Abstract
We report here a novel and easily scalable microfluidic platform for the parallel
analysis of hundreds of individual cells, with controlled single cell trapping, followed
by their lysis and subsequent retrieval of the cellular content for on-chip analysis.
The device consists of a main channel and an array of shallow side channels connected
to the main channel via trapping structures. Cells are individually captured in
dam structures by application of a negative pressure from an outlet reservoir, lyzed on
site and the cellular content controllably extracted and transported in the individual
side channels for on-chip analysis.
Original language | Undefined |
---|---|
Title of host publication | Micro Total Analysis Systems |
Editors | Tae Song Kim, Yoon-Sik Lee, Taek-Dong Chung, Noo Li Jeon, Sang-Hoon Lee, Kahp-Yang Suh, Jaebum Choo, Yong-Kweon Kim |
Place of Publication | San Diego |
Publisher | Chemical and Biological Society |
Pages | 1808-1810 |
Number of pages | 3 |
ISBN (Print) | 978-0-9798064-2-1 |
Publication status | Published - 1 Nov 2009 |
Event | 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009 - Jeju, Korea, Republic of Duration: 1 Nov 2009 → 5 Nov 2009 Conference number: 13 |
Publication series
Name | microTAS 2009 |
---|---|
Publisher | Chemical and Biological Society |
Volume | 2 |
Conference
Conference | 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009 |
---|---|
Abbreviated title | MicroTAS |
Country/Territory | Korea, Republic of |
City | Jeju |
Period | 1/11/09 → 5/11/09 |
Keywords
- METIS-265248
- IR-69835
- EWI-17032
- platform
- Single cell analysis