Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis

Severine le Gac; Hans L. de Boer; Daniël Wijnperle; W. Meuleman; Edwin Carlen; Albert van den Berg

Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis

Severine le Gac, Hans L. de Boer, Daniël Wijnperle, W. Meuleman, Edwin Carlen, Albert van den Berg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

1 Citation (Scopus)

28 Downloads (Pure)

Abstract

We report here a novel and easily scalable microfluidic platform for the parallel analysis of hundreds of individual cells, with controlled single cell trapping, followed by their lysis and subsequent retrieval of the cellular content for on-chip analysis. The device consists of a main channel and an array of shallow side channels connected to the main channel via trapping structures. Cells are individually captured in dam structures by application of a negative pressure from an outlet reservoir, lyzed on site and the cellular content controllably extracted and transported in the individual side channels for on-chip analysis.

Original language	Undefined
Title of host publication	Micro Total Analysis Systems
Editors	Tae Song Kim, Yoon-Sik Lee, Taek-Dong Chung, Noo Li Jeon, Sang-Hoon Lee, Kahp-Yang Suh, Jaebum Choo, Yong-Kweon Kim
Place of Publication	San Diego
Publisher	Chemical and Biological Society
Pages	1808-1810
Number of pages	3
ISBN (Print)	978-0-9798064-2-1
Publication status	Published - 1 Nov 2009
Event	13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009 - Jeju, Korea, Republic of Duration: 1 Nov 2009 → 5 Nov 2009 Conference number: 13

Publication series

Name	microTAS 2009
Publisher	Chemical and Biological Society
Volume	2

Conference

Conference	13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009
Abbreviated title	MicroTAS
Country	Korea, Republic of
City	Jeju
Period	1/11/09 → 5/11/09

Keywords

METIS-265248
IR-69835
EWI-17032
platform
Single cell analysis

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Cite this

le Gac, S., de Boer, H. L., Wijnperle, D., Meuleman, W., Carlen, E., & van den Berg, A. (2009). Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis. In T. S. Kim, Y-S. Lee, T-D. Chung, N. L. Jeon, S-H. Lee, K-Y. Suh, J. Choo, & Y-K. Kim (Eds.), Micro Total Analysis Systems (pp. 1808-1810). (microTAS 2009; Vol. 2). Chemical and Biological Society.

le Gac, Severine ; de Boer, Hans L. ; Wijnperle, Daniël ; Meuleman, W. ; Carlen, Edwin ; van den Berg, Albert. / Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis. Micro Total Analysis Systems. editor / Tae Song Kim ; Yoon-Sik Lee ; Taek-Dong Chung ; Noo Li Jeon ; Sang-Hoon Lee ; Kahp-Yang Suh ; Jaebum Choo ; Yong-Kweon Kim. San Diego : Chemical and Biological Society, 2009. pp. 1808-1810 (microTAS 2009).

@inproceedings{bd16a6a371a846ee95aa91d6a969b706,

title = "Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis",

abstract = "We report here a novel and easily scalable microfluidic platform for the parallel analysis of hundreds of individual cells, with controlled single cell trapping, followed by their lysis and subsequent retrieval of the cellular content for on-chip analysis. The device consists of a main channel and an array of shallow side channels connected to the main channel via trapping structures. Cells are individually captured in dam structures by application of a negative pressure from an outlet reservoir, lyzed on site and the cellular content controllably extracted and transported in the individual side channels for on-chip analysis.",

keywords = "METIS-265248, IR-69835, EWI-17032, platform, Single cell analysis",

author = "{le Gac}, Severine and {de Boer}, {Hans L.} and Dani{\"e}l Wijnperle and W. Meuleman and Edwin Carlen and {van den Berg}, Albert",

year = "2009",

month = nov,

day = "1",

language = "Undefined",

isbn = "978-0-9798064-2-1",

series = "microTAS 2009",

publisher = "Chemical and Biological Society",

pages = "1808--1810",

editor = "Kim, {Tae Song} and Yoon-Sik Lee and Taek-Dong Chung and Jeon, {Noo Li} and Sang-Hoon Lee and Kahp-Yang Suh and Jaebum Choo and Yong-Kweon Kim",

booktitle = "Micro Total Analysis Systems",

note = "null ; Conference date: 01-11-2009 Through 05-11-2009",

}

le Gac, S, de Boer, HL, Wijnperle, D, Meuleman, W, Carlen, E & van den Berg, A 2009, Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis. in TS Kim, Y-S Lee, T-D Chung, NL Jeon, S-H Lee, K-Y Suh, J Choo & Y-K Kim (eds), Micro Total Analysis Systems. microTAS 2009, vol. 2, Chemical and Biological Society, San Diego, pp. 1808-1810, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009, Jeju, Korea, Republic of, 1/11/09.

Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis. / le Gac, Severine; de Boer, Hans L.; Wijnperle, Daniël; Meuleman, W.; Carlen, Edwin; van den Berg, Albert.

Micro Total Analysis Systems. ed. / Tae Song Kim; Yoon-Sik Lee; Taek-Dong Chung; Noo Li Jeon; Sang-Hoon Lee; Kahp-Yang Suh; Jaebum Choo; Yong-Kweon Kim. San Diego : Chemical and Biological Society, 2009. p. 1808-1810 (microTAS 2009; Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis

AU - le Gac, Severine

AU - de Boer, Hans L.

AU - Wijnperle, Daniël

AU - Meuleman, W.

AU - Carlen, Edwin

AU - van den Berg, Albert

N1 - Conference code: 13

PY - 2009/11/1

Y1 - 2009/11/1

N2 - We report here a novel and easily scalable microfluidic platform for the parallel analysis of hundreds of individual cells, with controlled single cell trapping, followed by their lysis and subsequent retrieval of the cellular content for on-chip analysis. The device consists of a main channel and an array of shallow side channels connected to the main channel via trapping structures. Cells are individually captured in dam structures by application of a negative pressure from an outlet reservoir, lyzed on site and the cellular content controllably extracted and transported in the individual side channels for on-chip analysis.

AB - We report here a novel and easily scalable microfluidic platform for the parallel analysis of hundreds of individual cells, with controlled single cell trapping, followed by their lysis and subsequent retrieval of the cellular content for on-chip analysis. The device consists of a main channel and an array of shallow side channels connected to the main channel via trapping structures. Cells are individually captured in dam structures by application of a negative pressure from an outlet reservoir, lyzed on site and the cellular content controllably extracted and transported in the individual side channels for on-chip analysis.

KW - METIS-265248

KW - IR-69835

KW - EWI-17032

KW - platform

KW - Single cell analysis

M3 - Conference contribution

SN - 978-0-9798064-2-1

T3 - microTAS 2009

SP - 1808

EP - 1810

BT - Micro Total Analysis Systems

A2 - Kim, Tae Song

A2 - Lee, Yoon-Sik

A2 - Chung, Taek-Dong

A2 - Jeon, Noo Li

A2 - Lee, Sang-Hoon

A2 - Suh, Kahp-Yang

A2 - Choo, Jaebum

A2 - Kim, Yong-Kweon

PB - Chemical and Biological Society

CY - San Diego

Y2 - 1 November 2009 through 5 November 2009

ER -

le Gac S, de Boer HL, Wijnperle D, Meuleman W, Carlen E, van den Berg A. Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis. In Kim TS, Lee Y-S, Chung T-D, Jeon NL, Lee S-H, Suh K-Y, Choo J, Kim Y-K, editors, Micro Total Analysis Systems. San Diego: Chemical and Biological Society. 2009. p. 1808-1810. (microTAS 2009).