed which yields oxygen profiles within cartilage explants and TE constructs. Model input parameters were assessed, including the diffusion coefficient of cartilage (2.2 × 10-9) + (0.4 × 10-9 m2 s-1), 70% of the diffusion coefficient of water and the diffusion coefficient of constructs (3.8 × 10-10 m2 s-1). The model confirmed that chondrocytes in polymer constructs cultured for 27 days have low oxygen requirements (0.8 × 10-19 mol m-3 s-1), even lower than chondrocytes in native cartilage. The ability to measure and predict local oxygen tensions offers new opportunities to obtain more insight in the relation between oxygen tension and chondrogenesis.

AB - The supply of oxygen within three-dimensional tissue-engineered (TE) cartilage polymer constructs is mainly by diffusion. Oxygen consumption by cells results in gradients in the oxygen concentration. The aims of this study were, firstly, to identify the gradients within TE cartilage polymer constructs and, secondly, to predict the profiles during in vitro culture. A glass microelectrode system was adapted and used to penetrate cartilage and TE cartilaginous constructs, yielding reproducible measurements with high spatial resolution. Cartilage polymer constructs were cultured for up to 41 days in vitro. Oxygen concentrations, as low as 2-5%, were measured within the center of these constructs. At the beginning of in vitro culture, the oxygen gradients were steeper in TE constructs in comparison to native tissue. Nevertheless, during the course of culture, oxygen concentrations approached the values measured in native tissue. A mathematical model was developed which yields oxygen profiles within cartilage explants and TE constructs. Model input parameters were assessed, including the diffusion coefficient of cartilage (2.2 × 10-9) + (0.4 × 10-9 m2 s-1), 70% of the diffusion coefficient of water and the diffusion coefficient of constructs (3.8 × 10-10 m2 s-1). The model confirmed that chondrocytes in polymer constructs cultured for 27 days have low oxygen requirements (0.8 × 10-19 mol m-3 s-1), even lower than chondrocytes in native cartilage. The ability to measure and predict local oxygen tensions offers new opportunities to obtain more insight in the relation between oxygen tension and chondrogenesis.

KW - oxygen gradient

KW - Tissue Engineering

KW - METIS-237077

KW - Modeling

KW - Cartilage

KW - Chondrocyte

KW - IR-72009

U2 - 10.1002/bit.20038

DO - 10.1002/bit.20038

M3 - Article

VL - 86

SP - 9

EP - 18

JO - Biotechnology and bioengineering

JF - Biotechnology and bioengineering

SN - 0006-3592

IS - 1

ER -