Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    1 Citation (Scopus)
    22 Downloads (Pure)

    Abstract

    We report here a novel and easily scalable microfluidic platform for the parallel analysis of hundreds of individual cells, with controlled single cell trapping, followed by their lysis and subsequent retrieval of the cellular content for on-chip analysis. The device consists of a main channel and an array of shallow side channels connected to the main channel via trapping structures. Cells are individually captured in dam structures by application of a negative pressure from an outlet reservoir, lyzed on site and the cellular content controllably extracted and transported in the individual side channels for on-chip analysis.
    Original languageUndefined
    Title of host publicationMicro Total Analysis Systems
    EditorsTae Song Kim, Yoon-Sik Lee, Taek-Dong Chung, Noo Li Jeon, Sang-Hoon Lee, Kahp-Yang Suh, Jaebum Choo, Yong-Kweon Kim
    Place of PublicationSan Diego
    PublisherChemical and Biological Society
    Pages1808-1810
    Number of pages3
    ISBN (Print)978-0-9798064-2-1
    Publication statusPublished - 1 Nov 2009
    Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009 - Jeju, Korea, Republic of
    Duration: 1 Nov 20095 Nov 2009
    Conference number: 13

    Publication series

    NamemicroTAS 2009
    PublisherChemical and Biological Society
    Volume2

    Conference

    Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009
    Abbreviated titleMicroTAS
    CountryKorea, Republic of
    CityJeju
    Period1/11/095/11/09

    Keywords

    • METIS-265248
    • IR-69835
    • EWI-17032
    • platform
    • Single cell analysis

    Cite this

    le Gac, S., de Boer, H. L., Wijnperle, D., Meuleman, W., Carlen, E., & van den Berg, A. (2009). Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis. In T. S. Kim, Y-S. Lee, T-D. Chung, N. L. Jeon, S-H. Lee, K-Y. Suh, J. Choo, ... Y-K. Kim (Eds.), Micro Total Analysis Systems (pp. 1808-1810). (microTAS 2009; Vol. 2). San Diego: Chemical and Biological Society.
    le Gac, Severine ; de Boer, Hans L. ; Wijnperle, Daniël ; Meuleman, W. ; Carlen, Edwin ; van den Berg, Albert. / Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis. Micro Total Analysis Systems. editor / Tae Song Kim ; Yoon-Sik Lee ; Taek-Dong Chung ; Noo Li Jeon ; Sang-Hoon Lee ; Kahp-Yang Suh ; Jaebum Choo ; Yong-Kweon Kim. San Diego : Chemical and Biological Society, 2009. pp. 1808-1810 (microTAS 2009).
    @inproceedings{bd16a6a371a846ee95aa91d6a969b706,
    title = "Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis",
    abstract = "We report here a novel and easily scalable microfluidic platform for the parallel analysis of hundreds of individual cells, with controlled single cell trapping, followed by their lysis and subsequent retrieval of the cellular content for on-chip analysis. The device consists of a main channel and an array of shallow side channels connected to the main channel via trapping structures. Cells are individually captured in dam structures by application of a negative pressure from an outlet reservoir, lyzed on site and the cellular content controllably extracted and transported in the individual side channels for on-chip analysis.",
    keywords = "METIS-265248, IR-69835, EWI-17032, platform, Single cell analysis",
    author = "{le Gac}, Severine and {de Boer}, {Hans L.} and Dani{\"e}l Wijnperle and W. Meuleman and Edwin Carlen and {van den Berg}, Albert",
    year = "2009",
    month = "11",
    day = "1",
    language = "Undefined",
    isbn = "978-0-9798064-2-1",
    series = "microTAS 2009",
    publisher = "Chemical and Biological Society",
    pages = "1808--1810",
    editor = "Kim, {Tae Song} and Yoon-Sik Lee and Taek-Dong Chung and Jeon, {Noo Li} and Sang-Hoon Lee and Kahp-Yang Suh and Jaebum Choo and Yong-Kweon Kim",
    booktitle = "Micro Total Analysis Systems",

    }

    le Gac, S, de Boer, HL, Wijnperle, D, Meuleman, W, Carlen, E & van den Berg, A 2009, Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis. in TS Kim, Y-S Lee, T-D Chung, NL Jeon, S-H Lee, K-Y Suh, J Choo & Y-K Kim (eds), Micro Total Analysis Systems. microTAS 2009, vol. 2, Chemical and Biological Society, San Diego, pp. 1808-1810, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009, Jeju, Korea, Republic of, 1/11/09.

    Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis. / le Gac, Severine; de Boer, Hans L.; Wijnperle, Daniël; Meuleman, W.; Carlen, Edwin; van den Berg, Albert.

    Micro Total Analysis Systems. ed. / Tae Song Kim; Yoon-Sik Lee; Taek-Dong Chung; Noo Li Jeon; Sang-Hoon Lee; Kahp-Yang Suh; Jaebum Choo; Yong-Kweon Kim. San Diego : Chemical and Biological Society, 2009. p. 1808-1810 (microTAS 2009; Vol. 2).

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

    TY - GEN

    T1 - Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis

    AU - le Gac, Severine

    AU - de Boer, Hans L.

    AU - Wijnperle, Daniël

    AU - Meuleman, W.

    AU - Carlen, Edwin

    AU - van den Berg, Albert

    PY - 2009/11/1

    Y1 - 2009/11/1

    N2 - We report here a novel and easily scalable microfluidic platform for the parallel analysis of hundreds of individual cells, with controlled single cell trapping, followed by their lysis and subsequent retrieval of the cellular content for on-chip analysis. The device consists of a main channel and an array of shallow side channels connected to the main channel via trapping structures. Cells are individually captured in dam structures by application of a negative pressure from an outlet reservoir, lyzed on site and the cellular content controllably extracted and transported in the individual side channels for on-chip analysis.

    AB - We report here a novel and easily scalable microfluidic platform for the parallel analysis of hundreds of individual cells, with controlled single cell trapping, followed by their lysis and subsequent retrieval of the cellular content for on-chip analysis. The device consists of a main channel and an array of shallow side channels connected to the main channel via trapping structures. Cells are individually captured in dam structures by application of a negative pressure from an outlet reservoir, lyzed on site and the cellular content controllably extracted and transported in the individual side channels for on-chip analysis.

    KW - METIS-265248

    KW - IR-69835

    KW - EWI-17032

    KW - platform

    KW - Single cell analysis

    M3 - Conference contribution

    SN - 978-0-9798064-2-1

    T3 - microTAS 2009

    SP - 1808

    EP - 1810

    BT - Micro Total Analysis Systems

    A2 - Kim, Tae Song

    A2 - Lee, Yoon-Sik

    A2 - Chung, Taek-Dong

    A2 - Jeon, Noo Li

    A2 - Lee, Sang-Hoon

    A2 - Suh, Kahp-Yang

    A2 - Choo, Jaebum

    A2 - Kim, Yong-Kweon

    PB - Chemical and Biological Society

    CY - San Diego

    ER -

    le Gac S, de Boer HL, Wijnperle D, Meuleman W, Carlen E, van den Berg A. Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis. In Kim TS, Lee Y-S, Chung T-D, Jeon NL, Lee S-H, Suh K-Y, Choo J, Kim Y-K, editors, Micro Total Analysis Systems. San Diego: Chemical and Biological Society. 2009. p. 1808-1810. (microTAS 2009).