Oxygen-releasing poly(trimethylene carbonate) microspheres for tissue engineering applications

Hilde Steg; Arina T. Buizer; Willem Woudstra; Albert G. Veldhuizen; Sjoerd K. Bulstra; Dirk W. Grijpma; Roel Kuijer

doi:10.1002/pat.3919

Oxygen-releasing poly(trimethylene carbonate) microspheres for tissue engineering applications

Hilde Steg, Arina T. Buizer, Willem Woudstra, Albert G. Veldhuizen, Sjoerd K. Bulstra, Dirk W. Grijpma, Roel Kuijer^*

^*Corresponding author for this work

Biomaterials Science and Technology

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The introduction of tissue engineering therapies for the repair of bone defects has been limited by poor survival of implanted cells. Because of the absence of a vascular network, the cells in a cell-scaffold construct are not adequately supplied with oxygen and nutrients. Thus far, all but one strategies to solve this problem have failed. Fortunately, oxygen-delivering biomaterials have shown promising results. In this study, composite microspheres comprising a poly(trimethylene carbonate) matrix and calcium peroxide particles (PTMC/CaO₂) were prepared and assessed for their oxygen-delivering capacity and potential cytotoxicity. PTMC/CaO₂ composite microspheres were shown to release oxygen for several weeks. Oxygen release appeared to be dependent on the presence of cholesterol esterase in the medium. The microspheres were not cytotoxic and promoted mesenchymal stromal cell proliferation under hypoxic conditions in vitro.

Original language	English
Pages (from-to)	1252-1257
Number of pages	6
Journal	Polymers for advanced technologies
Volume	28
Issue number	10
DOIs	https://doi.org/10.1002/pat.3919
Publication status	Published - Oct 2017

Keywords

hMSC
microspheres
Oxygen
poly(trimethylene carbonate)
Slow release
2023 OA procedure

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10.1002/pat.3919

ArticleFinal published version, 229 KBLicence: Taverne

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title = "Oxygen-releasing poly(trimethylene carbonate) microspheres for tissue engineering applications",

abstract = "The introduction of tissue engineering therapies for the repair of bone defects has been limited by poor survival of implanted cells. Because of the absence of a vascular network, the cells in a cell-scaffold construct are not adequately supplied with oxygen and nutrients. Thus far, all but one strategies to solve this problem have failed. Fortunately, oxygen-delivering biomaterials have shown promising results. In this study, composite microspheres comprising a poly(trimethylene carbonate) matrix and calcium peroxide particles (PTMC/CaO2) were prepared and assessed for their oxygen-delivering capacity and potential cytotoxicity. PTMC/CaO2 composite microspheres were shown to release oxygen for several weeks. Oxygen release appeared to be dependent on the presence of cholesterol esterase in the medium. The microspheres were not cytotoxic and promoted mesenchymal stromal cell proliferation under hypoxic conditions in vitro.",

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author = "Hilde Steg and Buizer, {Arina T.} and Willem Woudstra and Veldhuizen, {Albert G.} and Bulstra, {Sjoerd K.} and Grijpma, {Dirk W.} and Roel Kuijer",

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AU - Steg, Hilde

AU - Buizer, Arina T.

AU - Woudstra, Willem

AU - Veldhuizen, Albert G.

AU - Bulstra, Sjoerd K.

AU - Grijpma, Dirk W.

AU - Kuijer, Roel

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AB - The introduction of tissue engineering therapies for the repair of bone defects has been limited by poor survival of implanted cells. Because of the absence of a vascular network, the cells in a cell-scaffold construct are not adequately supplied with oxygen and nutrients. Thus far, all but one strategies to solve this problem have failed. Fortunately, oxygen-delivering biomaterials have shown promising results. In this study, composite microspheres comprising a poly(trimethylene carbonate) matrix and calcium peroxide particles (PTMC/CaO2) were prepared and assessed for their oxygen-delivering capacity and potential cytotoxicity. PTMC/CaO2 composite microspheres were shown to release oxygen for several weeks. Oxygen release appeared to be dependent on the presence of cholesterol esterase in the medium. The microspheres were not cytotoxic and promoted mesenchymal stromal cell proliferation under hypoxic conditions in vitro.

KW - hMSC

KW - microspheres

KW - Oxygen

KW - poly(trimethylene carbonate)

KW - Slow release

KW - 2023 OA procedure

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Oxygen-releasing poly(trimethylene carbonate) microspheres for tissue engineering applications

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Keywords

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