Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis

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    Abstract

    We report here a novel and easily scalable microfluidic platform for the parallel analysis of hundreds of individual cells, with controlled single cell trapping, followed by their lysis and subsequent retrieval of the cellular content for on-chip analysis. The device consists of a main channel and an array of shallow side channels connected to the main channel via trapping structures. Cells are individually captured in dam structures by application of a negative pressure from an outlet reservoir, lyzed on site and the cellular content controllably extracted and transported in the individual side channels for on-chip analysis.
    Original languageUndefined
    Title of host publicationMicro Total Analysis Systems
    EditorsTae Song Kim, Yoon-Sik Lee, Taek-Dong Chung, Noo Li Jeon, Sang-Hoon Lee, Kahp-Yang Suh, Jaebum Choo, Yong-Kweon Kim
    Place of PublicationSan Diego
    PublisherChemical and Biological Society
    Pages1808-1810
    Number of pages3
    ISBN (Print)978-0-9798064-2-1
    Publication statusPublished - 1 Nov 2009
    Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009 - Jeju, Korea, Republic of
    Duration: 1 Nov 20095 Nov 2009
    Conference number: 13

    Publication series

    NamemicroTAS 2009
    PublisherChemical and Biological Society
    Volume2

    Conference

    Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2009
    Abbreviated titleMicroTAS
    CountryKorea, Republic of
    CityJeju
    Period1/11/095/11/09

    Keywords

    • METIS-265248
    • IR-69835
    • EWI-17032
    • platform
    • Single cell analysis

    Cite this

    le Gac, S., de Boer, H. L., Wijnperle, D., Meuleman, W., Carlen, E., & van den Berg, A. (2009). Parallel single cell analysis on an integrated microfluidic platform for cell trapping, lysis and analysis. In T. S. Kim, Y-S. Lee, T-D. Chung, N. L. Jeon, S-H. Lee, K-Y. Suh, J. Choo, ... Y-K. Kim (Eds.), Micro Total Analysis Systems (pp. 1808-1810). (microTAS 2009; Vol. 2). San Diego: Chemical and Biological Society.