Pancreatic islet macroencapsulation using microwell porous membranes

Katarzyna Skrzypek, Milou Groot Nibbelink, Jéré Van Lente, Mijke Buitinga, Marten A. Engelse, Eelco J.P. De Koning, Marcel Karperien, Aart Van Apeldoorn, Dimitrios Stamatialis

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Abstract

Allogeneic islet transplantation into the liver in combination with immune suppressive drug therapy is widely regarded as a potential cure for type 1 diabetes. However, the intrahepatic system is suboptimal as the concentration of drugs and nutrients there is higher compared to pancreas, which negatively affects islet function. Islet encapsulation within semipermeable membranes is a promising strategy that allows for the islet transplantation outside the suboptimal liver portal system and provides environment, where islets can perform their endocrine function. In this study, we develop a macroencapsulation device based on thin microwell membranes. The islets are seeded in separate microwells to avoid aggregation, whereas the membrane porosity is tailored to achieve sufficient transport of nutrients, glucose and insulin. The non-degradable, microwell membranes are composed of poly (ether sulfone)/polyvinylpyrrolidone and manufactured via phase separation micro molding. Our results show that the device prevents aggregation and preserves the islet's native morphology. Moreover, the encapsulated islets maintain their glucose responsiveness and function after 7 days of culture (stimulation index above 2 for high glucose stimulation), demonstrating the potential of this novel device for islet transplantation.

Original languageEnglish
Article number9186
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - 1 Dec 2017

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Islets of Langerhans
Islets of Langerhans Transplantation
Membranes
Glucose
Equipment and Supplies
Portal System
Povidone
Food
Sulfones
Liver
Porosity
Homologous Transplantation
Type 1 Diabetes Mellitus
Ether
Pancreas
Insulin
Drug Therapy
Pharmaceutical Preparations

Cite this

Skrzypek, Katarzyna ; Groot Nibbelink, Milou ; Van Lente, Jéré ; Buitinga, Mijke ; Engelse, Marten A. ; De Koning, Eelco J.P. ; Karperien, Marcel ; Van Apeldoorn, Aart ; Stamatialis, Dimitrios. / Pancreatic islet macroencapsulation using microwell porous membranes. In: Scientific reports. 2017 ; Vol. 7, No. 1.
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Pancreatic islet macroencapsulation using microwell porous membranes. / Skrzypek, Katarzyna; Groot Nibbelink, Milou; Van Lente, Jéré; Buitinga, Mijke; Engelse, Marten A.; De Koning, Eelco J.P.; Karperien, Marcel; Van Apeldoorn, Aart; Stamatialis, Dimitrios.

In: Scientific reports, Vol. 7, No. 1, 9186, 01.12.2017.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Skrzypek, Katarzyna

AU - Groot Nibbelink, Milou

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AU - Engelse, Marten A.

AU - De Koning, Eelco J.P.

AU - Karperien, Marcel

AU - Van Apeldoorn, Aart

AU - Stamatialis, Dimitrios

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